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Overexpression of serine acetlytransferase produced large increases in O-acetylserine and free cysteine in developing seeds of a grain legume.
J Exp Bot 2010; 61(3):721-33JE

Abstract

There have been many attempts to increase concentrations of the nutritionally essential sulphur amino acids by modifying their biosynthetic pathway in leaves of transgenic plants. This report describes the first modification of cysteine biosynthesis in developing seeds; those of the grain legume, narrow leaf lupin (Lupinus angustifolius, L.). Expression in developing lupin embryos of a serine acetyltransferase (SAT) from Arabidopsis thaliana (AtSAT1 or AtSerat 2;1) was associated with increases of up to 5-fold in the concentrations of O-acetylserine (OAS), the immediate product of SAT, and up to 26-fold in free cysteine, resulting in some of the highest in vivo concentrations of these metabolites yet reported. Despite the dramatic changes in free cysteine in developing embryos of SAT overexpressers, concentrations of free methionine in developing embryos, and the total cysteine and methionine concentrations in mature seeds were not significantly altered. Pooled F(2) seeds segregating for the SAT transgene and for a transgene encoding a methionine- and cysteine-rich sunflower seed storage protein also had increased OAS and free cysteine, but not free methionine, during development, and no increase in mature seed total sulphur amino acids compared with controls lacking SAT overexpression. The data support the view that the cysteine biosynthetic pathway is active in developing seeds, and indicate that SAT activity limits cysteine biosynthesis, but that cysteine supply is not limiting for methionine biosynthesis or for storage protein synthesis in maturing lupin embryos in conditions of adequate sulphur nutrition. OAS and free methionine, but not free cysteine, were implicated as signalling metabolites controlling expression of a gene for a cysteine-rich seed storage protein.

Authors+Show Affiliations

CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, 2601 Australia. Linda.Tabe@csiro.auNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19939888

Citation

Tabe, Linda, et al. "Overexpression of Serine Acetlytransferase Produced Large Increases in O-acetylserine and Free Cysteine in Developing Seeds of a Grain Legume." Journal of Experimental Botany, vol. 61, no. 3, 2010, pp. 721-33.
Tabe L, Wirtz M, Molvig L, et al. Overexpression of serine acetlytransferase produced large increases in O-acetylserine and free cysteine in developing seeds of a grain legume. J Exp Bot. 2010;61(3):721-33.
Tabe, L., Wirtz, M., Molvig, L., Droux, M., & Hell, R. (2010). Overexpression of serine acetlytransferase produced large increases in O-acetylserine and free cysteine in developing seeds of a grain legume. Journal of Experimental Botany, 61(3), pp. 721-33. doi:10.1093/jxb/erp338.
Tabe L, et al. Overexpression of Serine Acetlytransferase Produced Large Increases in O-acetylserine and Free Cysteine in Developing Seeds of a Grain Legume. J Exp Bot. 2010;61(3):721-33. PubMed PMID: 19939888.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overexpression of serine acetlytransferase produced large increases in O-acetylserine and free cysteine in developing seeds of a grain legume. AU - Tabe,Linda, AU - Wirtz,Markus, AU - Molvig,Lisa, AU - Droux,Michel, AU - Hell,Ruediger, Y1 - 2009/11/25/ PY - 2009/11/27/entrez PY - 2009/11/27/pubmed PY - 2010/4/23/medline SP - 721 EP - 33 JF - Journal of experimental botany JO - J. Exp. Bot. VL - 61 IS - 3 N2 - There have been many attempts to increase concentrations of the nutritionally essential sulphur amino acids by modifying their biosynthetic pathway in leaves of transgenic plants. This report describes the first modification of cysteine biosynthesis in developing seeds; those of the grain legume, narrow leaf lupin (Lupinus angustifolius, L.). Expression in developing lupin embryos of a serine acetyltransferase (SAT) from Arabidopsis thaliana (AtSAT1 or AtSerat 2;1) was associated with increases of up to 5-fold in the concentrations of O-acetylserine (OAS), the immediate product of SAT, and up to 26-fold in free cysteine, resulting in some of the highest in vivo concentrations of these metabolites yet reported. Despite the dramatic changes in free cysteine in developing embryos of SAT overexpressers, concentrations of free methionine in developing embryos, and the total cysteine and methionine concentrations in mature seeds were not significantly altered. Pooled F(2) seeds segregating for the SAT transgene and for a transgene encoding a methionine- and cysteine-rich sunflower seed storage protein also had increased OAS and free cysteine, but not free methionine, during development, and no increase in mature seed total sulphur amino acids compared with controls lacking SAT overexpression. The data support the view that the cysteine biosynthetic pathway is active in developing seeds, and indicate that SAT activity limits cysteine biosynthesis, but that cysteine supply is not limiting for methionine biosynthesis or for storage protein synthesis in maturing lupin embryos in conditions of adequate sulphur nutrition. OAS and free methionine, but not free cysteine, were implicated as signalling metabolites controlling expression of a gene for a cysteine-rich seed storage protein. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/19939888/Overexpression_of_serine_acetlytransferase_produced_large_increases_in_O_acetylserine_and_free_cysteine_in_developing_seeds_of_a_grain_legume_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erp338 DB - PRIME DP - Unbound Medicine ER -