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In vivo effects of meloxicam on inflammatory mediators, MMP activity and cartilage biomarkers in equine joints with acute synovitis.
Equine Vet J. 2009 Sep; 41(7):693-9.EV

Abstract

REASONS FOR PERFORMING STUDY

Meloxicam is a commonly used nonsteroidal anti-inflammatory drug in equine practice, but little is known about its in vivo effects on joint inflammation and cartilage turnover.

OBJECTIVES

To study the effects of meloxicam on biomarkers of inflammation, matrix metalloproteinase (MMP) activity, and cartilage biomarkers in joints with experimental synovitis.

METHODS

In a 2-period cross-over study, synovitis was induced at T = 0 h in the L or R intercarpal joint of 6 horses by intraarticular injection of 0.5 ng lipopolysaccharide (LPS). Horses received once daily meloxicam (0.6 mg/kg bwt per os) or placebo starting at post injection hour (PIH) 2, and clinical evaluations as well as blood and synovial fluid (SF) sampling were performed at PIH 0, 8, 24 and 168. Synovial fluid was analysed for prostaglandin E2, bradykinin, substance P, general MMP activity, glycosaminoglycans (GAG), CS846 epitope, type II collagen cleavage fragments (C2C) and type II collagen carboxypropeptide (CPII). Concentrations in meloxicam- vs. placebo-treated joints over time were compared using a linear mixed model.

RESULTS

Lipopolysaccharide injection caused marked transient synovitis without systemic effects. Meloxicam caused a significant reduction in lameness at PIH 8 and 24 and tended to reduce effusion. In addition, meloxicam significantly suppressed SF prostaglandin E2 and substance P release at PIH 8 and bradykinin at PIH 24 compared to placebo treatment. General MMP activity at PIH 8 and 24 was significantly lower in meloxicam- vs. placebo-treated joints, as were GAG, C2C and CPII concentrations at PIH 24.

CONCLUSIONS

Acute transient synovitis leads to substantial increases in SF biomarkers of inflammation, MMP activity and cartilage turnover, which can be significantly suppressed by meloxicam.

POTENTIAL RELEVANCE

Early oral treatment with meloxicam ameliorates not only clinical signs and joint inflammation in acute synovitis, but may also limit inflammation-induced cartilage catabolism.

Authors+Show Affiliations

Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19927589

Citation

de Grauw, J C., et al. "In Vivo Effects of Meloxicam On Inflammatory Mediators, MMP Activity and Cartilage Biomarkers in Equine Joints With Acute Synovitis." Equine Veterinary Journal, vol. 41, no. 7, 2009, pp. 693-9.
de Grauw JC, van de Lest CH, Brama PA, et al. In vivo effects of meloxicam on inflammatory mediators, MMP activity and cartilage biomarkers in equine joints with acute synovitis. Equine Vet J. 2009;41(7):693-9.
de Grauw, J. C., van de Lest, C. H., Brama, P. A., Rambags, B. P., & van Weeren, P. R. (2009). In vivo effects of meloxicam on inflammatory mediators, MMP activity and cartilage biomarkers in equine joints with acute synovitis. Equine Veterinary Journal, 41(7), 693-9.
de Grauw JC, et al. In Vivo Effects of Meloxicam On Inflammatory Mediators, MMP Activity and Cartilage Biomarkers in Equine Joints With Acute Synovitis. Equine Vet J. 2009;41(7):693-9. PubMed PMID: 19927589.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo effects of meloxicam on inflammatory mediators, MMP activity and cartilage biomarkers in equine joints with acute synovitis. AU - de Grauw,J C, AU - van de Lest,C H A, AU - Brama,P A J, AU - Rambags,B P B, AU - van Weeren,P R, PY - 2009/11/26/entrez PY - 2009/11/26/pubmed PY - 2010/1/6/medline SP - 693 EP - 9 JF - Equine veterinary journal JO - Equine Vet. J. VL - 41 IS - 7 N2 - REASONS FOR PERFORMING STUDY: Meloxicam is a commonly used nonsteroidal anti-inflammatory drug in equine practice, but little is known about its in vivo effects on joint inflammation and cartilage turnover. OBJECTIVES: To study the effects of meloxicam on biomarkers of inflammation, matrix metalloproteinase (MMP) activity, and cartilage biomarkers in joints with experimental synovitis. METHODS: In a 2-period cross-over study, synovitis was induced at T = 0 h in the L or R intercarpal joint of 6 horses by intraarticular injection of 0.5 ng lipopolysaccharide (LPS). Horses received once daily meloxicam (0.6 mg/kg bwt per os) or placebo starting at post injection hour (PIH) 2, and clinical evaluations as well as blood and synovial fluid (SF) sampling were performed at PIH 0, 8, 24 and 168. Synovial fluid was analysed for prostaglandin E2, bradykinin, substance P, general MMP activity, glycosaminoglycans (GAG), CS846 epitope, type II collagen cleavage fragments (C2C) and type II collagen carboxypropeptide (CPII). Concentrations in meloxicam- vs. placebo-treated joints over time were compared using a linear mixed model. RESULTS: Lipopolysaccharide injection caused marked transient synovitis without systemic effects. Meloxicam caused a significant reduction in lameness at PIH 8 and 24 and tended to reduce effusion. In addition, meloxicam significantly suppressed SF prostaglandin E2 and substance P release at PIH 8 and bradykinin at PIH 24 compared to placebo treatment. General MMP activity at PIH 8 and 24 was significantly lower in meloxicam- vs. placebo-treated joints, as were GAG, C2C and CPII concentrations at PIH 24. CONCLUSIONS: Acute transient synovitis leads to substantial increases in SF biomarkers of inflammation, MMP activity and cartilage turnover, which can be significantly suppressed by meloxicam. POTENTIAL RELEVANCE: Early oral treatment with meloxicam ameliorates not only clinical signs and joint inflammation in acute synovitis, but may also limit inflammation-induced cartilage catabolism. SN - 0425-1644 UR - https://www.unboundmedicine.com/medline/citation/19927589/In_vivo_effects_of_meloxicam_on_inflammatory_mediators_MMP_activity_and_cartilage_biomarkers_in_equine_joints_with_acute_synovitis_ L2 - https://doi.org/10.2746/042516409x436286 DB - PRIME DP - Unbound Medicine ER -