Tags

Type your tag names separated by a space and hit enter

[Influence of electroacupuncture of "Dazhui" (GV 14) "Mingmen" (GV 4) and non-acupoint on the inflammation and immune reactions in adjuvant arthritis rats].
Zhen Ci Yan Jiu 2009; 34(4):225-9ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) on the levels of lymphocyte proliferation rate, spleen and serum interleukin-2 (IL-2), serum tumor necrosis factor-alpha (TNF-alpha), so as to analyze its immuno-regulative function in adjuvant arthritis (AA) rats.

METHODS

A total of 40 Wistar rats were randomized into normal control, model, Dazhui (GV 14), Mingmen (GV 4) and non-acupoint groups. Adjuvant arthritis model was established by subcutaneous injection of Freund's complete adjuvant into the paw. EA was applied to "Dazhui" (GV 14), "Mingmen" (GV 4) and non-acupoint (mid-tail) for 20 min, once every other day, 8 times altogether. Paw volume (swelling degree of the voix pedis) was detected; T and B lymphocyte transformation rates (LTR), and IL-2 activity of the spleen tissue were assayed with MTT chromatometry, and serum IL-2 and TNF-alpha contents were determined by radioimmunoassay.

RESULTS

Compared to normal group, the paw volume and serum TNF-alpha content of model group increased significantly (P < 0:01, P < 0.05), and T- and B-lymphocyte proliferation rates and spleen IL-2 activity in model group decreased considerably (P < 0.01). In comparison with model group, paw volumes of GV 14, GV 4, and non-acupoint groups and serum TNF-alpha content of GV 14 group lowered significantly (P < 0.01, P < 0.05); while T- and B-lymphocyte proliferation rates, spleen IL-2 activity in GV 14, GV 4, and non-acupoint groups, as well as serum IL-2 content in GV 14 and GV 4 groups increased remarkably (P < 0.01). Paw volumes of both GV 14 and GV 4 groups were significantly lower than that of non-acupoint group (P < 0.05); T- and B-lymphocyte proliferation rates and IL-2 activity of GV 4 group were significantly higher than those of non-acupoint group (P < 0.05). No significant differences were found between GV 14 and GV 4 groups in paw volume, T- and B-lymphocyte proliferation rates, spleen IL-2 activity, serum IL-2 and TNF-alpha contents, and between GV 14 and non-acupoint groups in T- and B-lymphocyte proliferation rates, as well as among GV 14, GV 4 and non-acupoint groups in serum IL-2 and TNF-alpha contents (P > 0.05).

CONCLUSION

Electroacupuncture of GV 14 and GV 4 induced immunoregulation on lymphocyte, IL-2 and TNF-alpha may contribute to its anti-inflammation effect in AA rats.

Authors+Show Affiliations

Department of Acu-moxibustion, Sino-Japan Friendship Hospital, Beijing 100029, China. 2008lihui@163.comNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

19916284

Citation

Li, Hui, et al. "[Influence of Electroacupuncture of "Dazhui" (GV 14) "Mingmen" (GV 4) and Non-acupoint On the Inflammation and Immune Reactions in Adjuvant Arthritis Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 34, no. 4, 2009, pp. 225-9.
Li H, Li XH, Zhang LF. [Influence of electroacupuncture of "Dazhui" (GV 14) "Mingmen" (GV 4) and non-acupoint on the inflammation and immune reactions in adjuvant arthritis rats]. Zhen Ci Yan Jiu. 2009;34(4):225-9.
Li, H., Li, X. H., & Zhang, L. F. (2009). [Influence of electroacupuncture of "Dazhui" (GV 14) "Mingmen" (GV 4) and non-acupoint on the inflammation and immune reactions in adjuvant arthritis rats]. Zhen Ci Yan Jiu = Acupuncture Research, 34(4), pp. 225-9.
Li H, Li XH, Zhang LF. [Influence of Electroacupuncture of "Dazhui" (GV 14) "Mingmen" (GV 4) and Non-acupoint On the Inflammation and Immune Reactions in Adjuvant Arthritis Rats]. Zhen Ci Yan Jiu. 2009;34(4):225-9. PubMed PMID: 19916284.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Influence of electroacupuncture of "Dazhui" (GV 14) "Mingmen" (GV 4) and non-acupoint on the inflammation and immune reactions in adjuvant arthritis rats]. AU - Li,Hui, AU - Li,Xiao-Hong, AU - Zhang,Lu-Fen, PY - 2009/11/18/entrez PY - 2009/11/18/pubmed PY - 2010/5/22/medline SP - 225 EP - 9 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 34 IS - 4 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) on the levels of lymphocyte proliferation rate, spleen and serum interleukin-2 (IL-2), serum tumor necrosis factor-alpha (TNF-alpha), so as to analyze its immuno-regulative function in adjuvant arthritis (AA) rats. METHODS: A total of 40 Wistar rats were randomized into normal control, model, Dazhui (GV 14), Mingmen (GV 4) and non-acupoint groups. Adjuvant arthritis model was established by subcutaneous injection of Freund's complete adjuvant into the paw. EA was applied to "Dazhui" (GV 14), "Mingmen" (GV 4) and non-acupoint (mid-tail) for 20 min, once every other day, 8 times altogether. Paw volume (swelling degree of the voix pedis) was detected; T and B lymphocyte transformation rates (LTR), and IL-2 activity of the spleen tissue were assayed with MTT chromatometry, and serum IL-2 and TNF-alpha contents were determined by radioimmunoassay. RESULTS: Compared to normal group, the paw volume and serum TNF-alpha content of model group increased significantly (P < 0:01, P < 0.05), and T- and B-lymphocyte proliferation rates and spleen IL-2 activity in model group decreased considerably (P < 0.01). In comparison with model group, paw volumes of GV 14, GV 4, and non-acupoint groups and serum TNF-alpha content of GV 14 group lowered significantly (P < 0.01, P < 0.05); while T- and B-lymphocyte proliferation rates, spleen IL-2 activity in GV 14, GV 4, and non-acupoint groups, as well as serum IL-2 content in GV 14 and GV 4 groups increased remarkably (P < 0.01). Paw volumes of both GV 14 and GV 4 groups were significantly lower than that of non-acupoint group (P < 0.05); T- and B-lymphocyte proliferation rates and IL-2 activity of GV 4 group were significantly higher than those of non-acupoint group (P < 0.05). No significant differences were found between GV 14 and GV 4 groups in paw volume, T- and B-lymphocyte proliferation rates, spleen IL-2 activity, serum IL-2 and TNF-alpha contents, and between GV 14 and non-acupoint groups in T- and B-lymphocyte proliferation rates, as well as among GV 14, GV 4 and non-acupoint groups in serum IL-2 and TNF-alpha contents (P > 0.05). CONCLUSION: Electroacupuncture of GV 14 and GV 4 induced immunoregulation on lymphocyte, IL-2 and TNF-alpha may contribute to its anti-inflammation effect in AA rats. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/19916284/[Influence_of_electroacupuncture_of_"Dazhui"__GV_14__"Mingmen"__GV_4__and_non_acupoint_on_the_inflammation_and_immune_reactions_in_adjuvant_arthritis_rats]_ DB - PRIME DP - Unbound Medicine ER -