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High-performance liquid chromatography-ultraviolet detection method for the simultaneous determination of typical biogenic amines and precursor amino acids. Applications in food chemistry.
J Agric Food Chem. 2010 Jan 13; 58(1):127-34.JA

Abstract

A reversed-phase high-performance liquid chromatography (HPLC) method was developed for the simultaneous determination in food of biogenic amines and their precursor amino acids after a precolumn derivatization with dansyl chloride. The chromatographic conditions, selected to be suitable for mass spectrometry detection, were optimized through experimental design and artificial neural networks. The HPLC-UV method was validated by comparing the separation results with those obtained through a HPLC method, working under the same chromatographic conditions but employing mass spectrometry detection. The HPLC-UV method was then applied to the analysis of different food samples, namely, cheese, clams, salami, and beer. For all of the matrices, recoveries (relative standard deviation always <5%) always >92% were obtained. The results are discussed as a function of the total biogenic amine content and of the concentration ratio between amines and precursor amino acids.

Authors+Show Affiliations

DISAV Dipartimento di Scienze dell'Ambiente e della Vita, University of Piemonte Orientale Amedeo Avogadro, Viale Teresa Michel 11, 15121 Alessandria, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19928988

Citation

Mazzucco, Eleonora, et al. "High-performance Liquid Chromatography-ultraviolet Detection Method for the Simultaneous Determination of Typical Biogenic Amines and Precursor Amino Acids. Applications in Food Chemistry." Journal of Agricultural and Food Chemistry, vol. 58, no. 1, 2010, pp. 127-34.
Mazzucco E, Gosetti F, Bobba M, et al. High-performance liquid chromatography-ultraviolet detection method for the simultaneous determination of typical biogenic amines and precursor amino acids. Applications in food chemistry. J Agric Food Chem. 2010;58(1):127-34.
Mazzucco, E., Gosetti, F., Bobba, M., Marengo, E., Robotti, E., & Gennaro, M. C. (2010). High-performance liquid chromatography-ultraviolet detection method for the simultaneous determination of typical biogenic amines and precursor amino acids. Applications in food chemistry. Journal of Agricultural and Food Chemistry, 58(1), 127-34. https://doi.org/10.1021/jf9030053
Mazzucco E, et al. High-performance Liquid Chromatography-ultraviolet Detection Method for the Simultaneous Determination of Typical Biogenic Amines and Precursor Amino Acids. Applications in Food Chemistry. J Agric Food Chem. 2010 Jan 13;58(1):127-34. PubMed PMID: 19928988.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-performance liquid chromatography-ultraviolet detection method for the simultaneous determination of typical biogenic amines and precursor amino acids. Applications in food chemistry. AU - Mazzucco,Eleonora, AU - Gosetti,Fabio, AU - Bobba,Marco, AU - Marengo,Emilio, AU - Robotti,Elisa, AU - Gennaro,Maria Carla, PY - 2009/11/26/entrez PY - 2009/11/26/pubmed PY - 2010/3/23/medline SP - 127 EP - 34 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 58 IS - 1 N2 - A reversed-phase high-performance liquid chromatography (HPLC) method was developed for the simultaneous determination in food of biogenic amines and their precursor amino acids after a precolumn derivatization with dansyl chloride. The chromatographic conditions, selected to be suitable for mass spectrometry detection, were optimized through experimental design and artificial neural networks. The HPLC-UV method was validated by comparing the separation results with those obtained through a HPLC method, working under the same chromatographic conditions but employing mass spectrometry detection. The HPLC-UV method was then applied to the analysis of different food samples, namely, cheese, clams, salami, and beer. For all of the matrices, recoveries (relative standard deviation always <5%) always >92% were obtained. The results are discussed as a function of the total biogenic amine content and of the concentration ratio between amines and precursor amino acids. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/19928988/High_performance_liquid_chromatography_ultraviolet_detection_method_for_the_simultaneous_determination_of_typical_biogenic_amines_and_precursor_amino_acids__Applications_in_food_chemistry_ L2 - https://dx.doi.org/10.1021/jf9030053 DB - PRIME DP - Unbound Medicine ER -