Tags

Type your tag names separated by a space and hit enter

Electrochemical evaluation and determination of antiretroviral drug fosamprenavir using boron-doped diamond and glassy carbon electrodes.
Anal Bioanal Chem. 2010 May; 397(1):189-203.AB

Abstract

Fosamprenavir is a pro-drug of the antiretroviral protease inhibitor amprenavir and is oxidizable at solid electrodes. The anodic oxidation behavior of fosamprenavir was investigated using cyclic and linear sweep voltammetry at boron-doped diamond and glassy carbon electrodes. In cyclic voltammetry, depending on pH values, fosamprenavir showed one sharp irreversible oxidation peak or wave depending on the working electrode. The mechanism of the oxidation process was discussed. The voltammetric study of some model compounds allowed elucidation of the possible oxidation mechanism of fosamprenavir. The aim of this study was to determine fosamprenavir levels in pharmaceutical formulations and biological samples by means of electrochemical methods. Using the sharp oxidation response, two voltammetric methods were described for the determination of fosamprenavir by differential pulse and square-wave voltammetry at the boron-doped diamond and glassy carbon electrodes. These two voltammetric techniques are 0.1 M H(2)SO(4) and phosphate buffer at pH 2.0 which allow quantitation over a 4 x 10(-6) to 8 x 10(-5) M range using boron-doped diamond and a 1 x 10(-5) to 1 x 10(-4) M range using glassy carbon electrodes, respectively, in supporting electrolyte. All necessary validation parameters were investigated and calculated. These methods were successfully applied for the analysis of fosamprenavir pharmaceutical dosage forms, human serum and urine samples. The standard addition method was used in biological media using boron-doped diamond electrode. No electroactive interferences from the tablet excipients or endogenous substances from biological material were found. The results were statistically compared with those obtained through an established HPLC-UV technique; no significant differences were found between the voltammetric and HPLC methods.

Authors+Show Affiliations

Faculty of Pharmacy, Department of Analytical Chemistry, Ankara University, Ankara, 06100, Turkey. Faculty of Art & Science, Department of Chemistry, Hitit University, Corum, 19040, Turkey.Faculty of Pharmacy, Department of Analytical Chemistry, Ankara University, Ankara, 06100, Turkey. ozkan@pharmacy.ankara.edu.tr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19998025

Citation

Gumustas, Mehmet, and Sibel A. Ozkan. "Electrochemical Evaluation and Determination of Antiretroviral Drug Fosamprenavir Using Boron-doped Diamond and Glassy Carbon Electrodes." Analytical and Bioanalytical Chemistry, vol. 397, no. 1, 2010, pp. 189-203.
Gumustas M, Ozkan SA. Electrochemical evaluation and determination of antiretroviral drug fosamprenavir using boron-doped diamond and glassy carbon electrodes. Anal Bioanal Chem. 2010;397(1):189-203.
Gumustas, M., & Ozkan, S. A. (2010). Electrochemical evaluation and determination of antiretroviral drug fosamprenavir using boron-doped diamond and glassy carbon electrodes. Analytical and Bioanalytical Chemistry, 397(1), 189-203. https://doi.org/10.1007/s00216-009-3334-3
Gumustas M, Ozkan SA. Electrochemical Evaluation and Determination of Antiretroviral Drug Fosamprenavir Using Boron-doped Diamond and Glassy Carbon Electrodes. Anal Bioanal Chem. 2010;397(1):189-203. PubMed PMID: 19998025.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemical evaluation and determination of antiretroviral drug fosamprenavir using boron-doped diamond and glassy carbon electrodes. AU - Gumustas,Mehmet, AU - Ozkan,Sibel A, Y1 - 2009/12/10/ PY - 2009/10/13/received PY - 2009/11/19/accepted PY - 2009/11/19/revised PY - 2009/12/10/entrez PY - 2009/12/10/pubmed PY - 2010/7/22/medline SP - 189 EP - 203 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 397 IS - 1 N2 - Fosamprenavir is a pro-drug of the antiretroviral protease inhibitor amprenavir and is oxidizable at solid electrodes. The anodic oxidation behavior of fosamprenavir was investigated using cyclic and linear sweep voltammetry at boron-doped diamond and glassy carbon electrodes. In cyclic voltammetry, depending on pH values, fosamprenavir showed one sharp irreversible oxidation peak or wave depending on the working electrode. The mechanism of the oxidation process was discussed. The voltammetric study of some model compounds allowed elucidation of the possible oxidation mechanism of fosamprenavir. The aim of this study was to determine fosamprenavir levels in pharmaceutical formulations and biological samples by means of electrochemical methods. Using the sharp oxidation response, two voltammetric methods were described for the determination of fosamprenavir by differential pulse and square-wave voltammetry at the boron-doped diamond and glassy carbon electrodes. These two voltammetric techniques are 0.1 M H(2)SO(4) and phosphate buffer at pH 2.0 which allow quantitation over a 4 x 10(-6) to 8 x 10(-5) M range using boron-doped diamond and a 1 x 10(-5) to 1 x 10(-4) M range using glassy carbon electrodes, respectively, in supporting electrolyte. All necessary validation parameters were investigated and calculated. These methods were successfully applied for the analysis of fosamprenavir pharmaceutical dosage forms, human serum and urine samples. The standard addition method was used in biological media using boron-doped diamond electrode. No electroactive interferences from the tablet excipients or endogenous substances from biological material were found. The results were statistically compared with those obtained through an established HPLC-UV technique; no significant differences were found between the voltammetric and HPLC methods. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/19998025/Electrochemical_evaluation_and_determination_of_antiretroviral_drug_fosamprenavir_using_boron_doped_diamond_and_glassy_carbon_electrodes_ L2 - https://dx.doi.org/10.1007/s00216-009-3334-3 DB - PRIME DP - Unbound Medicine ER -