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Emergence of H274Y oseltamivir-resistant A(H1N1) influenza viruses in Japan during the 2008-2009 season.

Abstract

BACKGROUND

A substantial increase in oseltamivir-resistant A(H1N1) influenza viruses was reported in Europe in late 2007.

OBJECTIVES

To monitor the antiviral susceptibility profile of human A(H1N1) influenza viruses in Japan during the 2007-2008 and 2008-2009 seasons.

STUDY DESIGN

Viruses were obtained from respiratory samples of patients with influenza collected in Japan between December 2007 and April 2008 (n=1046) and between December 2008 and April 2009 (n=1789). Oseltamivir resistance was determined by an H274Y-specific real-time PCR cycling probe assay and a neuraminidase inhibition assay. Amantadine resistance was assessed by sequencing the M2 gene. Sequencing of the hemagglutinin and NA genes was performed to infer phylogenetic relationships between different strains.

RESULTS

Three of 687 (0.4%) A(H1N1) viruses from the 2007-2008 season and 745 of 745 (100%) viruses from the 2008-2009 season carried the NA-H274Y substitution and demonstrated a >300-fold reduction in oseltamivir susceptibility. All oseltamivir-resistant viruses from the 2008-2009 season possessed an A193T substitution in the receptor-binding domain of the hemagglutinin. Amantadine resistance was detected in 431 of 687 (62.7%) and 0 of 745 (0.0%) of the A(H1N1) viruses from the 2007-2008 and 2008-2009 seasons, respectively.

CONCLUSIONS

A dramatic surge in oseltamivir-resistant A(H1N1) viruses possessing the NA-H274Y substitution was detected in Japan during the 2008-2009 season. The emergence of oseltamivir-resistant viruses was facilitated by mutations in the viral genome. Intensified surveillance, including phenotypic assays and sequencing of the hemagglutinin, neuraminidase, and M2 gene would allow monitoring of the spread and evolution of drug-resistant influenza virus variants.

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  • Authors+Show Affiliations

    ,

    Division of Public Health, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, 1-757 Asahimachi-Dori, Chuoku, Niigata City, Niigata Prefecture 951-8510, Japan. tbar@med.niigata-u.ac.jp

    , , , , , , , , ,

    Source

    MeSH

    Adolescent
    Adult
    Aged
    Aged, 80 and over
    Antiviral Agents
    Child
    Child, Preschool
    Drug Resistance, Viral
    Female
    Hemagglutinin Glycoproteins, Influenza Virus
    Humans
    Infant
    Influenza A Virus, H1N1 Subtype
    Influenza, Human
    Japan
    Male
    Middle Aged
    Neuraminidase
    Oseltamivir
    Phylogeny
    Point Mutation
    Sentinel Surveillance
    Viral Proteins

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    19962344

    Citation

    Baranovich, Tatiana, et al. "Emergence of H274Y Oseltamivir-resistant A(H1N1) Influenza Viruses in Japan During the 2008-2009 Season." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 47, no. 1, 2010, pp. 23-8.
    Baranovich T, Saito R, Suzuki Y, et al. Emergence of H274Y oseltamivir-resistant A(H1N1) influenza viruses in Japan during the 2008-2009 season. J Clin Virol. 2010;47(1):23-8.
    Baranovich, T., Saito, R., Suzuki, Y., Zaraket, H., Dapat, C., Caperig-Dapat, I., ... Suzuki, H. (2010). Emergence of H274Y oseltamivir-resistant A(H1N1) influenza viruses in Japan during the 2008-2009 season. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 47(1), pp. 23-8. doi:10.1016/j.jcv.2009.11.003.
    Baranovich T, et al. Emergence of H274Y Oseltamivir-resistant A(H1N1) Influenza Viruses in Japan During the 2008-2009 Season. J Clin Virol. 2010;47(1):23-8. PubMed PMID: 19962344.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Emergence of H274Y oseltamivir-resistant A(H1N1) influenza viruses in Japan during the 2008-2009 season. AU - Baranovich,Tatiana, AU - Saito,Reiko, AU - Suzuki,Yasushi, AU - Zaraket,Hassan, AU - Dapat,Clyde, AU - Caperig-Dapat,Isolde, AU - Oguma,Taeko, AU - Shabana,Iman Ibrahim, AU - Saito,Takehiko, AU - Suzuki,Hiroshi, AU - ,, Y1 - 2009/12/03/ PY - 2009/08/25/received PY - 2009/11/04/accepted PY - 2009/12/8/entrez PY - 2009/12/8/pubmed PY - 2010/5/5/medline SP - 23 EP - 8 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J. Clin. Virol. VL - 47 IS - 1 N2 - BACKGROUND: A substantial increase in oseltamivir-resistant A(H1N1) influenza viruses was reported in Europe in late 2007. OBJECTIVES: To monitor the antiviral susceptibility profile of human A(H1N1) influenza viruses in Japan during the 2007-2008 and 2008-2009 seasons. STUDY DESIGN: Viruses were obtained from respiratory samples of patients with influenza collected in Japan between December 2007 and April 2008 (n=1046) and between December 2008 and April 2009 (n=1789). Oseltamivir resistance was determined by an H274Y-specific real-time PCR cycling probe assay and a neuraminidase inhibition assay. Amantadine resistance was assessed by sequencing the M2 gene. Sequencing of the hemagglutinin and NA genes was performed to infer phylogenetic relationships between different strains. RESULTS: Three of 687 (0.4%) A(H1N1) viruses from the 2007-2008 season and 745 of 745 (100%) viruses from the 2008-2009 season carried the NA-H274Y substitution and demonstrated a >300-fold reduction in oseltamivir susceptibility. All oseltamivir-resistant viruses from the 2008-2009 season possessed an A193T substitution in the receptor-binding domain of the hemagglutinin. Amantadine resistance was detected in 431 of 687 (62.7%) and 0 of 745 (0.0%) of the A(H1N1) viruses from the 2007-2008 and 2008-2009 seasons, respectively. CONCLUSIONS: A dramatic surge in oseltamivir-resistant A(H1N1) viruses possessing the NA-H274Y substitution was detected in Japan during the 2008-2009 season. The emergence of oseltamivir-resistant viruses was facilitated by mutations in the viral genome. Intensified surveillance, including phenotypic assays and sequencing of the hemagglutinin, neuraminidase, and M2 gene would allow monitoring of the spread and evolution of drug-resistant influenza virus variants. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/19962344/Emergence_of_H274Y_oseltamivir_resistant_A_H1N1__influenza_viruses_in_Japan_during_the_2008_2009_season_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(09)00544-7 DB - PRIME DP - Unbound Medicine ER -