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Fall and rise of satellite repeats in allopolyploids of Nicotiana over c. 5 million years.
New Phytol 2010; 186(1):148-60NP

Abstract

Allopolyploids represent natural experiments in which DNA sequences from different species are combined into a single nucleus and then coevolve, enabling us to follow the parental genomes, their interactions and evolution over time. Here, we examine the fate of satellite DNA over 5 million yr of divergence in plant genus Nicotiana (family Solanaceae). We isolated subtelomeric, tandemly repeated satellite DNA from Nicotiana diploid and allopolyploid species and analysed patterns of inheritance and divergence by sequence analysis, Southern blot hybridization and fluorescent in situ hybridization (FISH). We observed that parental satellite sequences redistribute around the genome in allopolyploids of Nicotiana section Polydicliae, formed c. 1 million yr ago (Mya), and that new satellite repeats evolved and amplified in section Repandae, which was formed c. 5 Mya. In some cases that process involved the complete replacement of parental satellite sequences. The rate of satellite repeat replacement is faster than theoretical predictions assuming the mechanism involved is unequal recombination and crossing-over. Instead we propose that this mechanism occurs with the deletion of large chromatin blocks and reamplification, perhaps via rolling circle replication.

Authors+Show Affiliations

Institute of Biophysics, Academy of Sciences of the Czech Republic, CZ-612 65 Brno, Czech Republic.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19968801

Citation

Koukalova, Blazena, et al. "Fall and Rise of Satellite Repeats in Allopolyploids of Nicotiana Over C. 5 Million Years." The New Phytologist, vol. 186, no. 1, 2010, pp. 148-60.
Koukalova B, Moraes AP, Renny-Byfield S, et al. Fall and rise of satellite repeats in allopolyploids of Nicotiana over c. 5 million years. New Phytol. 2010;186(1):148-60.
Koukalova, B., Moraes, A. P., Renny-Byfield, S., Matyasek, R., Leitch, A. R., & Kovarik, A. (2010). Fall and rise of satellite repeats in allopolyploids of Nicotiana over c. 5 million years. The New Phytologist, 186(1), pp. 148-60. doi:10.1111/j.1469-8137.2009.03101.x.
Koukalova B, et al. Fall and Rise of Satellite Repeats in Allopolyploids of Nicotiana Over C. 5 Million Years. New Phytol. 2010;186(1):148-60. PubMed PMID: 19968801.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fall and rise of satellite repeats in allopolyploids of Nicotiana over c. 5 million years. AU - Koukalova,Blazena, AU - Moraes,Ana Paula, AU - Renny-Byfield,Simon, AU - Matyasek,Roman, AU - Leitch,Andrew Rowland, AU - Kovarik,Ales, Y1 - 2009/12/03/ PY - 2009/12/9/entrez PY - 2009/12/9/pubmed PY - 2010/7/9/medline SP - 148 EP - 60 JF - The New phytologist JO - New Phytol. VL - 186 IS - 1 N2 - Allopolyploids represent natural experiments in which DNA sequences from different species are combined into a single nucleus and then coevolve, enabling us to follow the parental genomes, their interactions and evolution over time. Here, we examine the fate of satellite DNA over 5 million yr of divergence in plant genus Nicotiana (family Solanaceae). We isolated subtelomeric, tandemly repeated satellite DNA from Nicotiana diploid and allopolyploid species and analysed patterns of inheritance and divergence by sequence analysis, Southern blot hybridization and fluorescent in situ hybridization (FISH). We observed that parental satellite sequences redistribute around the genome in allopolyploids of Nicotiana section Polydicliae, formed c. 1 million yr ago (Mya), and that new satellite repeats evolved and amplified in section Repandae, which was formed c. 5 Mya. In some cases that process involved the complete replacement of parental satellite sequences. The rate of satellite repeat replacement is faster than theoretical predictions assuming the mechanism involved is unequal recombination and crossing-over. Instead we propose that this mechanism occurs with the deletion of large chromatin blocks and reamplification, perhaps via rolling circle replication. SN - 1469-8137 UR - https://www.unboundmedicine.com/medline/citation/19968801/Fall_and_rise_of_satellite_repeats_in_allopolyploids_of_Nicotiana_over_c__5_million_years_ L2 - https://doi.org/10.1111/j.1469-8137.2009.03101.x DB - PRIME DP - Unbound Medicine ER -