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Identification and phylogenetic analysis of an Orf virus isolated from an outbreak in sheep in the Jilin province of China.
Vet Microbiol 2010; 142(3-4):408-15VM

Abstract

This study investigated an acute outbreak of contagious ecthyma dermatitis that occurred in November 2008 in a herd of 180 small-tailed Han sheep in the Jilin province of China. The pathological findings of this case revealed severe vascular proliferation, viral cytopathic changes in keratinocytes by vacuolar degeneration, ballooning degeneration, and eosinophilic cytoplasmic inclusions, suggesting that the disease could have been caused by an Orf virus (ORFV) infection. Immunohistochemistry, indirect immunofluorescence (IFA), and transmission electron microscopy supported the diagnosis of ORFV infection. Finally, the pathogen of the disease was identified using polymerase chain reaction (PCR) and sequences of major envelope protein genes (ORFV 011(B2L) and ORFV 059(F1L)). The full-length ORFV 011(B2L) and ORFV 059(F1L) genes were cloned and sequenced. The nucleotide and amino acid sequences of ORFV 011(B2L) and ORFV 059(F1L) genes in these outbreaks were analyzed, and their phylogenetic trees were constructed. The phylogenetic studies of ORFV 011(B2L) genes and ORFV 059(F1L) genes showed that the ORFV-Jilin province isolate clustered in different branches and was closer to the ORFV-Mukteswar 67/04 isolate and the ORFV-OV/C2 isolate, respectively. To date, there is no report on the molecular characterization of any Orf virus with other isolates around the world in mainland China. Further, the above results may provide some insight into the genotype of the etiological agent responsible for the contagious ecthyma dermatitis outbreak in the Jilin province, and could also provide a comparative view of the central coding region genomics of parapoxvirus (PPV).

Authors+Show Affiliations

College of Animal Science and Veterinary Medicine, Jilin University, Changchun, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19948384

Citation

Zhao, Kui, et al. "Identification and Phylogenetic Analysis of an Orf Virus Isolated From an Outbreak in Sheep in the Jilin Province of China." Veterinary Microbiology, vol. 142, no. 3-4, 2010, pp. 408-15.
Zhao K, Song D, He W, et al. Identification and phylogenetic analysis of an Orf virus isolated from an outbreak in sheep in the Jilin province of China. Vet Microbiol. 2010;142(3-4):408-15.
Zhao, K., Song, D., He, W., Lu, H., Zhang, B., Li, C., ... Gao, F. (2010). Identification and phylogenetic analysis of an Orf virus isolated from an outbreak in sheep in the Jilin province of China. Veterinary Microbiology, 142(3-4), pp. 408-15. doi:10.1016/j.vetmic.2009.10.006.
Zhao K, et al. Identification and Phylogenetic Analysis of an Orf Virus Isolated From an Outbreak in Sheep in the Jilin Province of China. Vet Microbiol. 2010 May 19;142(3-4):408-15. PubMed PMID: 19948384.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and phylogenetic analysis of an Orf virus isolated from an outbreak in sheep in the Jilin province of China. AU - Zhao,Kui, AU - Song,Deguang, AU - He,Wenqi, AU - Lu,Huijun, AU - Zhang,Bingbing, AU - Li,Chao, AU - Chen,Keyan, AU - Gao,Feng, Y1 - 2009/10/31/ PY - 2009/06/18/received PY - 2009/09/26/revised PY - 2009/10/02/accepted PY - 2009/12/2/entrez PY - 2009/12/2/pubmed PY - 2010/8/10/medline SP - 408 EP - 15 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 142 IS - 3-4 N2 - This study investigated an acute outbreak of contagious ecthyma dermatitis that occurred in November 2008 in a herd of 180 small-tailed Han sheep in the Jilin province of China. The pathological findings of this case revealed severe vascular proliferation, viral cytopathic changes in keratinocytes by vacuolar degeneration, ballooning degeneration, and eosinophilic cytoplasmic inclusions, suggesting that the disease could have been caused by an Orf virus (ORFV) infection. Immunohistochemistry, indirect immunofluorescence (IFA), and transmission electron microscopy supported the diagnosis of ORFV infection. Finally, the pathogen of the disease was identified using polymerase chain reaction (PCR) and sequences of major envelope protein genes (ORFV 011(B2L) and ORFV 059(F1L)). The full-length ORFV 011(B2L) and ORFV 059(F1L) genes were cloned and sequenced. The nucleotide and amino acid sequences of ORFV 011(B2L) and ORFV 059(F1L) genes in these outbreaks were analyzed, and their phylogenetic trees were constructed. The phylogenetic studies of ORFV 011(B2L) genes and ORFV 059(F1L) genes showed that the ORFV-Jilin province isolate clustered in different branches and was closer to the ORFV-Mukteswar 67/04 isolate and the ORFV-OV/C2 isolate, respectively. To date, there is no report on the molecular characterization of any Orf virus with other isolates around the world in mainland China. Further, the above results may provide some insight into the genotype of the etiological agent responsible for the contagious ecthyma dermatitis outbreak in the Jilin province, and could also provide a comparative view of the central coding region genomics of parapoxvirus (PPV). SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/19948384/Identification_and_phylogenetic_analysis_of_an_Orf_virus_isolated_from_an_outbreak_in_sheep_in_the_Jilin_province_of_China_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(09)00520-3 DB - PRIME DP - Unbound Medicine ER -