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Heterologous expression of Arabidopsis H+-pyrophosphatase enhances salt tolerance in transgenic creeping bentgrass (Agrostis stolonifera L.).
Plant Cell Environ. 2010 Feb; 33(2):272-89.PC

Abstract

The Arabidopsis vacuolar H(+)-pyrophosphatase (AVP1), when over-expressed in transgenic (TG) plants, regulates root and shoot development via facilitation of auxin flux, and enhances plant resistance to salt and drought stresses. Here, we report that TG perennial creeping bentgrass plants over-expressing AVP1 exhibited improved resistance to salinity than wild-type (WT) controls. Compared to WT plants, TGs grew well in the presence of 100 mm NaCl, and exhibited higher tolerance and faster recovery from damages from exposure to 200 and 300 mm NaCl. The improved performance of the TG plants was associated with higher relative water content (RWC), higher Na(+) uptake and lower solute leakage in leaf tissues, and with higher concentrations of Na(+), K(+), Cl(-) and total phosphorus in root tissues. Under salt stress, proline content was increased in both WT and TG plants, but more significantly in TGs. Moreover, TG plants exhibited greater biomass production than WT controls under both normal and elevated salinity conditions. When subjected to salt stress, fresh (FW) and dry weights (DW) of both leaves and roots decreased more significantly in WT than in TG plants. Our results demonstrated the great potential of genetic manipulation of vacuolar H(+)-pyrophosphatase expression in TG perennial species for improvement of plant abiotic stress resistance.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Li Z
Department of Genetics and Biochemistry, 100 Jordan Hall, Clemson University, Clemson, SC 29634, USA.
Baldwin CM
No affiliation info available
Hu Q
No affiliation info available
Liu H
No affiliation info available
Luo H
No affiliation info available

MeSH

AgrostisArabidopsisArabidopsis ProteinsChloridesChlorophyllGene Expression Regulation, PlantIndoleacetic AcidsInorganic PyrophosphatasePhosphorusPlant LeavesPlant RootsPlants, Genetically ModifiedPotassiumSalt-Tolerant PlantsSodiumStress, PhysiologicalTransformation, GeneticWater

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19930128

Citation

Li, Zhigang, et al. "Heterologous Expression of Arabidopsis H+-pyrophosphatase Enhances Salt Tolerance in Transgenic Creeping Bentgrass (Agrostis Stolonifera L.)." Plant, Cell & Environment, vol. 33, no. 2, 2010, pp. 272-89.
Li Z, Baldwin CM, Hu Q, et al. Heterologous expression of Arabidopsis H+-pyrophosphatase enhances salt tolerance in transgenic creeping bentgrass (Agrostis stolonifera L.). Plant Cell Environ. 2010;33(2):272-89.
Li, Z., Baldwin, C. M., Hu, Q., Liu, H., & Luo, H. (2010). Heterologous expression of Arabidopsis H+-pyrophosphatase enhances salt tolerance in transgenic creeping bentgrass (Agrostis stolonifera L.). Plant, Cell & Environment, 33(2), 272-89. https://doi.org/10.1111/j.1365-3040.2009.02080.x
Li Z, et al. Heterologous Expression of Arabidopsis H+-pyrophosphatase Enhances Salt Tolerance in Transgenic Creeping Bentgrass (Agrostis Stolonifera L.). Plant Cell Environ. 2010;33(2):272-89. PubMed PMID: 19930128.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Heterologous expression of Arabidopsis H+-pyrophosphatase enhances salt tolerance in transgenic creeping bentgrass (Agrostis stolonifera L.). AU - Li,Zhigang, AU - Baldwin,Christian M, AU - Hu,Qian, AU - Liu,Haibo, AU - Luo,Hong, Y1 - 2009/11/17/ PY - 2009/11/26/entrez PY - 2009/11/26/pubmed PY - 2010/7/10/medline SP - 272 EP - 89 JF - Plant, cell & environment JO - Plant Cell Environ VL - 33 IS - 2 N2 - The Arabidopsis vacuolar H(+)-pyrophosphatase (AVP1), when over-expressed in transgenic (TG) plants, regulates root and shoot development via facilitation of auxin flux, and enhances plant resistance to salt and drought stresses. Here, we report that TG perennial creeping bentgrass plants over-expressing AVP1 exhibited improved resistance to salinity than wild-type (WT) controls. Compared to WT plants, TGs grew well in the presence of 100 mm NaCl, and exhibited higher tolerance and faster recovery from damages from exposure to 200 and 300 mm NaCl. The improved performance of the TG plants was associated with higher relative water content (RWC), higher Na(+) uptake and lower solute leakage in leaf tissues, and with higher concentrations of Na(+), K(+), Cl(-) and total phosphorus in root tissues. Under salt stress, proline content was increased in both WT and TG plants, but more significantly in TGs. Moreover, TG plants exhibited greater biomass production than WT controls under both normal and elevated salinity conditions. When subjected to salt stress, fresh (FW) and dry weights (DW) of both leaves and roots decreased more significantly in WT than in TG plants. Our results demonstrated the great potential of genetic manipulation of vacuolar H(+)-pyrophosphatase expression in TG perennial species for improvement of plant abiotic stress resistance. SN - 1365-3040 UR - https://www.unboundmedicine.com/medline/citation/19930128/Heterologous_expression_of_Arabidopsis_H+_pyrophosphatase_enhances_salt_tolerance_in_transgenic_creeping_bentgrass__Agrostis_stolonifera_L___ DB - PRIME DP - Unbound Medicine ER -