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Manipulation of alternative oxidase can influence salt tolerance in Arabidopsis thaliana.
Physiol Plant. 2009 Dec; 137(4):459-72.PP

Abstract

The growth and development of plants can be limited by environmental stresses such as salinity. It has been suggested that the non-phosphorylating alternative respiratory pathway in plants, mediated by the NAD(P)H dehydrogenase [NAD(P)H DH] and alternative oxidase (AOX), is important during environmental stresses. The involvement of this alternative pathway in a stress response may be linked to its capacity to uncouple carbon metabolism from adenylate control and/or the minimization of the formation of destructive reactive oxygen species (ROS). Salinity stress is a widespread, adverse environmental stress, which leads to an ionic imbalance, hyperosmotic stress and oxidative stress, the latter being the result of ROS formation. In this study, we show that salinity stress of Arabidopsis thaliana plants resulted in the formation of ROS, increased levels of Na+ in both the shoot and the root and an increase in transcription of Ataox1a, Atndb2 and Atndb4 genes, indicating the formation of an abridged non-phosphorylating electron transport chain in response to salinity stress. Furthermore, plants constitutively over-expressing Ataox1a, with increased AOX capacity, showed lower ROS formation, 30-40% improved growth rates and lower shoot Na+ content compared with controls, when grown under salinity stress conditions. Thus, more active AOX in roots and shoots can improve the salt tolerance of Arabidopsis as defined by its ability to grow more effectively in the presence of NaCl, and maintain lower shoot Na+ content. AOX does have an important role in stress adaptation in plants, and these results provide some validation of the hypothesis that AOX can play a critical role in cell re-programming under salinity stress.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Smith CA
School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide 5001, South Australia, Australia.
Melino VJ
No affiliation info available
Sweetman C
No affiliation info available
Soole KL
No affiliation info available

MeSH

ArabidopsisElectron TransportGene Expression Regulation, PlantMitochondrial ProteinsNADPH DehydrogenaseOxidative StressOxidoreductasesPhosphorylationPlant LeavesPlant ProteinsPlant RootsPlant ShootsPotassiumRNA, MessengerReactive Oxygen SpeciesSalt ToleranceSodiumSodium ChlorideStress, PhysiologicalTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19941623

Citation

Smith, Chevaun Anne, et al. "Manipulation of Alternative Oxidase Can Influence Salt Tolerance in Arabidopsis Thaliana." Physiologia Plantarum, vol. 137, no. 4, 2009, pp. 459-72.
Smith CA, Melino VJ, Sweetman C, et al. Manipulation of alternative oxidase can influence salt tolerance in Arabidopsis thaliana. Physiol Plant. 2009;137(4):459-72.
Smith, C. A., Melino, V. J., Sweetman, C., & Soole, K. L. (2009). Manipulation of alternative oxidase can influence salt tolerance in Arabidopsis thaliana. Physiologia Plantarum, 137(4), 459-72. https://doi.org/10.1111/j.1399-3054.2009.01305.x
Smith CA, et al. Manipulation of Alternative Oxidase Can Influence Salt Tolerance in Arabidopsis Thaliana. Physiol Plant. 2009;137(4):459-72. PubMed PMID: 19941623.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Manipulation of alternative oxidase can influence salt tolerance in Arabidopsis thaliana. AU - Smith,Chevaun Anne, AU - Melino,Vanessa Jane, AU - Sweetman,Crystal, AU - Soole,Kathleen Lydia, PY - 2009/11/28/entrez PY - 2009/11/28/pubmed PY - 2010/1/28/medline SP - 459 EP - 72 JF - Physiologia plantarum JO - Physiol Plant VL - 137 IS - 4 N2 - The growth and development of plants can be limited by environmental stresses such as salinity. It has been suggested that the non-phosphorylating alternative respiratory pathway in plants, mediated by the NAD(P)H dehydrogenase [NAD(P)H DH] and alternative oxidase (AOX), is important during environmental stresses. The involvement of this alternative pathway in a stress response may be linked to its capacity to uncouple carbon metabolism from adenylate control and/or the minimization of the formation of destructive reactive oxygen species (ROS). Salinity stress is a widespread, adverse environmental stress, which leads to an ionic imbalance, hyperosmotic stress and oxidative stress, the latter being the result of ROS formation. In this study, we show that salinity stress of Arabidopsis thaliana plants resulted in the formation of ROS, increased levels of Na+ in both the shoot and the root and an increase in transcription of Ataox1a, Atndb2 and Atndb4 genes, indicating the formation of an abridged non-phosphorylating electron transport chain in response to salinity stress. Furthermore, plants constitutively over-expressing Ataox1a, with increased AOX capacity, showed lower ROS formation, 30-40% improved growth rates and lower shoot Na+ content compared with controls, when grown under salinity stress conditions. Thus, more active AOX in roots and shoots can improve the salt tolerance of Arabidopsis as defined by its ability to grow more effectively in the presence of NaCl, and maintain lower shoot Na+ content. AOX does have an important role in stress adaptation in plants, and these results provide some validation of the hypothesis that AOX can play a critical role in cell re-programming under salinity stress. SN - 1399-3054 UR - https://www.unboundmedicine.com/medline/citation/19941623/Manipulation_of_alternative_oxidase_can_influence_salt_tolerance_in_Arabidopsis_thaliana_ L2 - https://doi.org/10.1111/j.1399-3054.2009.01305.x DB - PRIME DP - Unbound Medicine ER -
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