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Evaluation of IgM antibody capture enzyme-linked immunosorbent assay kits for detection of IgM against Japanese encephalitis virus in cerebrospinal fluid samples.
Am J Trop Med Hyg. 2009 Dec; 81(6):1144-50.AJ

Abstract

Infection with Japanese encephalitis virus (JEV) is a major public health problem in Asia. Detection of JEV-specific IgM in serum and cerebrospinal fluid (CSF) by the IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) is currently the most widely used diagnostic method to detect JEV infection. Because of the possible presence of IgM cross-reactivity with other flaviviruses in serum and the high ratio of inapparent-to-apparent JEV infections, a positive result in serum only suggests a recent infection and not necessarily an encephalitic illness caused by JEV. Consequently, detection of JEV-specific IgM in CSF assumes great diagnostic relevance. We evaluated two commercial JEV MAC-ELISA kits using 60 CSF samples obtained from patients with acute encephalitis syndrome. The Panbio and XCyton kits had sensitivities of 65-80% and 95% and specificities of 90% and 97.5%, respectively. Performance information on these commercial JEV MAC-ELISA kits for CSF should assist in laboratory-based JE surveillance programs.

Authors+Show Affiliations

Department of Neurovirology, National Institute of Mental Health and Neuro Sciences, Bangalore, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

19996450

Citation

Ravi, Vasanthapuram, et al. "Evaluation of IgM Antibody Capture Enzyme-linked Immunosorbent Assay Kits for Detection of IgM Against Japanese Encephalitis Virus in Cerebrospinal Fluid Samples." The American Journal of Tropical Medicine and Hygiene, vol. 81, no. 6, 2009, pp. 1144-50.
Ravi V, Robinson JS, Russell BJ, et al. Evaluation of IgM antibody capture enzyme-linked immunosorbent assay kits for detection of IgM against Japanese encephalitis virus in cerebrospinal fluid samples. Am J Trop Med Hyg. 2009;81(6):1144-50.
Ravi, V., Robinson, J. S., Russell, B. J., Desai, A., Ramamurty, N., Featherstone, D., & Johnson, B. W. (2009). Evaluation of IgM antibody capture enzyme-linked immunosorbent assay kits for detection of IgM against Japanese encephalitis virus in cerebrospinal fluid samples. The American Journal of Tropical Medicine and Hygiene, 81(6), 1144-50. https://doi.org/10.4269/ajtmh.2009.09-0144
Ravi V, et al. Evaluation of IgM Antibody Capture Enzyme-linked Immunosorbent Assay Kits for Detection of IgM Against Japanese Encephalitis Virus in Cerebrospinal Fluid Samples. Am J Trop Med Hyg. 2009;81(6):1144-50. PubMed PMID: 19996450.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of IgM antibody capture enzyme-linked immunosorbent assay kits for detection of IgM against Japanese encephalitis virus in cerebrospinal fluid samples. AU - Ravi,Vasanthapuram, AU - Robinson,Jaimie S, AU - Russell,Brandy J, AU - Desai,Anita, AU - Ramamurty,Nalini, AU - Featherstone,David, AU - Johnson,Barbara W, PY - 2009/12/10/entrez PY - 2009/12/10/pubmed PY - 2010/1/6/medline SP - 1144 EP - 50 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 81 IS - 6 N2 - Infection with Japanese encephalitis virus (JEV) is a major public health problem in Asia. Detection of JEV-specific IgM in serum and cerebrospinal fluid (CSF) by the IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) is currently the most widely used diagnostic method to detect JEV infection. Because of the possible presence of IgM cross-reactivity with other flaviviruses in serum and the high ratio of inapparent-to-apparent JEV infections, a positive result in serum only suggests a recent infection and not necessarily an encephalitic illness caused by JEV. Consequently, detection of JEV-specific IgM in CSF assumes great diagnostic relevance. We evaluated two commercial JEV MAC-ELISA kits using 60 CSF samples obtained from patients with acute encephalitis syndrome. The Panbio and XCyton kits had sensitivities of 65-80% and 95% and specificities of 90% and 97.5%, respectively. Performance information on these commercial JEV MAC-ELISA kits for CSF should assist in laboratory-based JE surveillance programs. SN - 1476-1645 UR - https://www.unboundmedicine.com/medline/citation/19996450/Evaluation_of_IgM_antibody_capture_enzyme_linked_immunosorbent_assay_kits_for_detection_of_IgM_against_Japanese_encephalitis_virus_in_cerebrospinal_fluid_samples_ L2 - https://ajtmh.org/doi/10.4269/ajtmh.2009.09-0144 DB - PRIME DP - Unbound Medicine ER -