Tags

Type your tag names separated by a space and hit enter

Molecular cloning, mass spectrometric identification, and nutritional evaluation of 10 coixins in adlay (Coix lachryma-jobi L.).
J Agric Food Chem. 2009 Nov 25; 57(22):10916-21.JA

Abstract

Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) is regarded as a nutritive food source as well as herbal medicine. The food nutrition is a consequence of its high protein content and superior amino acid composition. From ca. 200 expressed sequence tag (EST) sequences in maturing adlay grains, clones encoding precursor polypeptides of 10 seed storage proteins in the prolamin family, including 8 alpha-coixin isoforms, 1 delta-coixin, and 1 gamma-coixin, were identified. Full-length cDNA fragments encoding these 10 coixins were obtained by PCR cloning. Mass spectrometric analyses confirmed the presence of these 10 coixins in the extract of adlay grain. Calculated amino acid compositions indicate that all 10 coixins are rich in glutamine (>20% in alpha-coixin isoforms, 13.3% in delta-coixin, and 31.2% in gamma-coixin). The 8 alpha-coixin isoforms are low in methionine, cysteine, and lysine (on average, 0.8, 0.6, and 0.1%, respectively). However, the delta-coixin is a sulfur-rich protein (18.2% methionine and 9.1% cysteine), and the gamma-coixin is a nutritive protein composed of 2.0% methionine, 6.6% cysteine, 2.6% lysine, and 8.9% histidine. The company of delta-coixin and gamma-coixin with alpha-coixin isoforms enhances the nutritional value of alday grain for human consumption.

Authors+Show Affiliations

School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19919123

Citation

Lin, Li-Jen, et al. "Molecular Cloning, Mass Spectrometric Identification, and Nutritional Evaluation of 10 Coixins in Adlay (Coix Lachryma-jobi L.)." Journal of Agricultural and Food Chemistry, vol. 57, no. 22, 2009, pp. 10916-21.
Lin LJ, Hsiao ES, Tseng HS, et al. Molecular cloning, mass spectrometric identification, and nutritional evaluation of 10 coixins in adlay (Coix lachryma-jobi L.). J Agric Food Chem. 2009;57(22):10916-21.
Lin, L. J., Hsiao, E. S., Tseng, H. S., Chung, M. C., Chua, A. C., Kuo, M. E., & Tzen, J. T. (2009). Molecular cloning, mass spectrometric identification, and nutritional evaluation of 10 coixins in adlay (Coix lachryma-jobi L.). Journal of Agricultural and Food Chemistry, 57(22), 10916-21. https://doi.org/10.1021/jf903025n
Lin LJ, et al. Molecular Cloning, Mass Spectrometric Identification, and Nutritional Evaluation of 10 Coixins in Adlay (Coix Lachryma-jobi L.). J Agric Food Chem. 2009 Nov 25;57(22):10916-21. PubMed PMID: 19919123.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cloning, mass spectrometric identification, and nutritional evaluation of 10 coixins in adlay (Coix lachryma-jobi L.). AU - Lin,Li-Jen, AU - Hsiao,Eric S L, AU - Tseng,Hsen-Shong, AU - Chung,Mei-Chu, AU - Chua,Anna C N, AU - Kuo,Ming-En, AU - Tzen,Jason T C, PY - 2009/11/19/entrez PY - 2009/11/19/pubmed PY - 2010/2/19/medline SP - 10916 EP - 21 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 57 IS - 22 N2 - Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) is regarded as a nutritive food source as well as herbal medicine. The food nutrition is a consequence of its high protein content and superior amino acid composition. From ca. 200 expressed sequence tag (EST) sequences in maturing adlay grains, clones encoding precursor polypeptides of 10 seed storage proteins in the prolamin family, including 8 alpha-coixin isoforms, 1 delta-coixin, and 1 gamma-coixin, were identified. Full-length cDNA fragments encoding these 10 coixins were obtained by PCR cloning. Mass spectrometric analyses confirmed the presence of these 10 coixins in the extract of adlay grain. Calculated amino acid compositions indicate that all 10 coixins are rich in glutamine (>20% in alpha-coixin isoforms, 13.3% in delta-coixin, and 31.2% in gamma-coixin). The 8 alpha-coixin isoforms are low in methionine, cysteine, and lysine (on average, 0.8, 0.6, and 0.1%, respectively). However, the delta-coixin is a sulfur-rich protein (18.2% methionine and 9.1% cysteine), and the gamma-coixin is a nutritive protein composed of 2.0% methionine, 6.6% cysteine, 2.6% lysine, and 8.9% histidine. The company of delta-coixin and gamma-coixin with alpha-coixin isoforms enhances the nutritional value of alday grain for human consumption. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/19919123/Molecular_cloning_mass_spectrometric_identification_and_nutritional_evaluation_of_10_coixins_in_adlay__Coix_lachryma_jobi_L___ L2 - https://doi.org/10.1021/jf903025n DB - PRIME DP - Unbound Medicine ER -