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Polysaccharides from Sargassum tenerrimum: structural features, chemical modification and anti-viral activity.
Phytochemistry. 2010 Feb; 71(2-3):235-42.P

Abstract

Herpes simplex viruses (HSVs) display affinity for cell-surface heparan sulfate proteoglycans with biological relevance in virus entry. Here, we exploit an approach to inhibiting HSV infection by using a sulfated fucoidan, and a guluronic acid-rich alginate derived from Sargassum tenerrimum, mimicking the active domain of the entry receptor. These macromolecules have apparent molecular masses of 30+/-5 and 26+/-5 kDa, respectively. They and their chemically sulfated derivatives showed activity against herpes simplex virus type 1 (HSV-1). Their inhibitory concentration 50% (IC(50)) values were in the range 0.5-15 microg/ml and they lacked cytotoxicity at concentrations up to 1000 microg/ml. The anti-HSV activity increased with increasing sulfate ester content. Our results suggest the feasibility of inhibiting HSV infection by blocking viral entry with polysaccharide having specific structure.

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Authors+Show Affiliations

Sinha S
Natural Products Laboratory, Department of Chemistry, The University of Burdwan, West Bengal 713104, India.
Astani A
No affiliation info available
Ghosh T
No affiliation info available
Schnitzler P
No affiliation info available
Ray B
No affiliation info available

MeSH

AlginatesAnimalsAntiviral AgentsCells, CulturedGlucuronic AcidHaplorhiniHerpes SimplexHerpesvirus 1, HumanHexuronic AcidsInhibitory Concentration 50PhytotherapyPlant ExtractsPolysaccharidesSargassumStructure-Activity RelationshipVirus Integration

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19931103

Citation

Sinha, Sharmistha, et al. "Polysaccharides From Sargassum Tenerrimum: Structural Features, Chemical Modification and Anti-viral Activity." Phytochemistry, vol. 71, no. 2-3, 2010, pp. 235-42.
Sinha S, Astani A, Ghosh T, et al. Polysaccharides from Sargassum tenerrimum: structural features, chemical modification and anti-viral activity. Phytochemistry. 2010;71(2-3):235-42.
Sinha, S., Astani, A., Ghosh, T., Schnitzler, P., & Ray, B. (2010). Polysaccharides from Sargassum tenerrimum: structural features, chemical modification and anti-viral activity. Phytochemistry, 71(2-3), 235-42. https://doi.org/10.1016/j.phytochem.2009.10.014
Sinha S, et al. Polysaccharides From Sargassum Tenerrimum: Structural Features, Chemical Modification and Anti-viral Activity. Phytochemistry. 2010;71(2-3):235-42. PubMed PMID: 19931103.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polysaccharides from Sargassum tenerrimum: structural features, chemical modification and anti-viral activity. AU - Sinha,Sharmistha, AU - Astani,Akram, AU - Ghosh,Tuhin, AU - Schnitzler,Paul, AU - Ray,Bimalendu, PY - 2009/08/26/received PY - 2009/10/16/revised PY - 2009/10/16/accepted PY - 2009/11/26/entrez PY - 2009/11/26/pubmed PY - 2010/8/11/medline SP - 235 EP - 42 JF - Phytochemistry JO - Phytochemistry VL - 71 IS - 2-3 N2 - Herpes simplex viruses (HSVs) display affinity for cell-surface heparan sulfate proteoglycans with biological relevance in virus entry. Here, we exploit an approach to inhibiting HSV infection by using a sulfated fucoidan, and a guluronic acid-rich alginate derived from Sargassum tenerrimum, mimicking the active domain of the entry receptor. These macromolecules have apparent molecular masses of 30+/-5 and 26+/-5 kDa, respectively. They and their chemically sulfated derivatives showed activity against herpes simplex virus type 1 (HSV-1). Their inhibitory concentration 50% (IC(50)) values were in the range 0.5-15 microg/ml and they lacked cytotoxicity at concentrations up to 1000 microg/ml. The anti-HSV activity increased with increasing sulfate ester content. Our results suggest the feasibility of inhibiting HSV infection by blocking viral entry with polysaccharide having specific structure. SN - 1873-3700 UR - https://www.unboundmedicine.com/medline/citation/19931103/Polysaccharides_from_Sargassum_tenerrimum:_structural_features_chemical_modification_and_anti_viral_activity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0031-9422(09)00453-1 DB - PRIME DP - Unbound Medicine ER -