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Absorption, metabolism, and excretion of green tea flavan-3-ols in humans with an ileostomy.
Mol Nutr Food Res. 2010 Mar; 54(3):323-34.MN

Abstract

Green tea containing 634 micromol of flavan-3-ols was ingested by human subjects with an ileostomy. Ileal fluid, plasma, and urine collected 0-24 h after ingestion were analysed by HPLC-MS. The ileal fluid contained 70% of the ingested flavan-3-ols in the form of parent compounds (33%) and 23 metabolites (37%). The main metabolites effluxed back into the lumen of the small intestine were O-linked sulphates and methyl-sulphates of (epi)catechin and (epi)gallocatechin. Thus, in subjects with a functioning colon substantial quantities of flavan-3-ols would pass from the small to the large intestine. Plasma contained 16 metabolites, principally methylated, sulphated, and glucuronidated conjugates of (epi)catechin and (epi)gallocatechin, exhibiting 101-256 nM peak plasma concentration and the time to reach peak plasma concentration ranging from 0.8 to 2.2 h. Plasma pharmacokinetic profiles were similar to those obtained with healthy subjects, indicating that flavan-3-ol absorption occurs in the small intestine. Ileostomists had earlier plasma time to reach peak plasma concentration values than subjects with an intact colon, indicating the absence of an ileal brake. Urine contained 18 metabolites of (epi)catechin and (epi)gallocatechin in amounts corresponding to 6.8+/-0.6% of total flavan-3-ol intake. However, excretion of (epi)catechin metabolites was equivalent to 27% of the ingested (-)-epicatechin and (+)-catechin.

Authors+Show Affiliations

Division of Environmental and Evolutionary Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19937856

Citation

Stalmach, Angélique, et al. "Absorption, Metabolism, and Excretion of Green Tea Flavan-3-ols in Humans With an Ileostomy." Molecular Nutrition & Food Research, vol. 54, no. 3, 2010, pp. 323-34.
Stalmach A, Mullen W, Steiling H, et al. Absorption, metabolism, and excretion of green tea flavan-3-ols in humans with an ileostomy. Mol Nutr Food Res. 2010;54(3):323-34.
Stalmach, A., Mullen, W., Steiling, H., Williamson, G., Lean, M. E., & Crozier, A. (2010). Absorption, metabolism, and excretion of green tea flavan-3-ols in humans with an ileostomy. Molecular Nutrition & Food Research, 54(3), 323-34. https://doi.org/10.1002/mnfr.200900194
Stalmach A, et al. Absorption, Metabolism, and Excretion of Green Tea Flavan-3-ols in Humans With an Ileostomy. Mol Nutr Food Res. 2010;54(3):323-34. PubMed PMID: 19937856.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Absorption, metabolism, and excretion of green tea flavan-3-ols in humans with an ileostomy. AU - Stalmach,Angélique, AU - Mullen,William, AU - Steiling,Heike, AU - Williamson,Gary, AU - Lean,Michael E J, AU - Crozier,Alan, PY - 2009/11/26/entrez PY - 2009/11/26/pubmed PY - 2010/7/9/medline SP - 323 EP - 34 JF - Molecular nutrition & food research JO - Mol Nutr Food Res VL - 54 IS - 3 N2 - Green tea containing 634 micromol of flavan-3-ols was ingested by human subjects with an ileostomy. Ileal fluid, plasma, and urine collected 0-24 h after ingestion were analysed by HPLC-MS. The ileal fluid contained 70% of the ingested flavan-3-ols in the form of parent compounds (33%) and 23 metabolites (37%). The main metabolites effluxed back into the lumen of the small intestine were O-linked sulphates and methyl-sulphates of (epi)catechin and (epi)gallocatechin. Thus, in subjects with a functioning colon substantial quantities of flavan-3-ols would pass from the small to the large intestine. Plasma contained 16 metabolites, principally methylated, sulphated, and glucuronidated conjugates of (epi)catechin and (epi)gallocatechin, exhibiting 101-256 nM peak plasma concentration and the time to reach peak plasma concentration ranging from 0.8 to 2.2 h. Plasma pharmacokinetic profiles were similar to those obtained with healthy subjects, indicating that flavan-3-ol absorption occurs in the small intestine. Ileostomists had earlier plasma time to reach peak plasma concentration values than subjects with an intact colon, indicating the absence of an ileal brake. Urine contained 18 metabolites of (epi)catechin and (epi)gallocatechin in amounts corresponding to 6.8+/-0.6% of total flavan-3-ol intake. However, excretion of (epi)catechin metabolites was equivalent to 27% of the ingested (-)-epicatechin and (+)-catechin. SN - 1613-4133 UR - https://www.unboundmedicine.com/medline/citation/19937856/Absorption_metabolism_and_excretion_of_green_tea_flavan_3_ols_in_humans_with_an_ileostomy_ L2 - https://doi.org/10.1002/mnfr.200900194 DB - PRIME DP - Unbound Medicine ER -