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Association study of cobalamin deficiency in the Chinese Shar Pei.
J Hered. 2010 Mar-Apr; 101(2):211-7.JH

Abstract

Cobalamin deficiency is a common disorder in Chinese Shar Peis (Shar Peis) and is thus suspected to be hereditary. The objective of this study was to identify a genomic region or locus that cosegregates with the phenotype of cobalamin deficiency in Shar Peis. Serum cobalamin concentrations were measured, and blood for genomic DNA extraction was collected from 14 cobalamin-deficient Shar Peis and 28 Shar Peis with a serum cobalamin concentration in the reference range. The 327 microsatellite markers from the canine minimal screening set 2 and 4 additional markers were amplified by polymerase chain reaction and genotyped by automated capillary electrophoresis. Two microsatellite markers, DTR13.6 (P = 1.4 x 10(-6)) and REN13N11 (P = 1.5 x 10(-5)), both on canine chromosome 13, showed evidence of linkage disequilibrium. These findings indicate that the region of chromosome 13 near these markers should be mapped and closely examined for potential mutations associated with this disease in Shar Peis.

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Authors+Show Affiliations

Grützner N
Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843-4474, USA.
Bishop MA
No affiliation info available
Suchodolski JS
No affiliation info available
Steiner JM
No affiliation info available

MeSH

AnimalsCase-Control StudiesChinaChromosomesDog DiseasesDogsFemaleGenetic Association StudiesGenetic LinkageGenetic Predisposition to DiseaseMaleMicrosatellite RepeatsPedigreeSpecies SpecificityVitamin B 12Vitamin B 12 Deficiency

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19926684

Citation

Grützner, Niels, et al. "Association Study of Cobalamin Deficiency in the Chinese Shar Pei." The Journal of Heredity, vol. 101, no. 2, 2010, pp. 211-7.
Grützner N, Bishop MA, Suchodolski JS, et al. Association study of cobalamin deficiency in the Chinese Shar Pei. J Hered. 2010;101(2):211-7.
Grützner, N., Bishop, M. A., Suchodolski, J. S., & Steiner, J. M. (2010). Association study of cobalamin deficiency in the Chinese Shar Pei. The Journal of Heredity, 101(2), 211-7. https://doi.org/10.1093/jhered/esp100
Grützner N, et al. Association Study of Cobalamin Deficiency in the Chinese Shar Pei. J Hered. 2010 Mar-Apr;101(2):211-7. PubMed PMID: 19926684.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association study of cobalamin deficiency in the Chinese Shar Pei. AU - Grützner,Niels, AU - Bishop,Micah A, AU - Suchodolski,Jan S, AU - Steiner,Jörg M, Y1 - 2009/11/19/ PY - 2009/11/21/entrez PY - 2009/11/21/pubmed PY - 2010/5/26/medline SP - 211 EP - 7 JF - The Journal of heredity JO - J Hered VL - 101 IS - 2 N2 - Cobalamin deficiency is a common disorder in Chinese Shar Peis (Shar Peis) and is thus suspected to be hereditary. The objective of this study was to identify a genomic region or locus that cosegregates with the phenotype of cobalamin deficiency in Shar Peis. Serum cobalamin concentrations were measured, and blood for genomic DNA extraction was collected from 14 cobalamin-deficient Shar Peis and 28 Shar Peis with a serum cobalamin concentration in the reference range. The 327 microsatellite markers from the canine minimal screening set 2 and 4 additional markers were amplified by polymerase chain reaction and genotyped by automated capillary electrophoresis. Two microsatellite markers, DTR13.6 (P = 1.4 x 10(-6)) and REN13N11 (P = 1.5 x 10(-5)), both on canine chromosome 13, showed evidence of linkage disequilibrium. These findings indicate that the region of chromosome 13 near these markers should be mapped and closely examined for potential mutations associated with this disease in Shar Peis. SN - 1465-7333 UR - https://www.unboundmedicine.com/medline/citation/19926684/Association_study_of_cobalamin_deficiency_in_the_Chinese_Shar_Pei_ L2 - https://academic.oup.com/jhered/article-lookup/doi/10.1093/jhered/esp100 DB - PRIME DP - Unbound Medicine ER -