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Rapid separation and identification of major constituents in Pseudolarix kaempferi by ultra-performance liquid chromatography coupled with electrospray and quadrupole time-of-flight tandem mass spectrometry.
Rapid Commun Mass Spectrom. 2009 Dec; 23(24):3954-62.RC

Abstract

A rapid and reliable method based on ultra-performance liquid chromatography (UPLC) coupled with photodiode-array detection (PDA) and electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF-MS/MS) has been developed for separation and identification of major constituents in extracts of root bark of Pseudolarix kaempferi Gordon (PKG). Identification of the constituents was carried out by interpretation of their retention times, UV absorption spectra, MS and MS/MS spectra, as well as the data provided by authentic standards and literatures. A total of 20 components were separated in only 8.0 min on a small particle size C18 column (1.7 microm). These components included nine diterpene acids, seven glycosides and four triterpenoids, among which pseudolaric acid C-O-beta-D-glucopyranoside and pseudolaric acid C2-O-beta-D-glucopyranoside were separated and identified for the first time in this study. Furthermore, the fragmentation patterns of the three types of compounds were elucidated for the first time. This established UPLC-PDA/Q-TOF-MS/MS method is reliable and effective for the separation and identification of the 20 compounds and will be useful for quality control of the crude materials of Pseudolarix kaempferi Gordon and their related preparations.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Ye X
State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Keyuan Road 4, Gaopeng Street, Chengdu 610041, China.
Tang M
No affiliation info available
Chen L
No affiliation info available
Peng A
No affiliation info available
Ma L
No affiliation info available
Ye H
No affiliation info available

MeSH

Chromatography, High Pressure LiquidDiterpenesGlycosidesPinaceaePlant ExtractsPlant RootsSpectrometry, Mass, Electrospray IonizationTandem Mass Spectrometry

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19918938

Citation

Ye, Xia, et al. "Rapid Separation and Identification of Major Constituents in Pseudolarix Kaempferi By Ultra-performance Liquid Chromatography Coupled With Electrospray and Quadrupole Time-of-flight Tandem Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 23, no. 24, 2009, pp. 3954-62.
Ye X, Tang M, Chen L, et al. Rapid separation and identification of major constituents in Pseudolarix kaempferi by ultra-performance liquid chromatography coupled with electrospray and quadrupole time-of-flight tandem mass spectrometry. Rapid Commun Mass Spectrom. 2009;23(24):3954-62.
Ye, X., Tang, M., Chen, L., Peng, A., Ma, L., & Ye, H. (2009). Rapid separation and identification of major constituents in Pseudolarix kaempferi by ultra-performance liquid chromatography coupled with electrospray and quadrupole time-of-flight tandem mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 23(24), 3954-62. https://doi.org/10.1002/rcm.4336
Ye X, et al. Rapid Separation and Identification of Major Constituents in Pseudolarix Kaempferi By Ultra-performance Liquid Chromatography Coupled With Electrospray and Quadrupole Time-of-flight Tandem Mass Spectrometry. Rapid Commun Mass Spectrom. 2009;23(24):3954-62. PubMed PMID: 19918938.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid separation and identification of major constituents in Pseudolarix kaempferi by ultra-performance liquid chromatography coupled with electrospray and quadrupole time-of-flight tandem mass spectrometry. AU - Ye,Xia, AU - Tang,Minghai, AU - Chen,Lijuan, AU - Peng,Aihua, AU - Ma,Liang, AU - Ye,Haoyu, PY - 2009/11/18/entrez PY - 2009/11/18/pubmed PY - 2010/1/26/medline SP - 3954 EP - 62 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 23 IS - 24 N2 - A rapid and reliable method based on ultra-performance liquid chromatography (UPLC) coupled with photodiode-array detection (PDA) and electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF-MS/MS) has been developed for separation and identification of major constituents in extracts of root bark of Pseudolarix kaempferi Gordon (PKG). Identification of the constituents was carried out by interpretation of their retention times, UV absorption spectra, MS and MS/MS spectra, as well as the data provided by authentic standards and literatures. A total of 20 components were separated in only 8.0 min on a small particle size C18 column (1.7 microm). These components included nine diterpene acids, seven glycosides and four triterpenoids, among which pseudolaric acid C-O-beta-D-glucopyranoside and pseudolaric acid C2-O-beta-D-glucopyranoside were separated and identified for the first time in this study. Furthermore, the fragmentation patterns of the three types of compounds were elucidated for the first time. This established UPLC-PDA/Q-TOF-MS/MS method is reliable and effective for the separation and identification of the 20 compounds and will be useful for quality control of the crude materials of Pseudolarix kaempferi Gordon and their related preparations. SN - 1097-0231 UR - https://www.unboundmedicine.com/medline/citation/19918938/Rapid_separation_and_identification_of_major_constituents_in_Pseudolarix_kaempferi_by_ultra_performance_liquid_chromatography_coupled_with_electrospray_and_quadrupole_time_of_flight_tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -

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