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Determination of amphetamine-type stimulants, ketamine and metabolites in fingernails by gas chromatography-mass spectrometry.
Forensic Sci Int. 2010 Jan 30; 194(1-3):108-14.FS

Abstract

A gas chromatography-mass spectrometry (GC-MS) method was developed and validated for the simultaneous qualification and quantification of methamphetamine (MA), amphetamine (AP), 3,4-methylenedioxy-N-methylamphetamine (MDMA), 3,4-methylenedioxy-N-amphetamine (MDA), ketamine (KET) and norketamine (NKT) in fingernails. Fingernail samples (20mg) were washed with distilled water and methanol, digested with 1.0M sodium hydroxide at 95 degrees C for 30 min, and then extracted with ethyl acetate. Extract solutions were evaporated to dryness, derivatized using heptafluorobutyric anhydride (HFBA) at 60 degrees C for 30 min, and analyzed by GC-MS. The linear ranges were 0.1-20.0 ng/mg for AP, MDMA and NKT, 0.2-20.0 ng/mg for MA and MDA, and 0.4-20.0 ng/mg for KET, with the coefficients of determination (r(2)>or=0.9989). The intra- and inter-day precisions were within 7.1% and 10.6%, respectively. The intra- and inter-day accuracies were -10.9% to 0.8% and -4.3% to 4.5%, respectively. The limits of detections (LODs) and the limits of quantifications (LOQs) for each analyte were lower than 0.094 ng/mg and 0.314 ng/mg, respectively. The recoveries were in the range of 72.3-94.9%. The average fingernail growth rates of two subjects for three years and six subjects for two months were 3.12 mm/month and 3.16 mm/month, respectively. The method proved to be suitable also for the simultaneous detection and quantification of MA, MDMA, KET and their metabolites in fingernails.

Authors+Show Affiliations

Drug Analysis Laboratory, Forensic Science Division, Supreme Prosecutors' Office, Seoul, Republic of Korea. paxus@spo.go.krNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

19926235

Citation

Kim, Jin Young, et al. "Determination of Amphetamine-type Stimulants, Ketamine and Metabolites in Fingernails By Gas Chromatography-mass Spectrometry." Forensic Science International, vol. 194, no. 1-3, 2010, pp. 108-14.
Kim JY, Shin SH, In MK. Determination of amphetamine-type stimulants, ketamine and metabolites in fingernails by gas chromatography-mass spectrometry. Forensic Sci Int. 2010;194(1-3):108-14.
Kim, J. Y., Shin, S. H., & In, M. K. (2010). Determination of amphetamine-type stimulants, ketamine and metabolites in fingernails by gas chromatography-mass spectrometry. Forensic Science International, 194(1-3), 108-14. https://doi.org/10.1016/j.forsciint.2009.10.023
Kim JY, Shin SH, In MK. Determination of Amphetamine-type Stimulants, Ketamine and Metabolites in Fingernails By Gas Chromatography-mass Spectrometry. Forensic Sci Int. 2010 Jan 30;194(1-3):108-14. PubMed PMID: 19926235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of amphetamine-type stimulants, ketamine and metabolites in fingernails by gas chromatography-mass spectrometry. AU - Kim,Jin Young, AU - Shin,Soon Ho, AU - In,Moon Kyo, Y1 - 2009/11/18/ PY - 2009/09/09/received PY - 2009/10/14/revised PY - 2009/10/17/accepted PY - 2009/11/21/entrez PY - 2009/11/21/pubmed PY - 2010/5/6/medline SP - 108 EP - 14 JF - Forensic science international JO - Forensic Sci Int VL - 194 IS - 1-3 N2 - A gas chromatography-mass spectrometry (GC-MS) method was developed and validated for the simultaneous qualification and quantification of methamphetamine (MA), amphetamine (AP), 3,4-methylenedioxy-N-methylamphetamine (MDMA), 3,4-methylenedioxy-N-amphetamine (MDA), ketamine (KET) and norketamine (NKT) in fingernails. Fingernail samples (20mg) were washed with distilled water and methanol, digested with 1.0M sodium hydroxide at 95 degrees C for 30 min, and then extracted with ethyl acetate. Extract solutions were evaporated to dryness, derivatized using heptafluorobutyric anhydride (HFBA) at 60 degrees C for 30 min, and analyzed by GC-MS. The linear ranges were 0.1-20.0 ng/mg for AP, MDMA and NKT, 0.2-20.0 ng/mg for MA and MDA, and 0.4-20.0 ng/mg for KET, with the coefficients of determination (r(2)>or=0.9989). The intra- and inter-day precisions were within 7.1% and 10.6%, respectively. The intra- and inter-day accuracies were -10.9% to 0.8% and -4.3% to 4.5%, respectively. The limits of detections (LODs) and the limits of quantifications (LOQs) for each analyte were lower than 0.094 ng/mg and 0.314 ng/mg, respectively. The recoveries were in the range of 72.3-94.9%. The average fingernail growth rates of two subjects for three years and six subjects for two months were 3.12 mm/month and 3.16 mm/month, respectively. The method proved to be suitable also for the simultaneous detection and quantification of MA, MDMA, KET and their metabolites in fingernails. SN - 1872-6283 UR - https://www.unboundmedicine.com/medline/citation/19926235/Determination_of_amphetamine_type_stimulants_ketamine_and_metabolites_in_fingernails_by_gas_chromatography_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(09)00434-4 DB - PRIME DP - Unbound Medicine ER -