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[Mechanism of inhibition of apoptosis of human umbilical vein endothelial cells induced by oxidized-low density lipoprotein by erythropoietin].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2009 Nov; 21(11):656-9.ZW

Abstract

OBJECTIVE

To explore the protective effect of erythropoietin (EPO) against oxidized-low density lipoprotein (ox-LDL)-induced apoptosis in human umbilical vein endothelial cells (HUVECs) in an ox-LDL induced apoptosis model.

METHODS

Third-sixth passage of cultured HUVECs were used, and they were divided into two groups. The cells were pretreated with different concentrations (6.25, 50, 100 kU/L) of recombinant human erythropoietin (rhEPO) for 24 hours, then they were exposed to ox-LDL (100 mg/L) for 48 hours; another group of cells were pretreated with antisense to 0.5 micromol/L LOX-1 mRNA or 0.5 micromol/L sense for 24 hours, and then HUVECs were exposed to ox-LDL (100 mg/L) for 12 hours. Apoptosis was assessed by the apoptosis ratio, cell viability, and Bcl-2/Bax ratio.

RESULTS

As compared to untreated controls, pretreatment with rhEPO led to increased cell survival of HUVECs and decreased cell apoptosis in a dose-dependent manner (all P<0.05). Consistently, the Bcl-2/Bax ratios were also increased in a similar fashion. The ratio of apoptosis protein Bcl-2/Bax was increased in the antisense LOX-1 mRNA group than that of ox-LDL group (P<0.05), but the one in the sense LOX-1 mRNA group was not significantly different from that of ox-LDL group.

CONCLUSION

The ox-LDL can induce apoptosis in HUVECs by regulating LOX-1 mRNA, and rhEPO can increase Bcl-2/Bax ratio and inhibit ox-LDL-induced apoptosis of HUVECs.

Authors+Show Affiliations

Department of Cardiology, First Hospital of Lanzhou University, Key Lab of Preclinical Study for New Drugs of Gansu Province, Lanzhou 730000, Gansu, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

19930880

Citation

Yang, Xiao-fang, et al. "[Mechanism of Inhibition of Apoptosis of Human Umbilical Vein Endothelial Cells Induced By Oxidized-low Density Lipoprotein By Erythropoietin]." Zhongguo Wei Zhong Bing Ji Jiu Yi Xue = Chinese Critical Care Medicine = Zhongguo Weizhongbing Jijiuyixue, vol. 21, no. 11, 2009, pp. 656-9.
Yang XF, Xiong JW, Wang ZL, et al. [Mechanism of inhibition of apoptosis of human umbilical vein endothelial cells induced by oxidized-low density lipoprotein by erythropoietin]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2009;21(11):656-9.
Yang, X. F., Xiong, J. W., Wang, Z. L., Li, Y., Xu, Z. T., Cui, L. J., Wang, F., Tan, L. L., & Zhang, L. (2009). [Mechanism of inhibition of apoptosis of human umbilical vein endothelial cells induced by oxidized-low density lipoprotein by erythropoietin]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue = Chinese Critical Care Medicine = Zhongguo Weizhongbing Jijiuyixue, 21(11), 656-9.
Yang XF, et al. [Mechanism of Inhibition of Apoptosis of Human Umbilical Vein Endothelial Cells Induced By Oxidized-low Density Lipoprotein By Erythropoietin]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2009;21(11):656-9. PubMed PMID: 19930880.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Mechanism of inhibition of apoptosis of human umbilical vein endothelial cells induced by oxidized-low density lipoprotein by erythropoietin]. AU - Yang,Xiao-fang, AU - Xiong,Jian-wen, AU - Wang,Zhi-lu, AU - Li,Yan, AU - Xu,Zhe-tong, AU - Cui,Li-jun, AU - Wang,Feng, AU - Tan,Li-li, AU - Zhang,Li, PY - 2009/11/26/entrez PY - 2009/11/26/pubmed PY - 2010/11/3/medline SP - 656 EP - 9 JF - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue JO - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue VL - 21 IS - 11 N2 - OBJECTIVE: To explore the protective effect of erythropoietin (EPO) against oxidized-low density lipoprotein (ox-LDL)-induced apoptosis in human umbilical vein endothelial cells (HUVECs) in an ox-LDL induced apoptosis model. METHODS: Third-sixth passage of cultured HUVECs were used, and they were divided into two groups. The cells were pretreated with different concentrations (6.25, 50, 100 kU/L) of recombinant human erythropoietin (rhEPO) for 24 hours, then they were exposed to ox-LDL (100 mg/L) for 48 hours; another group of cells were pretreated with antisense to 0.5 micromol/L LOX-1 mRNA or 0.5 micromol/L sense for 24 hours, and then HUVECs were exposed to ox-LDL (100 mg/L) for 12 hours. Apoptosis was assessed by the apoptosis ratio, cell viability, and Bcl-2/Bax ratio. RESULTS: As compared to untreated controls, pretreatment with rhEPO led to increased cell survival of HUVECs and decreased cell apoptosis in a dose-dependent manner (all P<0.05). Consistently, the Bcl-2/Bax ratios were also increased in a similar fashion. The ratio of apoptosis protein Bcl-2/Bax was increased in the antisense LOX-1 mRNA group than that of ox-LDL group (P<0.05), but the one in the sense LOX-1 mRNA group was not significantly different from that of ox-LDL group. CONCLUSION: The ox-LDL can induce apoptosis in HUVECs by regulating LOX-1 mRNA, and rhEPO can increase Bcl-2/Bax ratio and inhibit ox-LDL-induced apoptosis of HUVECs. SN - 1003-0603 UR - https://www.unboundmedicine.com/medline/citation/19930880/[Mechanism_of_inhibition_of_apoptosis_of_human_umbilical_vein_endothelial_cells_induced_by_oxidized_low_density_lipoprotein_by_erythropoietin]_ L2 - https://antibodies.cancer.gov/detail/CPTC-TIMP1-2 DB - PRIME DP - Unbound Medicine ER -