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Influence of peritoneal fluid on the expression of angiogenic and proteolytic factors in cultures of endometrial cells from women with endometriosis.
Hum Reprod. 2010 Feb; 25(2):398-405.HR

Abstract

BACKGROUND

Endometriosis, defined as the presence of endometrium outside the uterus, is one of the most frequent benign gynaecological diseases. It has been suggested that both endometrial and peritoneal factors, related to angiogenesis and proteolysis, can be implicated in this disease. The aim of this study was to evaluate the influence of peritoneal fluid on the expression of angiogenic and proteolytic factors in cultures of endometrial cells from women with and without endometriosis.

METHODS

Endometrial cells were isolated, cultured and treated with endometriotic or normal peritoneal fluid. Vascular endothelial growth factor-A (VEGF-A), urokinase plasminogen activator (uPA), matrix metalloproteinase-3 (MMP-3) and their inhibitors including thrombospondin-1, plasminogen activator inhibitor-1 and MMP inhibitor type 1 (TIMP-1) mRNA levels were evaluated by quantitative RT-PCR, and protein levels were quantified by ELISA.

RESULTS

Peritoneal fluid from women with endometriosis induced an increase in VEGF-A and uPA protein and VEGF-A mRNA and uPA mRNA levels in endometrial cell culture from women with (P < 0.01) and without endometriosis (P < 0.05). The highest levels of VEGF-A and uPA were observed in endometrial cell cultures from patients with endometriosis and treated with peritoneal fluid from women with endometriosis.

CONCLUSIONS

Peritoneal fluid from women with endometriosis induced more VEGF and uPA expression in endometrial cell culture from women with endometriosis than did normal peritoneal fluid. Endometrial-peritoneal interactions increased angiogenic and proteolytic factors in endometrial cells, which could contribute to the development of endometriotic lesions.

Authors+Show Affiliations

Research Center, Hospital Universitario La Fe, Valencia, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19945964

Citation

Cosín, R, et al. "Influence of Peritoneal Fluid On the Expression of Angiogenic and Proteolytic Factors in Cultures of Endometrial Cells From Women With Endometriosis." Human Reproduction (Oxford, England), vol. 25, no. 2, 2010, pp. 398-405.
Cosín R, Gilabert-Estellés J, Ramón LA, et al. Influence of peritoneal fluid on the expression of angiogenic and proteolytic factors in cultures of endometrial cells from women with endometriosis. Hum Reprod. 2010;25(2):398-405.
Cosín, R., Gilabert-Estellés, J., Ramón, L. A., Gómez-Lechón, M. J., Gilabert, J., Chirivella, M., Braza-Boïls, A., España, F., & Estellés, A. (2010). Influence of peritoneal fluid on the expression of angiogenic and proteolytic factors in cultures of endometrial cells from women with endometriosis. Human Reproduction (Oxford, England), 25(2), 398-405. https://doi.org/10.1093/humrep/dep419
Cosín R, et al. Influence of Peritoneal Fluid On the Expression of Angiogenic and Proteolytic Factors in Cultures of Endometrial Cells From Women With Endometriosis. Hum Reprod. 2010;25(2):398-405. PubMed PMID: 19945964.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of peritoneal fluid on the expression of angiogenic and proteolytic factors in cultures of endometrial cells from women with endometriosis. AU - Cosín,R, AU - Gilabert-Estellés,J, AU - Ramón,L A, AU - Gómez-Lechón,M J, AU - Gilabert,J, AU - Chirivella,M, AU - Braza-Boïls,A, AU - España,F, AU - Estellés,A, Y1 - 2009/11/26/ PY - 2009/12/1/entrez PY - 2009/12/1/pubmed PY - 2010/3/17/medline SP - 398 EP - 405 JF - Human reproduction (Oxford, England) JO - Hum. Reprod. VL - 25 IS - 2 N2 - BACKGROUND: Endometriosis, defined as the presence of endometrium outside the uterus, is one of the most frequent benign gynaecological diseases. It has been suggested that both endometrial and peritoneal factors, related to angiogenesis and proteolysis, can be implicated in this disease. The aim of this study was to evaluate the influence of peritoneal fluid on the expression of angiogenic and proteolytic factors in cultures of endometrial cells from women with and without endometriosis. METHODS: Endometrial cells were isolated, cultured and treated with endometriotic or normal peritoneal fluid. Vascular endothelial growth factor-A (VEGF-A), urokinase plasminogen activator (uPA), matrix metalloproteinase-3 (MMP-3) and their inhibitors including thrombospondin-1, plasminogen activator inhibitor-1 and MMP inhibitor type 1 (TIMP-1) mRNA levels were evaluated by quantitative RT-PCR, and protein levels were quantified by ELISA. RESULTS: Peritoneal fluid from women with endometriosis induced an increase in VEGF-A and uPA protein and VEGF-A mRNA and uPA mRNA levels in endometrial cell culture from women with (P < 0.01) and without endometriosis (P < 0.05). The highest levels of VEGF-A and uPA were observed in endometrial cell cultures from patients with endometriosis and treated with peritoneal fluid from women with endometriosis. CONCLUSIONS: Peritoneal fluid from women with endometriosis induced more VEGF and uPA expression in endometrial cell culture from women with endometriosis than did normal peritoneal fluid. Endometrial-peritoneal interactions increased angiogenic and proteolytic factors in endometrial cells, which could contribute to the development of endometriotic lesions. SN - 1460-2350 UR - https://www.unboundmedicine.com/medline/citation/19945964/Influence_of_peritoneal_fluid_on_the_expression_of_angiogenic_and_proteolytic_factors_in_cultures_of_endometrial_cells_from_women_with_endometriosis_ L2 - https://academic.oup.com/humrep/article-lookup/doi/10.1093/humrep/dep419 DB - PRIME DP - Unbound Medicine ER -