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Defensin from disk abalone Haliotis discus discus: molecular cloning, sequence characterization and immune response against bacterial infection.
Fish Shellfish Immunol. 2010 Feb; 28(2):261-6.FS

Abstract

Gene-encoded antimicrobial peptides (AMPs) serve a major role in host defense systems against pathogens. In this study, cDNA of a new mollusk defensin was identified from a normalized cDNA library constructed from whole tissues of disk abalone. Abalone defensin peptide (pro-defensin) has a 198-bp coding sequence comprised of a putative 66 amino acids with a mature defensin consisting of 48 amino acid residues. The presence of an invertebrate defensin family domain, an arrangement of six cysteine residues and their disulfide linkage in C(1)-C(4), C(2)-C(5) and C(3)-C(6) form, an alpha helix in three-dimensional structure and a phylogenetic relationship suggests that abalone defensin could be a new member of the invertebrate defensin family, and related to arthropod defensins. In non-stimulated abalone, defensin transcripts were constitutively expressed in all examined tissues including hemocytes, gills, mantle, muscle, digestive tract and hepatopancreas. It was observed that abalone defensin transcripts were significantly induced in hemocytes, gills and digestive tract at different time intervals after infection by pathogenic bacteria mixture containing Vibrio alginolyticus, Vibrio parahemolyticus and Lysteria monocytogenes. Our overall results suggest that disk abalone defensin could be involved in the immune response reactions as a host defense against pathogenic bacteria.

Authors+Show Affiliations

Department of Marine Life Sciences, College of Ocean Sciences, Jeju National University, Jeju Special Self-Governing Province 690-756, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19922800

Citation

De Zoysa, Mahanama, et al. "Defensin From Disk Abalone Haliotis Discus Discus: Molecular Cloning, Sequence Characterization and Immune Response Against Bacterial Infection." Fish & Shellfish Immunology, vol. 28, no. 2, 2010, pp. 261-6.
De Zoysa M, Whang I, Lee Y, et al. Defensin from disk abalone Haliotis discus discus: molecular cloning, sequence characterization and immune response against bacterial infection. Fish Shellfish Immunol. 2010;28(2):261-6.
De Zoysa, M., Whang, I., Lee, Y., Lee, S., Lee, J. S., & Lee, J. (2010). Defensin from disk abalone Haliotis discus discus: molecular cloning, sequence characterization and immune response against bacterial infection. Fish & Shellfish Immunology, 28(2), 261-6. https://doi.org/10.1016/j.fsi.2009.11.005
De Zoysa M, et al. Defensin From Disk Abalone Haliotis Discus Discus: Molecular Cloning, Sequence Characterization and Immune Response Against Bacterial Infection. Fish Shellfish Immunol. 2010;28(2):261-6. PubMed PMID: 19922800.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Defensin from disk abalone Haliotis discus discus: molecular cloning, sequence characterization and immune response against bacterial infection. AU - De Zoysa,Mahanama, AU - Whang,Ilson, AU - Lee,Youngdeuk, AU - Lee,Sukkyoung, AU - Lee,Jae-Seong, AU - Lee,Jehee, Y1 - 2009/11/14/ PY - 2009/06/29/received PY - 2009/10/25/revised PY - 2009/11/02/accepted PY - 2009/11/20/entrez PY - 2009/11/20/pubmed PY - 2010/3/11/medline SP - 261 EP - 6 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol. VL - 28 IS - 2 N2 - Gene-encoded antimicrobial peptides (AMPs) serve a major role in host defense systems against pathogens. In this study, cDNA of a new mollusk defensin was identified from a normalized cDNA library constructed from whole tissues of disk abalone. Abalone defensin peptide (pro-defensin) has a 198-bp coding sequence comprised of a putative 66 amino acids with a mature defensin consisting of 48 amino acid residues. The presence of an invertebrate defensin family domain, an arrangement of six cysteine residues and their disulfide linkage in C(1)-C(4), C(2)-C(5) and C(3)-C(6) form, an alpha helix in three-dimensional structure and a phylogenetic relationship suggests that abalone defensin could be a new member of the invertebrate defensin family, and related to arthropod defensins. In non-stimulated abalone, defensin transcripts were constitutively expressed in all examined tissues including hemocytes, gills, mantle, muscle, digestive tract and hepatopancreas. It was observed that abalone defensin transcripts were significantly induced in hemocytes, gills and digestive tract at different time intervals after infection by pathogenic bacteria mixture containing Vibrio alginolyticus, Vibrio parahemolyticus and Lysteria monocytogenes. Our overall results suggest that disk abalone defensin could be involved in the immune response reactions as a host defense against pathogenic bacteria. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/19922800/Defensin_from_disk_abalone_Haliotis_discus_discus:_molecular_cloning_sequence_characterization_and_immune_response_against_bacterial_infection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(09)00359-3 DB - PRIME DP - Unbound Medicine ER -