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Enhancement of interleukin-1beta-induced iNOS expression in cultured vascular smooth muscle cells of Goto-Kakizaki diabetes rats.
Eur J Pharmacol 2010; 629(1-3):1-6EJ

Abstract

The purpose of this study was to determine whether expression of inducible nitric oxide synthase (iNOS) is altered in vascular smooth muscle cells of type 2 diabetes rats. We used cultured aortic smooth muscle cells (ASMCs) isolated from male Goto-Kakizaki diabetes rats (G-K rats) aged 27-28weeks and age-matched Wistar rats (control rats). iNOS and extracellular signal-regulated kinase (ERK) were evaluated by immunoblot and/or immunochemical analyses, and NO production was evaluated by measuring NO(X) (NO(2) and NO(3)). Expression of iNOS was not detected in ASMCs of either G-K or control rats under a resting condition. Stimulation with interleukin-1beta (IL-1beta) induced iNOS expression, which was much greater in ASMCs from G-K rats than in ASMCs from control rats. When ASMCs were stimulated with IL-1beta, the number of iNOS-immunoreactive ASMCs from G-K rats increased more prominently than did the number of such ASMCs from control rats. IL-1beta-induced NO production was also much greater in ASMCs from G-K rats than in those from control rats. Both IL-1beta-induced iNOS expression and NO production in ASMCs of G-K and control rats were markedly reduced in the presence of an ERK inhibitor, U0126 or PD98059. Both basal and IL-1beta-stimulated levels of ERK activity were significantly higher in ASMCs from G-K rats than in ASMCs from control rats. The results suggest that iNOS induction is enhanced in cultured ASMCs from G-K rats and that this enhancement is associated with increased ERK activity.

Authors+Show Affiliations

Department of Environmental and Preventive Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan. wakabaya@hyo-med.ac.jpNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19958762

Citation

Wakabayashi, Ichiro, et al. "Enhancement of Interleukin-1beta-induced iNOS Expression in Cultured Vascular Smooth Muscle Cells of Goto-Kakizaki Diabetes Rats." European Journal of Pharmacology, vol. 629, no. 1-3, 2010, pp. 1-6.
Wakabayashi I, Nakano T, Takahashi Y. Enhancement of interleukin-1beta-induced iNOS expression in cultured vascular smooth muscle cells of Goto-Kakizaki diabetes rats. Eur J Pharmacol. 2010;629(1-3):1-6.
Wakabayashi, I., Nakano, T., & Takahashi, Y. (2010). Enhancement of interleukin-1beta-induced iNOS expression in cultured vascular smooth muscle cells of Goto-Kakizaki diabetes rats. European Journal of Pharmacology, 629(1-3), pp. 1-6. doi:10.1016/j.ejphar.2009.11.054.
Wakabayashi I, Nakano T, Takahashi Y. Enhancement of Interleukin-1beta-induced iNOS Expression in Cultured Vascular Smooth Muscle Cells of Goto-Kakizaki Diabetes Rats. Eur J Pharmacol. 2010 Mar 10;629(1-3):1-6. PubMed PMID: 19958762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhancement of interleukin-1beta-induced iNOS expression in cultured vascular smooth muscle cells of Goto-Kakizaki diabetes rats. AU - Wakabayashi,Ichiro, AU - Nakano,Tomoyuki, AU - Takahashi,Yuji, Y1 - 2009/12/01/ PY - 2009/07/23/received PY - 2009/11/18/revised PY - 2009/11/24/accepted PY - 2009/12/5/entrez PY - 2009/12/5/pubmed PY - 2010/3/31/medline SP - 1 EP - 6 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 629 IS - 1-3 N2 - The purpose of this study was to determine whether expression of inducible nitric oxide synthase (iNOS) is altered in vascular smooth muscle cells of type 2 diabetes rats. We used cultured aortic smooth muscle cells (ASMCs) isolated from male Goto-Kakizaki diabetes rats (G-K rats) aged 27-28weeks and age-matched Wistar rats (control rats). iNOS and extracellular signal-regulated kinase (ERK) were evaluated by immunoblot and/or immunochemical analyses, and NO production was evaluated by measuring NO(X) (NO(2) and NO(3)). Expression of iNOS was not detected in ASMCs of either G-K or control rats under a resting condition. Stimulation with interleukin-1beta (IL-1beta) induced iNOS expression, which was much greater in ASMCs from G-K rats than in ASMCs from control rats. When ASMCs were stimulated with IL-1beta, the number of iNOS-immunoreactive ASMCs from G-K rats increased more prominently than did the number of such ASMCs from control rats. IL-1beta-induced NO production was also much greater in ASMCs from G-K rats than in those from control rats. Both IL-1beta-induced iNOS expression and NO production in ASMCs of G-K and control rats were markedly reduced in the presence of an ERK inhibitor, U0126 or PD98059. Both basal and IL-1beta-stimulated levels of ERK activity were significantly higher in ASMCs from G-K rats than in ASMCs from control rats. The results suggest that iNOS induction is enhanced in cultured ASMCs from G-K rats and that this enhancement is associated with increased ERK activity. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/19958762/Enhancement_of_interleukin_1beta_induced_iNOS_expression_in_cultured_vascular_smooth_muscle_cells_of_Goto_Kakizaki_diabetes_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(09)01106-6 DB - PRIME DP - Unbound Medicine ER -