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[Influence of electroacupuncture of Guanyuan (GV 4) and Zusanli (ST 36) on the immune function of T cells in aging rats].
Zhen Ci Yan Jiu. 2009 Aug; 34(4):242-7.ZC

Abstract

OBJECTIVE

To observe the influence of electroacupuncture (EA) on T cell immunologic function in the aged rats so as to reveal its underlying mechanism in anti-aging effect.

METHODS

Fifty SD rats (half male and half female) were randomly divided into normal control (young rats), aging model (model), EA group, immunosuppression (IS), and EA+ IS groups. Aging model (in the late 4 groups) was duplicated by subcutaneous injection of D-galactose for 40 days. Guanyuan (CV 4) and bilateral Zusanli (ST 36) were punctured and stimulated electrically (2 Hz, 1 mA), 15 min every time, six times a week and for four weeks. IS model was induced by i. p. i. of cytoxan (60 mg/kg) after 26 and 27 days' EA treatment. Splenetic T lymphocyte proliferation index was assayed by Methylthiazolyldiphenyl-tetrazolium bromide (MTT) chromatometry. IL-2/IL-2 receptor (R) expression level of T cells was measured by enzyme linked immunosorbent assay (ELISA). CD8+ CD28(+) -T cell subset fluorescence intensity was detected by flow cytometry.

RESULTS

Compared with normal control group, the splenetic T lymphocyte proliferation index, IL-2 and IL-2R expression levels, and CD8+ CD28(+) -T cell density in model and IS groups decreased significantly (P < 0.01). In comparison with model group, the abovementioned indexes in EA and EA + IS groups increased significantly (P < 0.01), and those indexes levels of EA + IS group were also significantly higher than those in IS group (P < 0.05, P < 0.01). Comparison between EA and EA+ IS groups showed that CD8+ CD2+ T cell expression of the later group was significantly lower than that in EA group (P < 0 05). No significant differences were found between EA and EA + IS groups in T cell proliferation rate and IL-2 and IL-2R expression levels (P > 0.05)

CONCLUSION

EA of CV 4 and ST 36 induced upregulation of T cell proliferation and IL-2/IL-2R, and CD8+ CD(+)28 -T cell may contribute to its antiaging and immuno-enhancement effects in the aged rats.

Authors+Show Affiliations

College of Acu-moxibution, Orthopedics and Traumatology, Hubei College of Chinese Medicine, Wuhan 430061, China. liujm_206@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

19916287

Citation

Liu, Jian-Min, et al. "[Influence of Electroacupuncture of Guanyuan (GV 4) and Zusanli (ST 36) On the Immune Function of T Cells in Aging Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 34, no. 4, 2009, pp. 242-7.
Liu JM, Liang FX, Li J, et al. [Influence of electroacupuncture of Guanyuan (GV 4) and Zusanli (ST 36) on the immune function of T cells in aging rats]. Zhen Ci Yan Jiu. 2009;34(4):242-7.
Liu, J. M., Liang, F. X., Li, J., Liu, X. Q., Tang, H. T., Wu, S., Wang, H., & Chen, Z. B. (2009). [Influence of electroacupuncture of Guanyuan (GV 4) and Zusanli (ST 36) on the immune function of T cells in aging rats]. Zhen Ci Yan Jiu = Acupuncture Research, 34(4), 242-7.
Liu JM, et al. [Influence of Electroacupuncture of Guanyuan (GV 4) and Zusanli (ST 36) On the Immune Function of T Cells in Aging Rats]. Zhen Ci Yan Jiu. 2009;34(4):242-7. PubMed PMID: 19916287.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Influence of electroacupuncture of Guanyuan (GV 4) and Zusanli (ST 36) on the immune function of T cells in aging rats]. AU - Liu,Jian-Min, AU - Liang,Feng-Xia, AU - Li,Jia, AU - Liu,Xi-Quan, AU - Tang,Hong-Tu, AU - Wu,Song, AU - Wang,Hua, AU - Chen,Ze-Bin, PY - 2009/11/18/entrez PY - 2009/11/18/pubmed PY - 2010/5/22/medline SP - 242 EP - 7 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 34 IS - 4 N2 - OBJECTIVE: To observe the influence of electroacupuncture (EA) on T cell immunologic function in the aged rats so as to reveal its underlying mechanism in anti-aging effect. METHODS: Fifty SD rats (half male and half female) were randomly divided into normal control (young rats), aging model (model), EA group, immunosuppression (IS), and EA+ IS groups. Aging model (in the late 4 groups) was duplicated by subcutaneous injection of D-galactose for 40 days. Guanyuan (CV 4) and bilateral Zusanli (ST 36) were punctured and stimulated electrically (2 Hz, 1 mA), 15 min every time, six times a week and for four weeks. IS model was induced by i. p. i. of cytoxan (60 mg/kg) after 26 and 27 days' EA treatment. Splenetic T lymphocyte proliferation index was assayed by Methylthiazolyldiphenyl-tetrazolium bromide (MTT) chromatometry. IL-2/IL-2 receptor (R) expression level of T cells was measured by enzyme linked immunosorbent assay (ELISA). CD8+ CD28(+) -T cell subset fluorescence intensity was detected by flow cytometry. RESULTS: Compared with normal control group, the splenetic T lymphocyte proliferation index, IL-2 and IL-2R expression levels, and CD8+ CD28(+) -T cell density in model and IS groups decreased significantly (P < 0.01). In comparison with model group, the abovementioned indexes in EA and EA + IS groups increased significantly (P < 0.01), and those indexes levels of EA + IS group were also significantly higher than those in IS group (P < 0.05, P < 0.01). Comparison between EA and EA+ IS groups showed that CD8+ CD2+ T cell expression of the later group was significantly lower than that in EA group (P < 0 05). No significant differences were found between EA and EA + IS groups in T cell proliferation rate and IL-2 and IL-2R expression levels (P > 0.05) CONCLUSION: EA of CV 4 and ST 36 induced upregulation of T cell proliferation and IL-2/IL-2R, and CD8+ CD(+)28 -T cell may contribute to its antiaging and immuno-enhancement effects in the aged rats. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/19916287/[Influence_of_electroacupuncture_of_Guanyuan__GV_4__and_Zusanli__ST_36__on_the_immune_function_of_T_cells_in_aging_rats]_ L2 - https://medlineplus.gov/olderadulthealth.html DB - PRIME DP - Unbound Medicine ER -