Tags

Type your tag names separated by a space and hit enter

Curcumin nutrition for the prevention of mesenteric ischemia-reperfusion injury: an experimental rodent model.
Transplant Proc. 2009 Nov; 41(9):3611-6.TP

Abstract

BACKGROUND

Curcumin is an anti-oxidant molecule known to be a potent inhibitor of nuclear factor-kappaB (NF-kappaB). It has been shown to attenuate ischemia/reperfusion (I/R) injury in several organ systems. In this study, we sought to investigate the effects of curcumin on the prevention of superior mesenteric artery I/R injury in rats.

METHODS

Wistar albino rats were randomly allocated to 3 groups: group I, sham operated (n = 10); group II, I/R injury only (n = 10); group III, curcumin-treated I/R cohort (n = 10). Group I animals underwent laparotomy without I/R injury. After group II animals underwent laparotomy, 60 minutes of superior mesenteric artery ligation were followed by 3 hours of reperfusion. In the curcumin group, 15 days before I/R, curcumin (40 mg/kg) was administered by gastric gavage. All animals were sacrificed at the end of reperfusion. Intestinal tissue samples were obtained to investigate intestinal mucosal injury; in addition we estimated levels of myeloperoxidase (MPO) activity, malondialdehyde (MDA), nitric oxide (NO), glutathione (GSH), interleukin (IL)-6, and tumor necrosis factor (TNF)-alpha.

RESULTS

There were statistically significant decreases in GSH levels, along with an increase in intestinal mucosal injury scores, MPO activity, MDA levels, NO, IL-6, and TNF-alpha in group I when compared with groups II and III (P = .01). Curcumin treatment in group III produced a significant increase in GSH levels, as well as a decrease in intestinal mucosal injury scores, MPO activity, MDA, and NO levels when compared with group II (P < .05).

CONCLUSION

This study showed that curcumin treatment significantly attenuated reperfusion injury in a superior mesenteric artery I/R model in rats.

Authors+Show Affiliations

Okmeydani Training and Research Hospital, Department of General Surgery, Istanbul, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19917353

Citation

Karatepe, O, et al. "Curcumin Nutrition for the Prevention of Mesenteric Ischemia-reperfusion Injury: an Experimental Rodent Model." Transplantation Proceedings, vol. 41, no. 9, 2009, pp. 3611-6.
Karatepe O, Gulcicek OB, Ugurlucan M, et al. Curcumin nutrition for the prevention of mesenteric ischemia-reperfusion injury: an experimental rodent model. Transplant Proc. 2009;41(9):3611-6.
Karatepe, O., Gulcicek, O. B., Ugurlucan, M., Adas, G., Battal, M., Kemik, A., Kamali, G., Altug, T., & Karahan, S. (2009). Curcumin nutrition for the prevention of mesenteric ischemia-reperfusion injury: an experimental rodent model. Transplantation Proceedings, 41(9), 3611-6. https://doi.org/10.1016/j.transproceed.2009.08.002
Karatepe O, et al. Curcumin Nutrition for the Prevention of Mesenteric Ischemia-reperfusion Injury: an Experimental Rodent Model. Transplant Proc. 2009;41(9):3611-6. PubMed PMID: 19917353.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Curcumin nutrition for the prevention of mesenteric ischemia-reperfusion injury: an experimental rodent model. AU - Karatepe,O, AU - Gulcicek,O B, AU - Ugurlucan,M, AU - Adas,G, AU - Battal,M, AU - Kemik,A, AU - Kamali,G, AU - Altug,T, AU - Karahan,S, PY - 2009/01/13/received PY - 2009/07/17/revised PY - 2009/08/17/accepted PY - 2009/11/18/entrez PY - 2009/11/18/pubmed PY - 2010/2/25/medline SP - 3611 EP - 6 JF - Transplantation proceedings JO - Transplant Proc VL - 41 IS - 9 N2 - BACKGROUND: Curcumin is an anti-oxidant molecule known to be a potent inhibitor of nuclear factor-kappaB (NF-kappaB). It has been shown to attenuate ischemia/reperfusion (I/R) injury in several organ systems. In this study, we sought to investigate the effects of curcumin on the prevention of superior mesenteric artery I/R injury in rats. METHODS: Wistar albino rats were randomly allocated to 3 groups: group I, sham operated (n = 10); group II, I/R injury only (n = 10); group III, curcumin-treated I/R cohort (n = 10). Group I animals underwent laparotomy without I/R injury. After group II animals underwent laparotomy, 60 minutes of superior mesenteric artery ligation were followed by 3 hours of reperfusion. In the curcumin group, 15 days before I/R, curcumin (40 mg/kg) was administered by gastric gavage. All animals were sacrificed at the end of reperfusion. Intestinal tissue samples were obtained to investigate intestinal mucosal injury; in addition we estimated levels of myeloperoxidase (MPO) activity, malondialdehyde (MDA), nitric oxide (NO), glutathione (GSH), interleukin (IL)-6, and tumor necrosis factor (TNF)-alpha. RESULTS: There were statistically significant decreases in GSH levels, along with an increase in intestinal mucosal injury scores, MPO activity, MDA levels, NO, IL-6, and TNF-alpha in group I when compared with groups II and III (P = .01). Curcumin treatment in group III produced a significant increase in GSH levels, as well as a decrease in intestinal mucosal injury scores, MPO activity, MDA, and NO levels when compared with group II (P < .05). CONCLUSION: This study showed that curcumin treatment significantly attenuated reperfusion injury in a superior mesenteric artery I/R model in rats. SN - 1873-2623 UR - https://www.unboundmedicine.com/medline/citation/19917353/Curcumin_nutrition_for_the_prevention_of_mesenteric_ischemia_reperfusion_injury:_an_experimental_rodent_model_ DB - PRIME DP - Unbound Medicine ER -