Tags

Type your tag names separated by a space and hit enter

Human sperm anatomy: ultrastructural localization of the cannabinoid1 receptor and a potential role of anandamide in sperm survival and acrosome reaction.
Anat Rec (Hoboken). 2010 Feb; 293(2):298-309.AR

Abstract

Recently, the endocannabinoid (EC) system and the presence of CB1 receptor (CB1-R), have been identified in human sperm. However, the effects of EC receptor ligands such as anandamide (N-arachidonoylethanolamine) and the role of EC system in male fertility is still largely unexplored. In the present study, we investigated the ultrastructural compartmentalization of CB1-R and analyzed the effects of its stimulation by using a stable analog of anandamide, 2-methylarachidonyl-2'-fluoro-ethylamide (MET-F-AEA). We focused particularly on sperm survival and acrosin activity. The study of human sperm anatomy by transmission electron microscopy with immunogold analysis revealed the location of the CB1-R prevalently in the sperm membranes of the head and interestingly on the mitochondria. The effect of different concentrations of MET-F-AEA from 100 nM to 1 microM evidenced a significant decrease of sperm survival. Interestingly, we analyzed this negative effect at molecular level, testing the EC action on different known sperm survival targets. MET-F-AEA-treatment decreased both pBCL2 and pAkt, two prosurvival proteins, and increased pPTEN expression which is the main regulator of the PI3K/Akt pathway. Moreover, a biphasic effect was observed with increasing MET-F-AEA concentrations on the acrosin activity. The blockage of the CB1-R by using its selective antagonist SR141716 (rimonabant) induced an opposite action on sperm survival supporting a role for this receptor in the biology of the male gamete.

Authors+Show Affiliations

Department of Pharmaco-Biology, University of Calabria, Arcavacata di Rende, Cosenza, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19938110

Citation

Aquila, Saveria, et al. "Human Sperm Anatomy: Ultrastructural Localization of the Cannabinoid1 Receptor and a Potential Role of Anandamide in Sperm Survival and Acrosome Reaction." Anatomical Record (Hoboken, N.J. : 2007), vol. 293, no. 2, 2010, pp. 298-309.
Aquila S, Guido C, Santoro A, et al. Human sperm anatomy: ultrastructural localization of the cannabinoid1 receptor and a potential role of anandamide in sperm survival and acrosome reaction. Anat Rec (Hoboken). 2010;293(2):298-309.
Aquila, S., Guido, C., Santoro, A., Perrotta, I., Laezza, C., Bifulco, M., & Sebastiano, A. (2010). Human sperm anatomy: ultrastructural localization of the cannabinoid1 receptor and a potential role of anandamide in sperm survival and acrosome reaction. Anatomical Record (Hoboken, N.J. : 2007), 293(2), 298-309. https://doi.org/10.1002/ar.21042
Aquila S, et al. Human Sperm Anatomy: Ultrastructural Localization of the Cannabinoid1 Receptor and a Potential Role of Anandamide in Sperm Survival and Acrosome Reaction. Anat Rec (Hoboken). 2010;293(2):298-309. PubMed PMID: 19938110.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human sperm anatomy: ultrastructural localization of the cannabinoid1 receptor and a potential role of anandamide in sperm survival and acrosome reaction. AU - Aquila,Saveria, AU - Guido,Carmela, AU - Santoro,Antonietta, AU - Perrotta,Ida, AU - Laezza,Chiara, AU - Bifulco,Maurizio, AU - Sebastiano,Andò, PY - 2009/11/26/entrez PY - 2009/11/26/pubmed PY - 2010/4/2/medline SP - 298 EP - 309 JF - Anatomical record (Hoboken, N.J. : 2007) JO - Anat Rec (Hoboken) VL - 293 IS - 2 N2 - Recently, the endocannabinoid (EC) system and the presence of CB1 receptor (CB1-R), have been identified in human sperm. However, the effects of EC receptor ligands such as anandamide (N-arachidonoylethanolamine) and the role of EC system in male fertility is still largely unexplored. In the present study, we investigated the ultrastructural compartmentalization of CB1-R and analyzed the effects of its stimulation by using a stable analog of anandamide, 2-methylarachidonyl-2'-fluoro-ethylamide (MET-F-AEA). We focused particularly on sperm survival and acrosin activity. The study of human sperm anatomy by transmission electron microscopy with immunogold analysis revealed the location of the CB1-R prevalently in the sperm membranes of the head and interestingly on the mitochondria. The effect of different concentrations of MET-F-AEA from 100 nM to 1 microM evidenced a significant decrease of sperm survival. Interestingly, we analyzed this negative effect at molecular level, testing the EC action on different known sperm survival targets. MET-F-AEA-treatment decreased both pBCL2 and pAkt, two prosurvival proteins, and increased pPTEN expression which is the main regulator of the PI3K/Akt pathway. Moreover, a biphasic effect was observed with increasing MET-F-AEA concentrations on the acrosin activity. The blockage of the CB1-R by using its selective antagonist SR141716 (rimonabant) induced an opposite action on sperm survival supporting a role for this receptor in the biology of the male gamete. SN - 1932-8494 UR - https://www.unboundmedicine.com/medline/citation/19938110/Human_sperm_anatomy:_ultrastructural_localization_of_the_cannabinoid1_receptor_and_a_potential_role_of_anandamide_in_sperm_survival_and_acrosome_reaction_ L2 - https://doi.org/10.1002/ar.21042 DB - PRIME DP - Unbound Medicine ER -