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Role of nitric oxide in the rat hippocampal CA1 in morphine antinociception.
Brain Res. 2010 Feb 08; 1313:79-88.BR

Abstract

In the present study, the effects of intra-hippocampal CA1 injections of l-arginine, a nitric oxide (NO) precursor and N(G)-nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor, on morphine-induced antinociception in rat formalin test were investigated. To induce inflammation pain, formalin (50 microl at 2.5%) was injected into the right hind-paw of male Wistar rats prior to testing. Morphine (3-9 mg/kg) was injected intraperitoneally (i.p.) 10 min before injection of formalin. The present study shows that administration of L-arginine (0.08, 0.15, 0.3, 1.0 and 3.0 microg/rat), but not L-NAME (0.15, 0.3 and 1.0 microg/rat), 5 min before formalin injection reversed morphine-induced antinociception at the early phase of formalin test. However, both drugs blocked morphine antinociception at the late phase of the test, but none of these drugs elicited any response by themselves at the tonic phase when injected alone. Moreover, the response to l-arginine was potentiated by L-NAME pre-treatment. It should be noted that a single injection of both L-arginine and L-NAME showed nociceptive effect at the early phase of the test. The present study reveals an expression of NADPH-diaphorase in the rat brain samples administered by L-arginine. Expression of NADPH-d is decreased in the samples which were pre-injected with L-NAME. This study suggests NO participation in the rat hippocampal CA1 area in morphine-induced antinociception.

Authors+Show Affiliations

Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19931515

Citation

Hashemi, Mahboobeh, et al. "Role of Nitric Oxide in the Rat Hippocampal CA1 in Morphine Antinociception." Brain Research, vol. 1313, 2010, pp. 79-88.
Hashemi M, Karami M, Zarrindast MR, et al. Role of nitric oxide in the rat hippocampal CA1 in morphine antinociception. Brain Res. 2010;1313:79-88.
Hashemi, M., Karami, M., Zarrindast, M. R., & Sahebgharani, M. (2010). Role of nitric oxide in the rat hippocampal CA1 in morphine antinociception. Brain Research, 1313, 79-88. https://doi.org/10.1016/j.brainres.2009.11.020
Hashemi M, et al. Role of Nitric Oxide in the Rat Hippocampal CA1 in Morphine Antinociception. Brain Res. 2010 Feb 8;1313:79-88. PubMed PMID: 19931515.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of nitric oxide in the rat hippocampal CA1 in morphine antinociception. AU - Hashemi,Mahboobeh, AU - Karami,Manizheh, AU - Zarrindast,Mohammad Reza, AU - Sahebgharani,Moosa, Y1 - 2009/11/18/ PY - 2009/03/18/received PY - 2009/11/08/revised PY - 2009/11/09/accepted PY - 2009/11/26/entrez PY - 2009/11/26/pubmed PY - 2010/4/7/medline SP - 79 EP - 88 JF - Brain research JO - Brain Res VL - 1313 N2 - In the present study, the effects of intra-hippocampal CA1 injections of l-arginine, a nitric oxide (NO) precursor and N(G)-nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor, on morphine-induced antinociception in rat formalin test were investigated. To induce inflammation pain, formalin (50 microl at 2.5%) was injected into the right hind-paw of male Wistar rats prior to testing. Morphine (3-9 mg/kg) was injected intraperitoneally (i.p.) 10 min before injection of formalin. The present study shows that administration of L-arginine (0.08, 0.15, 0.3, 1.0 and 3.0 microg/rat), but not L-NAME (0.15, 0.3 and 1.0 microg/rat), 5 min before formalin injection reversed morphine-induced antinociception at the early phase of formalin test. However, both drugs blocked morphine antinociception at the late phase of the test, but none of these drugs elicited any response by themselves at the tonic phase when injected alone. Moreover, the response to l-arginine was potentiated by L-NAME pre-treatment. It should be noted that a single injection of both L-arginine and L-NAME showed nociceptive effect at the early phase of the test. The present study reveals an expression of NADPH-diaphorase in the rat brain samples administered by L-arginine. Expression of NADPH-d is decreased in the samples which were pre-injected with L-NAME. This study suggests NO participation in the rat hippocampal CA1 area in morphine-induced antinociception. SN - 1872-6240 UR - https://www.unboundmedicine.com/medline/citation/19931515/Role_of_nitric_oxide_in_the_rat_hippocampal_CA1_in_morphine_antinociception_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(09)02446-9 DB - PRIME DP - Unbound Medicine ER -