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[Relationship between copies of cytomegalovirus in plasma and cytomegalovirus disease after allogeneic hematopoietic stem cell transplantation].
Zhonghua Yi Xue Za Zhi. 2009 Jun 09; 89(22):1540-3.ZY

Abstract

OBJECTIVE

To evaluate the clinical significance of using real-time quantitative polymerase chain reaction (RQ-PCR) in monitoring cytomegalovirus infection in allogenic hemopoietic stem-cell transplant (allo-HSCT) recipients.

METHODS

A total of 318 patients who received allo-HSCT in the past 2 years were analyzed retrospectively. RQ-PCR was performed to monitor CMV viremia twice a week after transplantation.

RESULTS

CMV-DNA was detected in the plasma of 136 patients. The median time for the occurrence of CMV-DNA was 42 days after HSCT. The highest CMV-DNA load was 1.5 x 10(4) copies/ml while the CMV-DNA load at onset of reactivation was 4.5 x 10(3) copies/ml. CMV pneumonia or CMV enteritis occurred in 23 patients and the incidence of these CMV diseases is 7.2%. The CMV-DNA was detectable before CMV disease among 14 patients and after the clinical manifestation of CMV disease in 4 patients, while 5 patients were diagnosed with CMV disease having no plasma CMV-DNA positive at all. The highest CMV-DNA load was higher in the CMV patients than those with no CMV disease (4.3 x 10(4) copies/ml and 1.3 x 10(4) copies/ml, P = 0.009) although the initial load had no difference (3.7 x 10(3) copies/m vs 4.7 x 10(3) copies/ml, P = 0.63). The highest CMV-DNA load also rose with the occurring frequency of CMV infection occurred from 1 to 4 times. The median values of CMV-DNA were 82.6 x 10(2), 261.3 x 10(2), 440.8 x 10(2) and 10,659.0 x 10(2) copies/ml (P < 0.01) respectively. Meanwhile, the incidence of CMV disease also increased markedly from 2.7% (5/182) to 50% (2/4) (P = 0.001).

CONCLUSION

Detection of CMV-DNA in plasma by RQ-PCR appears to be an effective prognostic predictor of CMV diseases. High CMV-DNA load and repeated viremia indicate a high incidence of CMV diseases.

Authors+Show Affiliations

Hematology Institute, Peking University People's Hospital, Beijing 100044, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

19953881

Citation

Chen, Yu-Hong, et al. "[Relationship Between Copies of Cytomegalovirus in Plasma and Cytomegalovirus Disease After Allogeneic Hematopoietic Stem Cell Transplantation]." Zhonghua Yi Xue Za Zhi, vol. 89, no. 22, 2009, pp. 1540-3.
Chen YH, Zhao XS, Liu KY, et al. [Relationship between copies of cytomegalovirus in plasma and cytomegalovirus disease after allogeneic hematopoietic stem cell transplantation]. Zhonghua Yi Xue Za Zhi. 2009;89(22):1540-3.
Chen, Y. H., Zhao, X. S., Liu, K. Y., Xu, L. P., Liu, D. H., Chen, H., Zhang, X. H., Han, W., Wang, Y., Zhao, T., Zhao, X. T., & Huang, X. J. (2009). [Relationship between copies of cytomegalovirus in plasma and cytomegalovirus disease after allogeneic hematopoietic stem cell transplantation]. Zhonghua Yi Xue Za Zhi, 89(22), 1540-3.
Chen YH, et al. [Relationship Between Copies of Cytomegalovirus in Plasma and Cytomegalovirus Disease After Allogeneic Hematopoietic Stem Cell Transplantation]. Zhonghua Yi Xue Za Zhi. 2009 Jun 9;89(22):1540-3. PubMed PMID: 19953881.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Relationship between copies of cytomegalovirus in plasma and cytomegalovirus disease after allogeneic hematopoietic stem cell transplantation]. AU - Chen,Yu-Hong, AU - Zhao,Xiao-Su, AU - Liu,Kai-Yan, AU - Xu,Lan-Ping, AU - Liu,Dai-Hong, AU - Chen,Huan, AU - Zhang,Xiao-Hui, AU - Han,Wei, AU - Wang,Yu, AU - Zhao,Ting, AU - Zhao,Xiao-Tao, AU - Huang,Xiao-Jun, PY - 2009/12/4/entrez PY - 2009/12/4/pubmed PY - 2010/2/17/medline SP - 1540 EP - 3 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 89 IS - 22 N2 - OBJECTIVE: To evaluate the clinical significance of using real-time quantitative polymerase chain reaction (RQ-PCR) in monitoring cytomegalovirus infection in allogenic hemopoietic stem-cell transplant (allo-HSCT) recipients. METHODS: A total of 318 patients who received allo-HSCT in the past 2 years were analyzed retrospectively. RQ-PCR was performed to monitor CMV viremia twice a week after transplantation. RESULTS: CMV-DNA was detected in the plasma of 136 patients. The median time for the occurrence of CMV-DNA was 42 days after HSCT. The highest CMV-DNA load was 1.5 x 10(4) copies/ml while the CMV-DNA load at onset of reactivation was 4.5 x 10(3) copies/ml. CMV pneumonia or CMV enteritis occurred in 23 patients and the incidence of these CMV diseases is 7.2%. The CMV-DNA was detectable before CMV disease among 14 patients and after the clinical manifestation of CMV disease in 4 patients, while 5 patients were diagnosed with CMV disease having no plasma CMV-DNA positive at all. The highest CMV-DNA load was higher in the CMV patients than those with no CMV disease (4.3 x 10(4) copies/ml and 1.3 x 10(4) copies/ml, P = 0.009) although the initial load had no difference (3.7 x 10(3) copies/m vs 4.7 x 10(3) copies/ml, P = 0.63). The highest CMV-DNA load also rose with the occurring frequency of CMV infection occurred from 1 to 4 times. The median values of CMV-DNA were 82.6 x 10(2), 261.3 x 10(2), 440.8 x 10(2) and 10,659.0 x 10(2) copies/ml (P < 0.01) respectively. Meanwhile, the incidence of CMV disease also increased markedly from 2.7% (5/182) to 50% (2/4) (P = 0.001). CONCLUSION: Detection of CMV-DNA in plasma by RQ-PCR appears to be an effective prognostic predictor of CMV diseases. High CMV-DNA load and repeated viremia indicate a high incidence of CMV diseases. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/19953881/[Relationship_between_copies_of_cytomegalovirus_in_plasma_and_cytomegalovirus_disease_after_allogeneic_hematopoietic_stem_cell_transplantation]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0376-2491&amp;year=2009&amp;vol=89&amp;issue=22&amp;fpage=1540 DB - PRIME DP - Unbound Medicine ER -