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Innovative development and validation of an HPLC/DAD method for the qualitative and quantitative determination of major cannabinoids in cannabis plant material.

Abstract

GC is commonly used for the analysis of cannabis samples, e.g. in forensic chemistry. However, as this method is based on heating of the sample, acidic forms of cannabinoids are decarboxylated into their neutral counterparts. Conversely, HPLC permits the determination of the original composition of plant cannabinoids by direct analysis. Several HPLC methods have been described in the literature, but most of them failed to separate efficiently all the cannabinoids or were not validated according to general guidelines. By use of an innovative methodology for modelling chromatographic responses, a simple and accurate HPLC/DAD method was developed for the quantification of major neutral and acidic cannabinoids present in cannabis plant material: Delta9-tetrahydrocannabinol (THC), THC acid (THCA), cannabidiol (CBD), CBD acid (CBDA), cannabigerol (CBG), CBG acid (CBGA) and cannabinol (CBN). Delta8-Tetrahydrocannabinol (Delta8-THC) was determined qualitatively. Following the practice of design of experiments, predictive multilinear models were developed and used in order to find optimal chromatographic analytical conditions. The method was validated following an approach using accuracy profiles based on beta-expectation tolerance intervals for the total error measurement, and assessing the measurements uncertainty. This analytical method can be used for diverse applications, e.g. plant phenotype determination, evaluation of psychoactive potency and control of material quality.

Authors+Show Affiliations

Laboratory of Clinical, Forensic and Environmental Toxicology, CIRM, CHU Sart-Tilman, University of Liège, B-4000 Liège, Belgium. b.debacker@ulg.ac.beNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Studies

Language

eng

PubMed ID

19932642

Citation

De Backer, Benjamin, et al. "Innovative Development and Validation of an HPLC/DAD Method for the Qualitative and Quantitative Determination of Major Cannabinoids in Cannabis Plant Material." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 877, no. 32, 2009, pp. 4115-24.
De Backer B, Debrus B, Lebrun P, et al. Innovative development and validation of an HPLC/DAD method for the qualitative and quantitative determination of major cannabinoids in cannabis plant material. J Chromatogr B Analyt Technol Biomed Life Sci. 2009;877(32):4115-24.
De Backer, B., Debrus, B., Lebrun, P., Theunis, L., Dubois, N., Decock, L., ... Charlier, C. (2009). Innovative development and validation of an HPLC/DAD method for the qualitative and quantitative determination of major cannabinoids in cannabis plant material. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 877(32), pp. 4115-24. doi:10.1016/j.jchromb.2009.11.004.
De Backer B, et al. Innovative Development and Validation of an HPLC/DAD Method for the Qualitative and Quantitative Determination of Major Cannabinoids in Cannabis Plant Material. J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Dec 15;877(32):4115-24. PubMed PMID: 19932642.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Innovative development and validation of an HPLC/DAD method for the qualitative and quantitative determination of major cannabinoids in cannabis plant material. AU - De Backer,Benjamin, AU - Debrus,Benjamin, AU - Lebrun,Pierre, AU - Theunis,Laetitia, AU - Dubois,Nathalie, AU - Decock,Lies, AU - Verstraete,Alain, AU - Hubert,Philippe, AU - Charlier,Corinne, Y1 - 2009/11/06/ PY - 2009/08/28/received PY - 2009/10/28/revised PY - 2009/11/01/accepted PY - 2009/11/26/entrez PY - 2009/11/26/pubmed PY - 2010/2/6/medline SP - 4115 EP - 24 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 877 IS - 32 N2 - GC is commonly used for the analysis of cannabis samples, e.g. in forensic chemistry. However, as this method is based on heating of the sample, acidic forms of cannabinoids are decarboxylated into their neutral counterparts. Conversely, HPLC permits the determination of the original composition of plant cannabinoids by direct analysis. Several HPLC methods have been described in the literature, but most of them failed to separate efficiently all the cannabinoids or were not validated according to general guidelines. By use of an innovative methodology for modelling chromatographic responses, a simple and accurate HPLC/DAD method was developed for the quantification of major neutral and acidic cannabinoids present in cannabis plant material: Delta9-tetrahydrocannabinol (THC), THC acid (THCA), cannabidiol (CBD), CBD acid (CBDA), cannabigerol (CBG), CBG acid (CBGA) and cannabinol (CBN). Delta8-Tetrahydrocannabinol (Delta8-THC) was determined qualitatively. Following the practice of design of experiments, predictive multilinear models were developed and used in order to find optimal chromatographic analytical conditions. The method was validated following an approach using accuracy profiles based on beta-expectation tolerance intervals for the total error measurement, and assessing the measurements uncertainty. This analytical method can be used for diverse applications, e.g. plant phenotype determination, evaluation of psychoactive potency and control of material quality. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/19932642/Innovative_development_and_validation_of_an_HPLC/DAD_method_for_the_qualitative_and_quantitative_determination_of_major_cannabinoids_in_cannabis_plant_material_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(09)00754-5 DB - PRIME DP - Unbound Medicine ER -