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The identification of genes specific to Prevotella intermedia and Prevotella nigrescens using genomic subtractive hybridization.
Anaerobe. 2010 Jun; 16(3):265-9.A

Abstract

Prevotella intermedia and Prevotella nigrescens, which are often isolated from periodontal sites, were once considered two different genotypes of P. intermedia. Although the genomic sequence of P. intermedia was determined recently, little is known about the genetic differences between P. intermedia and P. nigrescens. The subtractive hybridization technique is a powerful method for generating a set of DNA fragments differing between two closely related bacterial strains or species. We used subtractive hybridization to identify the DNA regions specific to P. intermedia ATCC 25611 and P. nigrescens ATCC 25261. Using this method, four P. intermedia ATCC 25611-specific and three P. nigrescens ATCC 25261-specific regions were determined. From the species-specific regions, insertion sequence (IS) elements were isolated for P. intermedia. IS elements play an important role in the pathogenicity of bacteria. For the P. intermedia-specific regions, the genes adenine-specific DNA-methyltransferase and 8-amino-7-oxononanoate synthase were isolated. The P. nigrescens-specific region contained a Flavobacterium psychrophilum SprA homologue, a cell-surface protein involved in gliding motility, Prevotella melaninogenica ATCC 25845 glutathione peroxide, and Porphyromonas gingivalis ATCC 33277 leucyl-tRNA synthetase. The results demonstrate that the subtractive hybridization technique was useful for distinguishing between the two closely related species. Furthermore, this technique will contribute to our understanding of the virulence of these species.

Authors+Show Affiliations

Division of Community Oral Health Science, Kyushu Dental College, Kitakyushu 803-8580, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19931406

Citation

Masakiyo, Yoshiaki, et al. "The Identification of Genes Specific to Prevotella Intermedia and Prevotella Nigrescens Using Genomic Subtractive Hybridization." Anaerobe, vol. 16, no. 3, 2010, pp. 265-9.
Masakiyo Y, Yoshida A, Shintani Y, et al. The identification of genes specific to Prevotella intermedia and Prevotella nigrescens using genomic subtractive hybridization. Anaerobe. 2010;16(3):265-9.
Masakiyo, Y., Yoshida, A., Shintani, Y., Takahashi, Y., Ansai, T., & Takehara, T. (2010). The identification of genes specific to Prevotella intermedia and Prevotella nigrescens using genomic subtractive hybridization. Anaerobe, 16(3), 265-9. https://doi.org/10.1016/j.anaerobe.2009.11.003
Masakiyo Y, et al. The Identification of Genes Specific to Prevotella Intermedia and Prevotella Nigrescens Using Genomic Subtractive Hybridization. Anaerobe. 2010;16(3):265-9. PubMed PMID: 19931406.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The identification of genes specific to Prevotella intermedia and Prevotella nigrescens using genomic subtractive hybridization. AU - Masakiyo,Yoshiaki, AU - Yoshida,Akihiro, AU - Shintani,Yasuyuki, AU - Takahashi,Yusuke, AU - Ansai,Toshihiro, AU - Takehara,Tadamichi, Y1 - 2009/11/18/ PY - 2008/10/23/received PY - 2009/08/21/revised PY - 2009/11/12/accepted PY - 2009/11/26/entrez PY - 2009/11/26/pubmed PY - 2010/9/23/medline SP - 265 EP - 9 JF - Anaerobe JO - Anaerobe VL - 16 IS - 3 N2 - Prevotella intermedia and Prevotella nigrescens, which are often isolated from periodontal sites, were once considered two different genotypes of P. intermedia. Although the genomic sequence of P. intermedia was determined recently, little is known about the genetic differences between P. intermedia and P. nigrescens. The subtractive hybridization technique is a powerful method for generating a set of DNA fragments differing between two closely related bacterial strains or species. We used subtractive hybridization to identify the DNA regions specific to P. intermedia ATCC 25611 and P. nigrescens ATCC 25261. Using this method, four P. intermedia ATCC 25611-specific and three P. nigrescens ATCC 25261-specific regions were determined. From the species-specific regions, insertion sequence (IS) elements were isolated for P. intermedia. IS elements play an important role in the pathogenicity of bacteria. For the P. intermedia-specific regions, the genes adenine-specific DNA-methyltransferase and 8-amino-7-oxononanoate synthase were isolated. The P. nigrescens-specific region contained a Flavobacterium psychrophilum SprA homologue, a cell-surface protein involved in gliding motility, Prevotella melaninogenica ATCC 25845 glutathione peroxide, and Porphyromonas gingivalis ATCC 33277 leucyl-tRNA synthetase. The results demonstrate that the subtractive hybridization technique was useful for distinguishing between the two closely related species. Furthermore, this technique will contribute to our understanding of the virulence of these species. SN - 1095-8274 UR - https://www.unboundmedicine.com/medline/citation/19931406/The_identification_of_genes_specific_to_Prevotella_intermedia_and_Prevotella_nigrescens_using_genomic_subtractive_hybridization_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1075-9964(09)00168-1 DB - PRIME DP - Unbound Medicine ER -