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Anti-inflammatory, anticholinesterase, antioxidant and phytochemical properties of medicinal plants used for pain-related ailments in South Africa.
J Ethnopharmacol. 2010 Feb 03; 127(2):235-41.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Extracts of seven South African medicinal plants used traditionally for the treatment of pain-related ailments were evaluated.

AIMS OF THE STUDY

The study was aimed at evaluating medicinal and therapeutic potentials of the investigated traditional medicinal plants. Plant extracts were evaluated for anti-inflammatory activity and other pharmacological properties such as anticholinesterase and antioxidant activities. Phytochemical analysis of total phenolic contents, condensed tannins, gallotannins and flavonoids in the aqueous methanol extracts of the medicinal plants were also carried out.

MATERIALS AND METHODS

The evaluation of anti-inflammatory activity of 50% methanol (50% MeOH), petroleum ether (PE), dichloromethane (DCM) and ethanol (EtOH) plant extracts was done against cyclooxygenase-1 and -2 (COX-1 and COX-2) enzymes. 50% MeOH, PE, DCM and EtOH extracts were tested for acetylcholinesterase (AChE) inhibition, while 50% MeOH extracts were tested for 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity and ferric-reducing power in the antioxidant assays. Total phenolic compounds, condensed tannins, gallotannins and flavonoids were quantitatively determined using spectrophotometric methods.

RESULTS

At the screening assay concentration (0.25 microg/microl), 13 extracts showed good COX-1 inhibitory activity (>50%), while good activity was observed in 15 extracts against COX-2 enzyme. All the extracts of Crinum moorei (bulbs) showed good inhibition against both COX-1 and COX-2 enzymes. Though not significantly different (P=0.05), the highest COX-1 percentage inhibition (100%) was shown by Aloe ferox leaf PE and Colocasia antiquorum tuber DCM extracts, while Colocasia antiquorum tuber PE extract exhibited the highest (92.7%) percentage inhibition against COX-2. Crinum moorei bulb DCM extract showed the lowest EC(50) value (2.9 microg/ml) in the AChE assay. In addition, good to moderate bioactivities were observed in some extracts of Aloe ferox (leaves), Crinum moorei (bulbs) and Pycnostachys reticulata (leaves) in all the assays. The presence and/or amounts of phenolic compounds varied with plant species.

CONCLUSION

The results obtained in this study validate the use of the investigated medicinal plants in South African traditional medicine for pain-related ailments.

Authors+Show Affiliations

Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19932161

Citation

Fawole, O A., et al. "Anti-inflammatory, Anticholinesterase, Antioxidant and Phytochemical Properties of Medicinal Plants Used for Pain-related Ailments in South Africa." Journal of Ethnopharmacology, vol. 127, no. 2, 2010, pp. 235-41.
Fawole OA, Amoo SO, Ndhlala AR, et al. Anti-inflammatory, anticholinesterase, antioxidant and phytochemical properties of medicinal plants used for pain-related ailments in South Africa. J Ethnopharmacol. 2010;127(2):235-41.
Fawole, O. A., Amoo, S. O., Ndhlala, A. R., Light, M. E., Finnie, J. F., & Van Staden, J. (2010). Anti-inflammatory, anticholinesterase, antioxidant and phytochemical properties of medicinal plants used for pain-related ailments in South Africa. Journal of Ethnopharmacology, 127(2), 235-41. https://doi.org/10.1016/j.jep.2009.11.015
Fawole OA, et al. Anti-inflammatory, Anticholinesterase, Antioxidant and Phytochemical Properties of Medicinal Plants Used for Pain-related Ailments in South Africa. J Ethnopharmacol. 2010 Feb 3;127(2):235-41. PubMed PMID: 19932161.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-inflammatory, anticholinesterase, antioxidant and phytochemical properties of medicinal plants used for pain-related ailments in South Africa. AU - Fawole,O A, AU - Amoo,S O, AU - Ndhlala,A R, AU - Light,M E, AU - Finnie,J F, AU - Van Staden,J, Y1 - 2009/11/20/ PY - 2009/09/29/received PY - 2009/11/12/revised PY - 2009/11/13/accepted PY - 2009/11/26/entrez PY - 2009/11/26/pubmed PY - 2010/9/9/medline SP - 235 EP - 41 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 127 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Extracts of seven South African medicinal plants used traditionally for the treatment of pain-related ailments were evaluated. AIMS OF THE STUDY: The study was aimed at evaluating medicinal and therapeutic potentials of the investigated traditional medicinal plants. Plant extracts were evaluated for anti-inflammatory activity and other pharmacological properties such as anticholinesterase and antioxidant activities. Phytochemical analysis of total phenolic contents, condensed tannins, gallotannins and flavonoids in the aqueous methanol extracts of the medicinal plants were also carried out. MATERIALS AND METHODS: The evaluation of anti-inflammatory activity of 50% methanol (50% MeOH), petroleum ether (PE), dichloromethane (DCM) and ethanol (EtOH) plant extracts was done against cyclooxygenase-1 and -2 (COX-1 and COX-2) enzymes. 50% MeOH, PE, DCM and EtOH extracts were tested for acetylcholinesterase (AChE) inhibition, while 50% MeOH extracts were tested for 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity and ferric-reducing power in the antioxidant assays. Total phenolic compounds, condensed tannins, gallotannins and flavonoids were quantitatively determined using spectrophotometric methods. RESULTS: At the screening assay concentration (0.25 microg/microl), 13 extracts showed good COX-1 inhibitory activity (>50%), while good activity was observed in 15 extracts against COX-2 enzyme. All the extracts of Crinum moorei (bulbs) showed good inhibition against both COX-1 and COX-2 enzymes. Though not significantly different (P=0.05), the highest COX-1 percentage inhibition (100%) was shown by Aloe ferox leaf PE and Colocasia antiquorum tuber DCM extracts, while Colocasia antiquorum tuber PE extract exhibited the highest (92.7%) percentage inhibition against COX-2. Crinum moorei bulb DCM extract showed the lowest EC(50) value (2.9 microg/ml) in the AChE assay. In addition, good to moderate bioactivities were observed in some extracts of Aloe ferox (leaves), Crinum moorei (bulbs) and Pycnostachys reticulata (leaves) in all the assays. The presence and/or amounts of phenolic compounds varied with plant species. CONCLUSION: The results obtained in this study validate the use of the investigated medicinal plants in South African traditional medicine for pain-related ailments. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/19932161/Anti_inflammatory_anticholinesterase_antioxidant_and_phytochemical_properties_of_medicinal_plants_used_for_pain_related_ailments_in_South_Africa_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(09)00722-3 DB - PRIME DP - Unbound Medicine ER -