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Dietary fish oil n-3 polyunsaturated fatty acids and alpha-linolenic acid differently affect brain accretion of docosahexaenoic acid and expression of desaturases and sterol regulatory element-binding protein 1 in mice.

Abstract

Whether preformed dietary docosahexaenoic acid (DHA) is required for brain accretion has not been clearly determined. In this study, we investigated in mice the different effects of dietary longer-chain n-3 polyunsaturated fatty acids (PUFAs) and α-linolenic acid (LNA) on brain accretion of DHA and the expression of associated desaturases and transcription factors. C57 BL/6J mice were fed for 3 months with four fish oil n-3 PUFA diets--lower, low, high and higher (0.46%, 0.91%, 1.73% and 4.29% total energy, respectively); a flaxseed oil n-3 PUFA (5.01% total energy) diet; and an n-3 PUFA-deficient diet, respectively. Either fish oil or flaxseed oil n-3 PUFA diets increased DHA concentrations in the brain. However, the flaxseed oil n-3 PUFA diet was less effective than the fish oil diet with higher amount of n-3 PUFA in increasing brain DHA content. Furthermore, the expressions of delta-6 desaturase (D6D) and sterol regulatory element binding protein 1 (SREBP-1) in the liver were down-regulated by all fish oil diets with different amounts of n-3 PUFAs, as well as by the flaxseed oil n-3 PUFA diet, whereas in the brain, D6D, delta-5 desaturase (D5D) and SREBP-1 expressions were down-regulated by the higher fish oil n-3 PUFA diet rather than by other fish oil n-3 PUFA and the flaxseed oil n-3 PUFA diets. These results suggest that preformed dietary DHA, different from those converted by LNA inside the body, is better for brain accretion. Dietary longer-chain n-3 PUFAs affect expressions of D6D, D5D and SREBP-1 in the brain differently from their precursor LNA.

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  • Authors+Show Affiliations

    ,

    Clinical Nutrition Center, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.

    , , , ,

    Source

    MeSH

    Animals
    Base Sequence
    Brain
    DNA Primers
    Dietary Fats, Unsaturated
    Docosahexaenoic Acids
    Fatty Acid Desaturases
    Fatty Acids, Omega-3
    Fish Oils
    Liver
    Mice
    Mice, Inbred C57BL
    Reverse Transcriptase Polymerase Chain Reaction
    Sterol Regulatory Element Binding Protein 1
    alpha-Linolenic Acid

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    19954955

    Citation

    Zhu, Haiyan, et al. "Dietary Fish Oil N-3 Polyunsaturated Fatty Acids and Alpha-linolenic Acid Differently Affect Brain Accretion of Docosahexaenoic Acid and Expression of Desaturases and Sterol Regulatory Element-binding Protein 1 in Mice." The Journal of Nutritional Biochemistry, vol. 21, no. 10, 2010, pp. 954-60.
    Zhu H, Fan C, Xu F, et al. Dietary fish oil n-3 polyunsaturated fatty acids and alpha-linolenic acid differently affect brain accretion of docosahexaenoic acid and expression of desaturases and sterol regulatory element-binding protein 1 in mice. J Nutr Biochem. 2010;21(10):954-60.
    Zhu, H., Fan, C., Xu, F., Tian, C., Zhang, F., & Qi, K. (2010). Dietary fish oil n-3 polyunsaturated fatty acids and alpha-linolenic acid differently affect brain accretion of docosahexaenoic acid and expression of desaturases and sterol regulatory element-binding protein 1 in mice. The Journal of Nutritional Biochemistry, 21(10), pp. 954-60. doi:10.1016/j.jnutbio.2009.07.011.
    Zhu H, et al. Dietary Fish Oil N-3 Polyunsaturated Fatty Acids and Alpha-linolenic Acid Differently Affect Brain Accretion of Docosahexaenoic Acid and Expression of Desaturases and Sterol Regulatory Element-binding Protein 1 in Mice. J Nutr Biochem. 2010;21(10):954-60. PubMed PMID: 19954955.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Dietary fish oil n-3 polyunsaturated fatty acids and alpha-linolenic acid differently affect brain accretion of docosahexaenoic acid and expression of desaturases and sterol regulatory element-binding protein 1 in mice. AU - Zhu,Haiyan, AU - Fan,Chaonan, AU - Xu,Feng, AU - Tian,Chunyu, AU - Zhang,Feng, AU - Qi,Kemin, Y1 - 2009/12/01/ PY - 2008/09/12/received PY - 2009/04/16/revised PY - 2009/07/14/accepted PY - 2009/12/4/entrez PY - 2009/12/4/pubmed PY - 2011/2/1/medline SP - 954 EP - 60 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 21 IS - 10 N2 - Whether preformed dietary docosahexaenoic acid (DHA) is required for brain accretion has not been clearly determined. In this study, we investigated in mice the different effects of dietary longer-chain n-3 polyunsaturated fatty acids (PUFAs) and α-linolenic acid (LNA) on brain accretion of DHA and the expression of associated desaturases and transcription factors. C57 BL/6J mice were fed for 3 months with four fish oil n-3 PUFA diets--lower, low, high and higher (0.46%, 0.91%, 1.73% and 4.29% total energy, respectively); a flaxseed oil n-3 PUFA (5.01% total energy) diet; and an n-3 PUFA-deficient diet, respectively. Either fish oil or flaxseed oil n-3 PUFA diets increased DHA concentrations in the brain. However, the flaxseed oil n-3 PUFA diet was less effective than the fish oil diet with higher amount of n-3 PUFA in increasing brain DHA content. Furthermore, the expressions of delta-6 desaturase (D6D) and sterol regulatory element binding protein 1 (SREBP-1) in the liver were down-regulated by all fish oil diets with different amounts of n-3 PUFAs, as well as by the flaxseed oil n-3 PUFA diet, whereas in the brain, D6D, delta-5 desaturase (D5D) and SREBP-1 expressions were down-regulated by the higher fish oil n-3 PUFA diet rather than by other fish oil n-3 PUFA and the flaxseed oil n-3 PUFA diets. These results suggest that preformed dietary DHA, different from those converted by LNA inside the body, is better for brain accretion. Dietary longer-chain n-3 PUFAs affect expressions of D6D, D5D and SREBP-1 in the brain differently from their precursor LNA. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/19954955/Dietary_fish_oil_n_3_polyunsaturated_fatty_acids_and_alpha_linolenic_acid_differently_affect_brain_accretion_of_docosahexaenoic_acid_and_expression_of_desaturases_and_sterol_regulatory_element_binding_protein_1_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(09)00171-5 DB - PRIME DP - Unbound Medicine ER -