Citation
Bao, Fuxiang, et al. "[Isolation and Identification of a Human Single Chain Fv Antibody Against Amyloid-beta 1-42 Soluble Oligomers From a Human Phage Display Library]." Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, vol. 25, no. 8, 2009, pp. 1195-203.
Bao F, He J, Cao G, et al. [Isolation and identification of a human single chain Fv antibody against amyloid-beta 1-42 soluble oligomers from a human phage display library]. Sheng Wu Gong Cheng Xue Bao. 2009;25(8):1195-203.
Bao, F., He, J., Cao, G., Yin, F., Wang, X., Pang, S., Zhang, Y., & Hong, T. (2009). [Isolation and identification of a human single chain Fv antibody against amyloid-beta 1-42 soluble oligomers from a human phage display library]. Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology, 25(8), 1195-203.
Bao F, et al. [Isolation and Identification of a Human Single Chain Fv Antibody Against Amyloid-beta 1-42 Soluble Oligomers From a Human Phage Display Library]. Sheng Wu Gong Cheng Xue Bao. 2009;25(8):1195-203. PubMed PMID: 19938457.
TY - JOUR
T1 - [Isolation and identification of a human single chain Fv antibody against amyloid-beta 1-42 soluble oligomers from a human phage display library].
AU - Bao,Fuxiang,
AU - He,Jinsheng,
AU - Cao,Guifang,
AU - Yin,Fan,
AU - Wang,Xin,
AU - Pang,Siyuan,
AU - Zhang,Ying,
AU - Hong,Tao,
PY - 2009/11/27/entrez
PY - 2009/11/27/pubmed
PY - 2011/4/29/medline
SP - 1195
EP - 203
JF - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JO - Sheng Wu Gong Cheng Xue Bao
VL - 25
IS - 8
N2 - To get specific scFv (Single-chain fragment variable) antibody against soluble Abeta1-42(Amyloid-beta) oligomers, we constructed a human single-chain Fv (scFv) antibody library by phage display technology. Using RT-PCR, we amplified the variable heavy (VH) and variable light (VL) genes from peripheral blood lymphocytes (PBL). Then we obtained the scFv fragments through SOE-PCR, and the scFv fragments were cloned into the vector pCANTAB5E and electroporated into competent Escherichia coli TG1 cells. Consequently, a scFv phage display library containing 2.5 x 10(9) clones was constructed. The recombinant phagemids were rescued by reinfection of helper phage M13K07. Recombinant phages specific for Abeta1-42 oligomers were enriched after four rounds of biopanning and the antigen-positive clones were selected from the enriched clones by phage ELISA. Positive clone B19 was used to infect E. coli HB2151 to express soluble scFv antibody. SDS-PAGE and Western blotting analysis showed that the soluble scFv B19 antibody was expressed successfully and could bind specifically to Abeta1-42 trimer and protofiber. The specific scFv against Abeta1-42 oligomers can be used in the therapeutic research on Alzheimer's disease.
SN - 1000-3061
UR - https://www.unboundmedicine.com/medline/citation/19938457/[Isolation_and_identification_of_a_human_single_chain_Fv_antibody_against_amyloid_beta_1_42_soluble_oligomers_from_a_human_phage_display_library]_
L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:19938457
DB - PRIME
DP - Unbound Medicine
ER -