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Identification of polymeric procyanidins from pine bark by mass spectrometry.
Rapid Commun Mass Spectrom. 2009 Dec; 23(24):4013-8.RC

Abstract

Pine bark is an important source of polyphenolic compounds, mainly procyanidins, with reported protective effects against disease. In previous works, barks of two varieties of pine (P. pinaster and P. radiata) were extracted with ethanol, and partially purified to obtain the aqueous fractions (FA), that contained mainly polymeric procyanidins. The mean degree of polymerization was 7.9 for radiata (rFA) and 10.6 for pinaster (pFA). FAs were chromatographed on Sephadex LH-20 by using a gradient of methanol, water and acetone, to render a series of sub-fractions. In this work, the procyanidin compositions of these sub-fractions were analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The mass spectra of sub-fractions from FA of P. pinaster showed signals of procyanidin polymers up to tridecamers, whereas for those from P. radiata the maximum degree of polymerization was 15. For this latter case, the MALDI-TOF mass spectra detected the presence of prodelphinidins in a small amount.

Authors+Show Affiliations

Department of Chemical Engineering, Technical School of Engineering, Avda. Lope Gomez de Marzoa, s/n. 15782 Santiago de Compostela, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19924778

Citation

Jerez, Maria, et al. "Identification of Polymeric Procyanidins From Pine Bark By Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 23, no. 24, 2009, pp. 4013-8.
Jerez M, Sineiro J, Guitián E, et al. Identification of polymeric procyanidins from pine bark by mass spectrometry. Rapid Commun Mass Spectrom. 2009;23(24):4013-8.
Jerez, M., Sineiro, J., Guitián, E., & Núñez, M. J. (2009). Identification of polymeric procyanidins from pine bark by mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 23(24), 4013-8. https://doi.org/10.1002/rcm.4342
Jerez M, et al. Identification of Polymeric Procyanidins From Pine Bark By Mass Spectrometry. Rapid Commun Mass Spectrom. 2009;23(24):4013-8. PubMed PMID: 19924778.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of polymeric procyanidins from pine bark by mass spectrometry. AU - Jerez,Maria, AU - Sineiro,Jorge, AU - Guitián,Esteban, AU - Núñez,Maria J, PY - 2009/11/20/entrez PY - 2009/11/20/pubmed PY - 2010/1/26/medline SP - 4013 EP - 8 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 23 IS - 24 N2 - Pine bark is an important source of polyphenolic compounds, mainly procyanidins, with reported protective effects against disease. In previous works, barks of two varieties of pine (P. pinaster and P. radiata) were extracted with ethanol, and partially purified to obtain the aqueous fractions (FA), that contained mainly polymeric procyanidins. The mean degree of polymerization was 7.9 for radiata (rFA) and 10.6 for pinaster (pFA). FAs were chromatographed on Sephadex LH-20 by using a gradient of methanol, water and acetone, to render a series of sub-fractions. In this work, the procyanidin compositions of these sub-fractions were analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The mass spectra of sub-fractions from FA of P. pinaster showed signals of procyanidin polymers up to tridecamers, whereas for those from P. radiata the maximum degree of polymerization was 15. For this latter case, the MALDI-TOF mass spectra detected the presence of prodelphinidins in a small amount. SN - 1097-0231 UR - https://www.unboundmedicine.com/medline/citation/19924778/Identification_of_polymeric_procyanidins_from_pine_bark_by_mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.4342 DB - PRIME DP - Unbound Medicine ER -