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Measurement of caffeic and ferulic acid equivalents in plasma after coffee consumption: small intestine and colon are key sites for coffee metabolism.
Mol Nutr Food Res. 2010 Jun; 54(6):760-6.MN

Abstract

Previous studies on coffee examined absorption of phenolic acids (PA) in the small intestine, but not the contribution of the colon to absorption. Nine healthy volunteers ingested instant soluble coffee (approximately 335 mg total chlorogenic acids (CGAs)) in water. Blood samples were taken over 12 h, and at 24 h to assess return to baseline. Many previous studies, which used glucuronidase and sulfatase, measured only PA and did not rigorously assess CGAs. To improve this, plasma samples were analyzed after full hydrolysis by chlorogenate esterase, glucuronidase and sulfatase to release aglycone equivalents of PA followed by liquid-liquid extraction and ESI-LC-ESI-MS/MS detection. Ferulic, caffeic and isoferulic acid equivalents appeared rapidly in plasma, peaking at 1-2 h. Dihydrocaffeic and dihydroferulic acids appeared in plasma 6-8 h after ingestion (T(max=)8-12 h). Substantial variability in maximum plasma concentration and T(max) was also observed between individuals. This study confirms that the small intestine is a significant site for absorption of PA, but shows for the first time that the colon/microflora play the major role in absorption and metabolism of CGAs and PA from coffee.

Authors+Show Affiliations

Nestlé Research Center, Nestec Limited, Vers-Chez-Les-Blanc, Lausanne, Switzerland. mathieu.renouf@rdls.nestle.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19937852

Citation

Renouf, Mathieu, et al. "Measurement of Caffeic and Ferulic Acid Equivalents in Plasma After Coffee Consumption: Small Intestine and Colon Are Key Sites for Coffee Metabolism." Molecular Nutrition & Food Research, vol. 54, no. 6, 2010, pp. 760-6.
Renouf M, Guy PA, Marmet C, et al. Measurement of caffeic and ferulic acid equivalents in plasma after coffee consumption: small intestine and colon are key sites for coffee metabolism. Mol Nutr Food Res. 2010;54(6):760-6.
Renouf, M., Guy, P. A., Marmet, C., Fraering, A. L., Longet, K., Moulin, J., Enslen, M., Barron, D., Dionisi, F., Cavin, C., Williamson, G., & Steiling, H. (2010). Measurement of caffeic and ferulic acid equivalents in plasma after coffee consumption: small intestine and colon are key sites for coffee metabolism. Molecular Nutrition & Food Research, 54(6), 760-6. https://doi.org/10.1002/mnfr.200900056
Renouf M, et al. Measurement of Caffeic and Ferulic Acid Equivalents in Plasma After Coffee Consumption: Small Intestine and Colon Are Key Sites for Coffee Metabolism. Mol Nutr Food Res. 2010;54(6):760-6. PubMed PMID: 19937852.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Measurement of caffeic and ferulic acid equivalents in plasma after coffee consumption: small intestine and colon are key sites for coffee metabolism. AU - Renouf,Mathieu, AU - Guy,Philippe A, AU - Marmet,Cynthia, AU - Fraering,Anne-Lise, AU - Longet,Karin, AU - Moulin,Julie, AU - Enslen,Marc, AU - Barron,Denis, AU - Dionisi,Fabiola, AU - Cavin,Christophe, AU - Williamson,Gary, AU - Steiling,Heike, PY - 2009/11/26/entrez PY - 2009/11/26/pubmed PY - 2010/9/18/medline SP - 760 EP - 6 JF - Molecular nutrition & food research JO - Mol Nutr Food Res VL - 54 IS - 6 N2 - Previous studies on coffee examined absorption of phenolic acids (PA) in the small intestine, but not the contribution of the colon to absorption. Nine healthy volunteers ingested instant soluble coffee (approximately 335 mg total chlorogenic acids (CGAs)) in water. Blood samples were taken over 12 h, and at 24 h to assess return to baseline. Many previous studies, which used glucuronidase and sulfatase, measured only PA and did not rigorously assess CGAs. To improve this, plasma samples were analyzed after full hydrolysis by chlorogenate esterase, glucuronidase and sulfatase to release aglycone equivalents of PA followed by liquid-liquid extraction and ESI-LC-ESI-MS/MS detection. Ferulic, caffeic and isoferulic acid equivalents appeared rapidly in plasma, peaking at 1-2 h. Dihydrocaffeic and dihydroferulic acids appeared in plasma 6-8 h after ingestion (T(max=)8-12 h). Substantial variability in maximum plasma concentration and T(max) was also observed between individuals. This study confirms that the small intestine is a significant site for absorption of PA, but shows for the first time that the colon/microflora play the major role in absorption and metabolism of CGAs and PA from coffee. SN - 1613-4133 UR - https://www.unboundmedicine.com/medline/citation/19937852/Measurement_of_caffeic_and_ferulic_acid_equivalents_in_plasma_after_coffee_consumption:_small_intestine_and_colon_are_key_sites_for_coffee_metabolism_ L2 - https://doi.org/10.1002/mnfr.200900056 DB - PRIME DP - Unbound Medicine ER -