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The maternal homocysteine pathway is influenced by riboflavin intake and MTHFR polymorphisms without affecting the risk of orofacial clefts in the offspring.
Eur J Clin Nutr 2010; 64(3):266-73EJ

Abstract

BACKGROUND/OBJECTIVES

Riboflavin is a cofactor for the 5,10-methylenetetrahydrofolate reductase (MTHFR) enzyme involved in the homocysteine pathway. The aim of this study was to investigate the effects of maternal riboflavin intake and two MTHFR polymorphisms (677C>T; Ala222Val and 1298A>C; Glu429Ala substitutions) on the biomarkers of the homocysteine pathway, and investigate the risk of having offspring with an orofacial cleft (OFC).

SUBJECTS/METHODS

In a case-control study design, dietary riboflavin intake and the MTHFR 677C>T and 1298A>C polymorphisms were evaluated in 123 OFC and 108 control mothers by using food frequency questionnaires and blood samples. Homocysteine (tHcy), folate and vitamin B12 concentrations in blood were analyzed in 70 cases and 68 controls. Linear and logistic regression analyses were applied.

RESULTS

At 14 months postpartum riboflavin intake and MTHFR 677C>T and 1298A>C genotypes were not significantly different between cases and controls. The 677TT genotype showed lower folate concentrations compared to C-allele carriers with a mean difference of 2.8 nmol/l in serum and 174 nmol/l in red blood cell (both P's=0.01). Every mg per day increase of dietary riboflavin intake was positively associated with increase in vitamin B12 concentration by 52.1% (P<0.01). This effect was most pronounced in MTHFR 677TT homozygotes (205.1%, P=0.03). The riboflavin-adjusted MTHFR 677TT and 1298CC genotypes showed a trend toward an increasing risk for OFC, adjusted odds ratio 1.7 (confidence interval (95% CI), 0.7-4.5) and 1.6 (95% CI, 0.7-4.2), respectively.

CONCLUSIONS

Maternal riboflavin intake is significantly associated with biomarkers of the homocysteine pathway, with the strongest effects in MTHFR 677TT homozygotes. The maternal risk of having OFC offspring, however, is not associated with dietary riboflavin intake.

Authors+Show Affiliations

Department of Obstetrics and Gynaecology, Erasmus University Medical Centre, Rotterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19935819

Citation

Vujkovic, M, et al. "The Maternal Homocysteine Pathway Is Influenced By Riboflavin Intake and MTHFR Polymorphisms Without Affecting the Risk of Orofacial Clefts in the Offspring." European Journal of Clinical Nutrition, vol. 64, no. 3, 2010, pp. 266-73.
Vujkovic M, Steegers EA, van Meurs J, et al. The maternal homocysteine pathway is influenced by riboflavin intake and MTHFR polymorphisms without affecting the risk of orofacial clefts in the offspring. Eur J Clin Nutr. 2010;64(3):266-73.
Vujkovic, M., Steegers, E. A., van Meurs, J., Yazdanpanah, N., van Rooij, I. A., Uitterlinden, A. G., & Steegers-Theunissen, R. P. (2010). The maternal homocysteine pathway is influenced by riboflavin intake and MTHFR polymorphisms without affecting the risk of orofacial clefts in the offspring. European Journal of Clinical Nutrition, 64(3), pp. 266-73. doi:10.1038/ejcn.2009.138.
Vujkovic M, et al. The Maternal Homocysteine Pathway Is Influenced By Riboflavin Intake and MTHFR Polymorphisms Without Affecting the Risk of Orofacial Clefts in the Offspring. Eur J Clin Nutr. 2010;64(3):266-73. PubMed PMID: 19935819.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The maternal homocysteine pathway is influenced by riboflavin intake and MTHFR polymorphisms without affecting the risk of orofacial clefts in the offspring. AU - Vujkovic,M, AU - Steegers,E A, AU - van Meurs,J, AU - Yazdanpanah,N, AU - van Rooij,I A, AU - Uitterlinden,A G, AU - Steegers-Theunissen,R P, Y1 - 2009/11/25/ PY - 2009/11/26/entrez PY - 2009/11/26/pubmed PY - 2010/5/15/medline SP - 266 EP - 73 JF - European journal of clinical nutrition JO - Eur J Clin Nutr VL - 64 IS - 3 N2 - BACKGROUND/OBJECTIVES: Riboflavin is a cofactor for the 5,10-methylenetetrahydrofolate reductase (MTHFR) enzyme involved in the homocysteine pathway. The aim of this study was to investigate the effects of maternal riboflavin intake and two MTHFR polymorphisms (677C>T; Ala222Val and 1298A>C; Glu429Ala substitutions) on the biomarkers of the homocysteine pathway, and investigate the risk of having offspring with an orofacial cleft (OFC). SUBJECTS/METHODS: In a case-control study design, dietary riboflavin intake and the MTHFR 677C>T and 1298A>C polymorphisms were evaluated in 123 OFC and 108 control mothers by using food frequency questionnaires and blood samples. Homocysteine (tHcy), folate and vitamin B12 concentrations in blood were analyzed in 70 cases and 68 controls. Linear and logistic regression analyses were applied. RESULTS: At 14 months postpartum riboflavin intake and MTHFR 677C>T and 1298A>C genotypes were not significantly different between cases and controls. The 677TT genotype showed lower folate concentrations compared to C-allele carriers with a mean difference of 2.8 nmol/l in serum and 174 nmol/l in red blood cell (both P's=0.01). Every mg per day increase of dietary riboflavin intake was positively associated with increase in vitamin B12 concentration by 52.1% (P<0.01). This effect was most pronounced in MTHFR 677TT homozygotes (205.1%, P=0.03). The riboflavin-adjusted MTHFR 677TT and 1298CC genotypes showed a trend toward an increasing risk for OFC, adjusted odds ratio 1.7 (confidence interval (95% CI), 0.7-4.5) and 1.6 (95% CI, 0.7-4.2), respectively. CONCLUSIONS: Maternal riboflavin intake is significantly associated with biomarkers of the homocysteine pathway, with the strongest effects in MTHFR 677TT homozygotes. The maternal risk of having OFC offspring, however, is not associated with dietary riboflavin intake. SN - 1476-5640 UR - https://www.unboundmedicine.com/medline/citation/19935819/The_maternal_homocysteine_pathway_is_influenced_by_riboflavin_intake_and_MTHFR_polymorphisms_without_affecting_the_risk_of_orofacial_clefts_in_the_offspring_ L2 - http://dx.doi.org/10.1038/ejcn.2009.138 DB - PRIME DP - Unbound Medicine ER -