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Long-term shedding of hepatitis E virus in the feces of pigs infected naturally, born to sows with and without maternal antibodies.
J Med Virol. 2010 Jan; 82(1):69-76.JM

Abstract

Pigs are presumed reservoirs for hepatitis E virus (HEV) transmission to humans. To examine infection kinetics, two litters of domestic pigs (A and B, each containing 10 piglets) infected naturally with HEV were studied until pigs were 6 months old. Maternal IgG and IgA antibodies were detected in litter A piglets, but not in litter B ones. All pigs shed HEV in feces when they were 30-110 days old, and 17 developed viremia at 40-100 days of age. Phylogenetic analysis revealed a highly close sequence of HEV genotype 3 in all pigs. The serum levels of specific IgG and IgA were similar in all pigs, although IgA was not detected in the feces. Interestingly, the onset of both viremia and seroconversion was delayed significantly in litter A pigs. The kinetics of fecal virus shedding was similar in both litters; shedding was not detected after the pigs were 120 days old. The differences in the infection kinetics between litters A and B suggested that maternal antibodies delayed the onset of viremia and seroconversion. Quantitative real-time reverse transcriptase-polymerase chain reaction revealed that HEV RNA in feces peaked 10 days after initial shedding of approximately 10(6.0) copies/g. The viral load was much lower in the serum than in the feces. At 200 days of age, HEV RNA was found in the internal organs of 3 out of 13 pigs. These study findings improve the understanding of the dynamics of natural HEV transmission in pigs, which could help in controlling virus transmission from pigs to humans.

Authors+Show Affiliations

School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan. k-hagi@rakuno.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19950246

Citation

Kanai, Yuta, et al. "Long-term Shedding of Hepatitis E Virus in the Feces of Pigs Infected Naturally, Born to Sows With and Without Maternal Antibodies." Journal of Medical Virology, vol. 82, no. 1, 2010, pp. 69-76.
Kanai Y, Tsujikawa M, Yunoki M, et al. Long-term shedding of hepatitis E virus in the feces of pigs infected naturally, born to sows with and without maternal antibodies. J Med Virol. 2010;82(1):69-76.
Kanai, Y., Tsujikawa, M., Yunoki, M., Nishiyama, S., Ikuta, K., & Hagiwara, K. (2010). Long-term shedding of hepatitis E virus in the feces of pigs infected naturally, born to sows with and without maternal antibodies. Journal of Medical Virology, 82(1), 69-76. https://doi.org/10.1002/jmv.21647
Kanai Y, et al. Long-term Shedding of Hepatitis E Virus in the Feces of Pigs Infected Naturally, Born to Sows With and Without Maternal Antibodies. J Med Virol. 2010;82(1):69-76. PubMed PMID: 19950246.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long-term shedding of hepatitis E virus in the feces of pigs infected naturally, born to sows with and without maternal antibodies. AU - Kanai,Yuta, AU - Tsujikawa,Muneo, AU - Yunoki,Mikihiro, AU - Nishiyama,Shoko, AU - Ikuta,Kazuyoshi, AU - Hagiwara,Katsuro, PY - 2009/12/2/entrez PY - 2009/12/2/pubmed PY - 2010/2/27/medline SP - 69 EP - 76 JF - Journal of medical virology JO - J Med Virol VL - 82 IS - 1 N2 - Pigs are presumed reservoirs for hepatitis E virus (HEV) transmission to humans. To examine infection kinetics, two litters of domestic pigs (A and B, each containing 10 piglets) infected naturally with HEV were studied until pigs were 6 months old. Maternal IgG and IgA antibodies were detected in litter A piglets, but not in litter B ones. All pigs shed HEV in feces when they were 30-110 days old, and 17 developed viremia at 40-100 days of age. Phylogenetic analysis revealed a highly close sequence of HEV genotype 3 in all pigs. The serum levels of specific IgG and IgA were similar in all pigs, although IgA was not detected in the feces. Interestingly, the onset of both viremia and seroconversion was delayed significantly in litter A pigs. The kinetics of fecal virus shedding was similar in both litters; shedding was not detected after the pigs were 120 days old. The differences in the infection kinetics between litters A and B suggested that maternal antibodies delayed the onset of viremia and seroconversion. Quantitative real-time reverse transcriptase-polymerase chain reaction revealed that HEV RNA in feces peaked 10 days after initial shedding of approximately 10(6.0) copies/g. The viral load was much lower in the serum than in the feces. At 200 days of age, HEV RNA was found in the internal organs of 3 out of 13 pigs. These study findings improve the understanding of the dynamics of natural HEV transmission in pigs, which could help in controlling virus transmission from pigs to humans. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/19950246/Long_term_shedding_of_hepatitis_E_virus_in_the_feces_of_pigs_infected_naturally_born_to_sows_with_and_without_maternal_antibodies_ L2 - https://doi.org/10.1002/jmv.21647 DB - PRIME DP - Unbound Medicine ER -