Tags

Type your tag names separated by a space and hit enter

Inappropriately high circulating and intrarenal angiotensin II levels during dietary salt loading exacerbate hypertension in Cyp1a1-Ren-2 transgenic rats.
J Hypertens. 2010 Mar; 28(3):495-509.JH

Abstract

OBJECTIVE

We evaluated the effects of salt restriction and of increasing dietary salt loading on blood pressure and the renin-angiotensin system in transgenic rats with inducible hypertension.

METHODS

Hypertension was induced in Cyp1a1-Ren-2 rats through dietary administration of the natural xenobiotic indole-3-carbinol (0.3%), which activates the renin gene. Rats were fed either a normal-salt diet (0.6% NaCl), three different high-salt diets (2, 4 and 8% NaCl) or a low-salt diet (<0.04% NaCl). Blood pressure was monitored by radiotelemetry. Angiotensin II (ANG II) levels were determined by radioimmunoassay.

RESULTS

Induction of the renin gene by administration of indole-3-carbinol resulted in normal-salt diet fed animals in the development of severe hypertension that was accompanied by marked increases in plasma and kidney ANG II levels. Feeding the low-salt diet substantially attenuated the development of hypertension. Treatment with the 2 and 4% high-salt diet did not worsen the course of hypertension and did not alter ANG II levels when compared with rats on the normal salt diet. Feeding the 8% high-salt diet exacerbated the course of hypertension and was associated with further strong increases in plasma and kidney ANG II levels.

CONCLUSION

Our results demonstrate that after induction of the renin gene in Cyp1a1-Ren-2 transgenic rats inappropriate increases in plasma and kidney ANG II levels in response to very high dietary salt intake are responsible for the development of severe hypertension in this model of inducible renin transgenic rats.

Authors+Show Affiliations

Department for Experimental Medicine, Institute for Clinical and Experimental Medicine, 1958/9 Vídenská, Prague, Czech Republic.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19927008

Citation

Husková, Zuzana, et al. "Inappropriately High Circulating and Intrarenal Angiotensin II Levels During Dietary Salt Loading Exacerbate Hypertension in Cyp1a1-Ren-2 Transgenic Rats." Journal of Hypertension, vol. 28, no. 3, 2010, pp. 495-509.
Husková Z, Vanourková Z, Erbanová M, et al. Inappropriately high circulating and intrarenal angiotensin II levels during dietary salt loading exacerbate hypertension in Cyp1a1-Ren-2 transgenic rats. J Hypertens. 2010;28(3):495-509.
Husková, Z., Vanourková, Z., Erbanová, M., Thumová, M., Opocenský, M., Mullins, J. J., Kramer, H. J., Bürgelová, M., & Cervenka, L. (2010). Inappropriately high circulating and intrarenal angiotensin II levels during dietary salt loading exacerbate hypertension in Cyp1a1-Ren-2 transgenic rats. Journal of Hypertension, 28(3), 495-509. https://doi.org/10.1097/HJH.0b013e3283345d69
Husková Z, et al. Inappropriately High Circulating and Intrarenal Angiotensin II Levels During Dietary Salt Loading Exacerbate Hypertension in Cyp1a1-Ren-2 Transgenic Rats. J Hypertens. 2010;28(3):495-509. PubMed PMID: 19927008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inappropriately high circulating and intrarenal angiotensin II levels during dietary salt loading exacerbate hypertension in Cyp1a1-Ren-2 transgenic rats. AU - Husková,Zuzana, AU - Vanourková,Zdenka, AU - Erbanová,Michaela, AU - Thumová,Monika, AU - Opocenský,Martin, AU - Mullins,John J, AU - Kramer,Herbert J, AU - Bürgelová,Marcela, AU - Cervenka,Ludek, PY - 2009/11/21/entrez PY - 2009/11/21/pubmed PY - 2010/6/2/medline SP - 495 EP - 509 JF - Journal of hypertension JO - J. Hypertens. VL - 28 IS - 3 N2 - OBJECTIVE: We evaluated the effects of salt restriction and of increasing dietary salt loading on blood pressure and the renin-angiotensin system in transgenic rats with inducible hypertension. METHODS: Hypertension was induced in Cyp1a1-Ren-2 rats through dietary administration of the natural xenobiotic indole-3-carbinol (0.3%), which activates the renin gene. Rats were fed either a normal-salt diet (0.6% NaCl), three different high-salt diets (2, 4 and 8% NaCl) or a low-salt diet (<0.04% NaCl). Blood pressure was monitored by radiotelemetry. Angiotensin II (ANG II) levels were determined by radioimmunoassay. RESULTS: Induction of the renin gene by administration of indole-3-carbinol resulted in normal-salt diet fed animals in the development of severe hypertension that was accompanied by marked increases in plasma and kidney ANG II levels. Feeding the low-salt diet substantially attenuated the development of hypertension. Treatment with the 2 and 4% high-salt diet did not worsen the course of hypertension and did not alter ANG II levels when compared with rats on the normal salt diet. Feeding the 8% high-salt diet exacerbated the course of hypertension and was associated with further strong increases in plasma and kidney ANG II levels. CONCLUSION: Our results demonstrate that after induction of the renin gene in Cyp1a1-Ren-2 transgenic rats inappropriate increases in plasma and kidney ANG II levels in response to very high dietary salt intake are responsible for the development of severe hypertension in this model of inducible renin transgenic rats. SN - 1473-5598 UR - https://www.unboundmedicine.com/medline/citation/19927008/Inappropriately_high_circulating_and_intrarenal_angiotensin_II_levels_during_dietary_salt_loading_exacerbate_hypertension_in_Cyp1a1_Ren_2_transgenic_rats_ L2 - http://dx.doi.org/10.1097/HJH.0b013e3283345d69 DB - PRIME DP - Unbound Medicine ER -