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Early diagnosis of dengue in travelers: comparison of a novel real-time RT-PCR, NS1 antigen detection and serology.
J Clin Virol. 2010 Jan; 47(1):49-53.JC

Abstract

BACKGROUND

The increased traveling to dengue endemic regions and the numerous epidemics have led to a rise in imported dengue. The laboratory diagnosis of acute dengue requires several types of tests and often paired samples are needed for obtaining reliable results. Although several diagnostic methods are available, proper comparative data on their performance are lacking.

OBJECTIVES

To compare the performance of novel methods including a novel pan-DENV real-time RT-PCR and a commercially available NS1 capture-EIA in regard to IgM detection for optimizing the early diagnosis of DENV in travelers.

STUDY DESIGN

A panel of 99 selected early phase serum samples of dengue patients was studied by real-time RT-PCR, NS1 antigen ELISA, IgM-EIA, IgG-IFA and cell culture virus isolation.

RESULTS

The novel real-time RT-PCR was shown specific and sensitive for detection of DENV-1-4 RNA and suitable for diagnostic use. The diagnostic rate using combination of RNA and IgM detection was 99% and using NS1 and IgM detection 95.9%. The results of RNA and NS1 antigen detection disagreed in 15.5% of samples that had only RNA or NS1 antigen detected.

CONCLUSIONS

The diagnostic rates of early samples are higher when either RNA or NS1 antigen detection is combined with IgM detection. Besides the differences in the RNA and NS1 detection assays, the observed discrepancy of results could suggest individual variation or differences in timing of these markers in patient serum.

Authors+Show Affiliations

Department of Virology, Haartman Institute, Faculty of Medicine, University of Helsinki, PO Box 21, Haartmaninkatu 3, FI-00014 University of Helsinki, Finland. eili.huhtamo@helsinki.fiNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19963435

Citation

Huhtamo, Eili, et al. "Early Diagnosis of Dengue in Travelers: Comparison of a Novel Real-time RT-PCR, NS1 Antigen Detection and Serology." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 47, no. 1, 2010, pp. 49-53.
Huhtamo E, Hasu E, Uzcátegui NY, et al. Early diagnosis of dengue in travelers: comparison of a novel real-time RT-PCR, NS1 antigen detection and serology. J Clin Virol. 2010;47(1):49-53.
Huhtamo, E., Hasu, E., Uzcátegui, N. Y., Erra, E., Nikkari, S., Kantele, A., Vapalahti, O., & Piiparinen, H. (2010). Early diagnosis of dengue in travelers: comparison of a novel real-time RT-PCR, NS1 antigen detection and serology. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 47(1), 49-53. https://doi.org/10.1016/j.jcv.2009.11.001
Huhtamo E, et al. Early Diagnosis of Dengue in Travelers: Comparison of a Novel Real-time RT-PCR, NS1 Antigen Detection and Serology. J Clin Virol. 2010;47(1):49-53. PubMed PMID: 19963435.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Early diagnosis of dengue in travelers: comparison of a novel real-time RT-PCR, NS1 antigen detection and serology. AU - Huhtamo,Eili, AU - Hasu,Essi, AU - Uzcátegui,Nathalie Y, AU - Erra,Elina, AU - Nikkari,Simo, AU - Kantele,Anu, AU - Vapalahti,Olli, AU - Piiparinen,Heli, Y1 - 2009/12/05/ PY - 2009/08/18/received PY - 2009/11/04/accepted PY - 2009/12/8/entrez PY - 2009/12/8/pubmed PY - 2010/5/5/medline SP - 49 EP - 53 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 47 IS - 1 N2 - BACKGROUND: The increased traveling to dengue endemic regions and the numerous epidemics have led to a rise in imported dengue. The laboratory diagnosis of acute dengue requires several types of tests and often paired samples are needed for obtaining reliable results. Although several diagnostic methods are available, proper comparative data on their performance are lacking. OBJECTIVES: To compare the performance of novel methods including a novel pan-DENV real-time RT-PCR and a commercially available NS1 capture-EIA in regard to IgM detection for optimizing the early diagnosis of DENV in travelers. STUDY DESIGN: A panel of 99 selected early phase serum samples of dengue patients was studied by real-time RT-PCR, NS1 antigen ELISA, IgM-EIA, IgG-IFA and cell culture virus isolation. RESULTS: The novel real-time RT-PCR was shown specific and sensitive for detection of DENV-1-4 RNA and suitable for diagnostic use. The diagnostic rate using combination of RNA and IgM detection was 99% and using NS1 and IgM detection 95.9%. The results of RNA and NS1 antigen detection disagreed in 15.5% of samples that had only RNA or NS1 antigen detected. CONCLUSIONS: The diagnostic rates of early samples are higher when either RNA or NS1 antigen detection is combined with IgM detection. Besides the differences in the RNA and NS1 detection assays, the observed discrepancy of results could suggest individual variation or differences in timing of these markers in patient serum. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/19963435/Early_diagnosis_of_dengue_in_travelers:_comparison_of_a_novel_real_time_RT_PCR_NS1_antigen_detection_and_serology_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(09)00542-3 DB - PRIME DP - Unbound Medicine ER -