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Functional modulation of satellite cells in long-term denervated human laryngeal muscle.
Laryngoscope. 2010 Feb; 120(2):353-8.L

Abstract

OBJECTIVES/HYPOTHESIS

To evaluate the effects of long-term denervation on satellite cells (SCs) as myogenic stem cells in human posterior cricoarytenoid (PCA) muscle.

STUDY DESIGN

Histological investigation of SCs and quantitative assessment of myoD and myogenin, which are two key myogenic regulatory factors.

METHODS

According to the course of denervation, denervated PCA muscles of 58 patients who suffered from traumatic unilateral vocal cord paralysis were divided into four groups: group A (6-12 months, 15 cases), group B (13-24 months, 17 cases), group C (25-36 months, 14 cases), and group D (more than 36 months, 12 cases). Normal PCA muscles (12 cases) were used as a control group. Immunofluorescence labeling was used to visualize the SCs. Transcription and protein expression levels of myoD and myogenin were assessed using real-time quantitative polymerase chain reaction and Western blots, respectively.

RESULTS

MyoD- and myogenin-positive cells and embryonic myosin heavy chain-positive myofibers were detected in group A and most of the samples of group B. Transcription levels of myoD and myogenin were highly upregulated in groups A and B, whereas groups C and D showed no significant difference as compared to control. Protein expression levels of myoD and myogenin peaked in group A, which was significantly different than group B. In contrast, no expression was observed in the other groups.

CONCLUSIONS

Activated SCs contribute to regenerative myogenesis in denervated laryngeal muscles through compensatory mechanisms. Laryngeal muscles exhibit persistent regenerative potential from the viewpoint of muscle SCs after less than 2 years of denervation.

Authors+Show Affiliations

Department of Otorhinolaryngology-Head and Neck Surgery, Changhai Hospital, Second Military Medical University, Shanghai, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19957344

Citation

Donghui, Chen, et al. "Functional Modulation of Satellite Cells in Long-term Denervated Human Laryngeal Muscle." The Laryngoscope, vol. 120, no. 2, 2010, pp. 353-8.
Donghui C, Shicai C, Wei W, et al. Functional modulation of satellite cells in long-term denervated human laryngeal muscle. Laryngoscope. 2010;120(2):353-8.
Donghui, C., Shicai, C., Wei, W., Fei, L., Jianjun, J., Gang, C., & Hongliang, Z. (2010). Functional modulation of satellite cells in long-term denervated human laryngeal muscle. The Laryngoscope, 120(2), 353-8. https://doi.org/10.1002/lary.20796
Donghui C, et al. Functional Modulation of Satellite Cells in Long-term Denervated Human Laryngeal Muscle. Laryngoscope. 2010;120(2):353-8. PubMed PMID: 19957344.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional modulation of satellite cells in long-term denervated human laryngeal muscle. AU - Donghui,Chen, AU - Shicai,Chen, AU - Wei,Wang, AU - Fei,Liu, AU - Jianjun,Jing, AU - Gang,Chen, AU - Hongliang,Zheng, PY - 2009/12/4/entrez PY - 2009/12/4/pubmed PY - 2010/2/17/medline SP - 353 EP - 8 JF - The Laryngoscope JO - Laryngoscope VL - 120 IS - 2 N2 - OBJECTIVES/HYPOTHESIS: To evaluate the effects of long-term denervation on satellite cells (SCs) as myogenic stem cells in human posterior cricoarytenoid (PCA) muscle. STUDY DESIGN: Histological investigation of SCs and quantitative assessment of myoD and myogenin, which are two key myogenic regulatory factors. METHODS: According to the course of denervation, denervated PCA muscles of 58 patients who suffered from traumatic unilateral vocal cord paralysis were divided into four groups: group A (6-12 months, 15 cases), group B (13-24 months, 17 cases), group C (25-36 months, 14 cases), and group D (more than 36 months, 12 cases). Normal PCA muscles (12 cases) were used as a control group. Immunofluorescence labeling was used to visualize the SCs. Transcription and protein expression levels of myoD and myogenin were assessed using real-time quantitative polymerase chain reaction and Western blots, respectively. RESULTS: MyoD- and myogenin-positive cells and embryonic myosin heavy chain-positive myofibers were detected in group A and most of the samples of group B. Transcription levels of myoD and myogenin were highly upregulated in groups A and B, whereas groups C and D showed no significant difference as compared to control. Protein expression levels of myoD and myogenin peaked in group A, which was significantly different than group B. In contrast, no expression was observed in the other groups. CONCLUSIONS: Activated SCs contribute to regenerative myogenesis in denervated laryngeal muscles through compensatory mechanisms. Laryngeal muscles exhibit persistent regenerative potential from the viewpoint of muscle SCs after less than 2 years of denervation. SN - 1531-4995 UR - https://www.unboundmedicine.com/medline/citation/19957344/Functional_modulation_of_satellite_cells_in_long_term_denervated_human_laryngeal_muscle_ L2 - https://doi.org/10.1002/lary.20796 DB - PRIME DP - Unbound Medicine ER -