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Systemic administration of 3-nitropropionic acid points out a different role for active caspase-3 in neurons and astrocytes.
Neurochem Int. 2010 Feb; 56(3):443-50.NI

Abstract

The intraperitoneal administration of 3-nitropropionic acid, which is commonly used to induce toxicity models of Huntington's disease in experimental animals, produces severe brain injury in the lateral part of the striatum. We studied the presence of active caspase-3 in neurons and astrocytes from brains of rats treated with 3-nitropropionic acid following a subacute administration protocol. Active caspase-3 was almost absent in the core of the striatal lesion. However, it was expressed, albeit weakly, in the neurons present in the rim of the lesion. In cortex and non-injured striatal areas, and in the cortex and striatum of control animals, active caspase-3 staining was widely distributed and vivid, but localized in the cell bodies of astrocytes rather than in neurons. In treated animals, some of the active caspase-3 positive neurons localized in the rim of the lesion were also positive for TUNEL staining. This indicates the presence of a caspase-mediated apoptotic process. TUNEL was not present in control animals or in the astrocytes of treated animals. Thus, the presence of active caspase-3 in astrocytes may be merely constitutive.

Authors+Show Affiliations

Departament de Fisiologia, Facultat de Farmàcia, Universitat de Barcelona, Av Joan XXIII s/n, 08028 Barcelona, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19969037

Citation

Duran-Vilaregut, Joaquim, et al. "Systemic Administration of 3-nitropropionic Acid Points Out a Different Role for Active Caspase-3 in Neurons and Astrocytes." Neurochemistry International, vol. 56, no. 3, 2010, pp. 443-50.
Duran-Vilaregut J, Del Valle J, Manich G, et al. Systemic administration of 3-nitropropionic acid points out a different role for active caspase-3 in neurons and astrocytes. Neurochem Int. 2010;56(3):443-50.
Duran-Vilaregut, J., Del Valle, J., Manich, G., Junyent, F., Camins, A., Pallàs, M., Pelegrí, C., & Vilaplana, J. (2010). Systemic administration of 3-nitropropionic acid points out a different role for active caspase-3 in neurons and astrocytes. Neurochemistry International, 56(3), 443-50. https://doi.org/10.1016/j.neuint.2009.12.001
Duran-Vilaregut J, et al. Systemic Administration of 3-nitropropionic Acid Points Out a Different Role for Active Caspase-3 in Neurons and Astrocytes. Neurochem Int. 2010;56(3):443-50. PubMed PMID: 19969037.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Systemic administration of 3-nitropropionic acid points out a different role for active caspase-3 in neurons and astrocytes. AU - Duran-Vilaregut,Joaquim, AU - Del Valle,Jaume, AU - Manich,Gemma, AU - Junyent,Fèlix, AU - Camins,Antoni, AU - Pallàs,Mercè, AU - Pelegrí,Carme, AU - Vilaplana,Jordi, Y1 - 2009/12/05/ PY - 2009/09/03/received PY - 2009/11/27/revised PY - 2009/12/01/accepted PY - 2009/12/9/entrez PY - 2009/12/9/pubmed PY - 2010/6/9/medline SP - 443 EP - 50 JF - Neurochemistry international JO - Neurochem Int VL - 56 IS - 3 N2 - The intraperitoneal administration of 3-nitropropionic acid, which is commonly used to induce toxicity models of Huntington's disease in experimental animals, produces severe brain injury in the lateral part of the striatum. We studied the presence of active caspase-3 in neurons and astrocytes from brains of rats treated with 3-nitropropionic acid following a subacute administration protocol. Active caspase-3 was almost absent in the core of the striatal lesion. However, it was expressed, albeit weakly, in the neurons present in the rim of the lesion. In cortex and non-injured striatal areas, and in the cortex and striatum of control animals, active caspase-3 staining was widely distributed and vivid, but localized in the cell bodies of astrocytes rather than in neurons. In treated animals, some of the active caspase-3 positive neurons localized in the rim of the lesion were also positive for TUNEL staining. This indicates the presence of a caspase-mediated apoptotic process. TUNEL was not present in control animals or in the astrocytes of treated animals. Thus, the presence of active caspase-3 in astrocytes may be merely constitutive. SN - 1872-9754 UR - https://www.unboundmedicine.com/medline/citation/19969037/Systemic_administration_of_3_nitropropionic_acid_points_out_a_different_role_for_active_caspase_3_in_neurons_and_astrocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0197-0186(09)00325-8 DB - PRIME DP - Unbound Medicine ER -