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A suppressor of a yeast splicing mutation (prp8-1) encodes a putative ATP-dependent RNA helicase.
Nature. 1991 Feb 21; 349(6311):715-7.Nat

Abstract

Five small nuclear RNAs (snRNAs) are required for nuclear pre-messenger RNA splicing: U1, U2, U4, U5 and U6. The yeast U1 and U2 snRNAs base-pair to the 5' splice site and branch-point sequences of introns respectively. The role of the U5 and U4/U6 small nuclear ribonucleoprotein particles (snRNPs) in splicing is not clear, though a catalytic role for the U6 snRNA has been proposed. Less is known about yeast splicing factors, but the availability of genetic techniques in Saccharomyces cerevisiae has led to the identification of mutants deficient in nuclear pre-mRNA splicing (prp2-prp27). Several PRP genes have now been cloned and their protein products characterized. The PRP8 protein is a component of the U5 snRNP and associates with the U4/U6 snRNAs/snRNP to form a multi-snRNP particle believed to be important for spliceosome assembly. We have isolated extragenic suppressors of the prp8-1 mutation of S. cerevisiae and present here the preliminary characterization of one of these suppressors, spp81. The predicted amino-acid sequence of the SPP81 protein shows extensive similarity to a recently identified family of proteins thought to possess ATP-dependent RNA helicase activity. The possible role of this putative helicase in nuclear pre-mRNA splicing is discussed.

Authors+Show Affiliations

Institute of Cell and Molecular Biology, University of Edinburgh, UK.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1996139

Citation

Jamieson, D J., et al. "A Suppressor of a Yeast Splicing Mutation (prp8-1) Encodes a Putative ATP-dependent RNA Helicase." Nature, vol. 349, no. 6311, 1991, pp. 715-7.
Jamieson DJ, Rahe B, Pringle J, et al. A suppressor of a yeast splicing mutation (prp8-1) encodes a putative ATP-dependent RNA helicase. Nature. 1991;349(6311):715-7.
Jamieson, D. J., Rahe, B., Pringle, J., & Beggs, J. D. (1991). A suppressor of a yeast splicing mutation (prp8-1) encodes a putative ATP-dependent RNA helicase. Nature, 349(6311), 715-7.
Jamieson DJ, et al. A Suppressor of a Yeast Splicing Mutation (prp8-1) Encodes a Putative ATP-dependent RNA Helicase. Nature. 1991 Feb 21;349(6311):715-7. PubMed PMID: 1996139.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A suppressor of a yeast splicing mutation (prp8-1) encodes a putative ATP-dependent RNA helicase. AU - Jamieson,D J, AU - Rahe,B, AU - Pringle,J, AU - Beggs,J D, PY - 1991/2/21/pubmed PY - 1991/2/21/medline PY - 1991/2/21/entrez SP - 715 EP - 7 JF - Nature JO - Nature VL - 349 IS - 6311 N2 - Five small nuclear RNAs (snRNAs) are required for nuclear pre-messenger RNA splicing: U1, U2, U4, U5 and U6. The yeast U1 and U2 snRNAs base-pair to the 5' splice site and branch-point sequences of introns respectively. The role of the U5 and U4/U6 small nuclear ribonucleoprotein particles (snRNPs) in splicing is not clear, though a catalytic role for the U6 snRNA has been proposed. Less is known about yeast splicing factors, but the availability of genetic techniques in Saccharomyces cerevisiae has led to the identification of mutants deficient in nuclear pre-mRNA splicing (prp2-prp27). Several PRP genes have now been cloned and their protein products characterized. The PRP8 protein is a component of the U5 snRNP and associates with the U4/U6 snRNAs/snRNP to form a multi-snRNP particle believed to be important for spliceosome assembly. We have isolated extragenic suppressors of the prp8-1 mutation of S. cerevisiae and present here the preliminary characterization of one of these suppressors, spp81. The predicted amino-acid sequence of the SPP81 protein shows extensive similarity to a recently identified family of proteins thought to possess ATP-dependent RNA helicase activity. The possible role of this putative helicase in nuclear pre-mRNA splicing is discussed. SN - 0028-0836 UR - https://www.unboundmedicine.com/medline/citation/1996139/A_suppressor_of_a_yeast_splicing_mutation__prp8_1__encodes_a_putative_ATP_dependent_RNA_helicase_ L2 - https://doi.org/10.1038/349715a0 DB - PRIME DP - Unbound Medicine ER -