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Partial analysis of hepatitis B virus DNA from hepatocellular carcinoma showing negative hepatitis B virus surface antigen: an analysis of two Japanese cases.
Hepatogastroenterology. 2009 Sep-Oct; 56(94-95):1516-20.H

Abstract

It has been reported that hepatitis B virus (HBV) DNA is detected in serum and/or liver in patients with hepatocellular carcinoma (HCC) without HBsAg. To adress this issue, we analyzed HBV genome in 2 HCC cases without HBsAg. The DNA from serum from patients with HCC was amplified with a nested PCR, and 'a' determinant of S region, core promoter region and precore region were sequenced. The first case, a 50 years-old male, was negative for HBsAg and HBeAg, and positive for anti-HBs, anti-HBe and anti-HBc. Viral load of HBV in serum was 4.0 log genome equivalent/ml by TMA assay, and was 1.1 X 105 copy/ml by real-time PCR system. A nucleotide analysis of the common 'a' determinant of S gene showed that the 5 first amino acids of 'a' determinant, CTIPA, were changed to CKTCTTPA. The second case, a 76 years-old male, was positive for anti-HBe, but negative for HBsAg, anti-HBs, HBeAg and anti-HBc. No missense or nonsense mutations were seen in 'a' determinant of S region. Viral load of serum HBV was < 3.7 log genome equivalent/ml by TMA assay, but was 2.4X103 copy/ml by real-time PCR system. The results of the present study suggest that the mechanisms of HBsAg loss are diverse among HCC patients without HBsAg, and that an analysis of HBV genome is a useful tool to dissolve molecular mechanisms losing HBs antigenicity.

Authors+Show Affiliations

Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, Japan. gshiota@med.tottori-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

19950820

Citation

Shiota, Goshi, et al. "Partial Analysis of Hepatitis B Virus DNA From Hepatocellular Carcinoma Showing Negative Hepatitis B Virus Surface Antigen: an Analysis of Two Japanese Cases." Hepato-gastroenterology, vol. 56, no. 94-95, 2009, pp. 1516-20.
Shiota G, Oyama K, Udagawa A, et al. Partial analysis of hepatitis B virus DNA from hepatocellular carcinoma showing negative hepatitis B virus surface antigen: an analysis of two Japanese cases. Hepatogastroenterology. 2009;56(94-95):1516-20.
Shiota, G., Oyama, K., Udagawa, A., Nomi, T., Tanaka, K., Tsutsumi, A., Noguchi, N., Okano, J., Kishimoto, Y., Kanbe, T., Kishimoto, H., & Kawasaki, H. (2009). Partial analysis of hepatitis B virus DNA from hepatocellular carcinoma showing negative hepatitis B virus surface antigen: an analysis of two Japanese cases. Hepato-gastroenterology, 56(94-95), 1516-20.
Shiota G, et al. Partial Analysis of Hepatitis B Virus DNA From Hepatocellular Carcinoma Showing Negative Hepatitis B Virus Surface Antigen: an Analysis of Two Japanese Cases. Hepatogastroenterology. 2009 Sep-Oct;56(94-95):1516-20. PubMed PMID: 19950820.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Partial analysis of hepatitis B virus DNA from hepatocellular carcinoma showing negative hepatitis B virus surface antigen: an analysis of two Japanese cases. AU - Shiota,Goshi, AU - Oyama,Kenji, AU - Udagawa,Akihide, AU - Nomi,Takahiro, AU - Tanaka,Kiwamu, AU - Tsutsumi,Atsushi, AU - Noguchi,Noya, AU - Okano,Jun-ichi, AU - Kishimoto,Yosuke, AU - Kanbe,Takamasa, AU - Kishimoto,Hiroyuki, AU - Kawasaki,Hironaka, PY - 2009/12/3/entrez PY - 2009/12/3/pubmed PY - 2009/12/16/medline SP - 1516 EP - 20 JF - Hepato-gastroenterology JO - Hepatogastroenterology VL - 56 IS - 94-95 N2 - It has been reported that hepatitis B virus (HBV) DNA is detected in serum and/or liver in patients with hepatocellular carcinoma (HCC) without HBsAg. To adress this issue, we analyzed HBV genome in 2 HCC cases without HBsAg. The DNA from serum from patients with HCC was amplified with a nested PCR, and 'a' determinant of S region, core promoter region and precore region were sequenced. The first case, a 50 years-old male, was negative for HBsAg and HBeAg, and positive for anti-HBs, anti-HBe and anti-HBc. Viral load of HBV in serum was 4.0 log genome equivalent/ml by TMA assay, and was 1.1 X 105 copy/ml by real-time PCR system. A nucleotide analysis of the common 'a' determinant of S gene showed that the 5 first amino acids of 'a' determinant, CTIPA, were changed to CKTCTTPA. The second case, a 76 years-old male, was positive for anti-HBe, but negative for HBsAg, anti-HBs, HBeAg and anti-HBc. No missense or nonsense mutations were seen in 'a' determinant of S region. Viral load of serum HBV was < 3.7 log genome equivalent/ml by TMA assay, but was 2.4X103 copy/ml by real-time PCR system. The results of the present study suggest that the mechanisms of HBsAg loss are diverse among HCC patients without HBsAg, and that an analysis of HBV genome is a useful tool to dissolve molecular mechanisms losing HBs antigenicity. SN - 0172-6390 UR - https://www.unboundmedicine.com/medline/citation/19950820/Partial_analysis_of_hepatitis_B_virus_DNA_from_hepatocellular_carcinoma_showing_negative_hepatitis_B_virus_surface_antigen:_an_analysis_of_two_Japanese_cases_ L2 - http://www.diseaseinfosearch.org/result/3332 DB - PRIME DP - Unbound Medicine ER -