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Sensitive bifunctional aptamer-based electrochemical biosensor for small molecules and protein.
Anal Chem. 2009 Dec 15; 81(24):9972-8.AC

Abstract

In this paper, a bifunctional electrochemical biosensor for highly sensitive detection of small molecule (adenosine) or protein (lysozyme) was developed. Two aptamer units for adenosine and lysozyme were immobilized on the gold electrode by the formation of DNA/DNA duplex. The detection of adenosine or lysozyme could be carried out by virtue of switching structures of aptamers from DNA/DNA duplex to DNA/target complex. The change of the interfacial feature of the electrode was characterized by cyclic voltammertic (CV) response of surface-bound [Ru(NH(3))(6)](3+). On the other hand, DNA functionalized Au nanoparticles (DNA-AuNPs) were used to enhance the sensitivity of the aptasensor because DNA-AuNPs modified interface could load more [Ru(NH(3))(6)](3+) cations. Thus, the assembly of two aptamer-contained DNA strands integrated with the DNA-AuNPs amplification not only improves the sensitivity of the electrochemical aptasensor but also presents a simple and general model for bifunctional aptasensor. The proposed aptasensor has low detection limit (0.02 nM for adenosine and 0.01 microg mL(-1) for lysozyme) and exhibits several advantages such as high sensitivity and bifunctional recognition.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Deng C
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.
Chen J
No affiliation info available
Nie L
No affiliation info available
Nie Z
No affiliation info available
Yao S
No affiliation info available

MeSH

AdenosineAptamers, NucleotideBiosensing TechniquesElectrochemistryElectrodesGoldMetal NanoparticlesMuramidaseOrganometallic CompoundsSensitivity and SpecificitySurface Properties

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20000640

Citation

Deng, Chunyan, et al. "Sensitive Bifunctional Aptamer-based Electrochemical Biosensor for Small Molecules and Protein." Analytical Chemistry, vol. 81, no. 24, 2009, pp. 9972-8.
Deng C, Chen J, Nie L, et al. Sensitive bifunctional aptamer-based electrochemical biosensor for small molecules and protein. Anal Chem. 2009;81(24):9972-8.
Deng, C., Chen, J., Nie, L., Nie, Z., & Yao, S. (2009). Sensitive bifunctional aptamer-based electrochemical biosensor for small molecules and protein. Analytical Chemistry, 81(24), 9972-8. https://doi.org/10.1021/ac901727z
Deng C, et al. Sensitive Bifunctional Aptamer-based Electrochemical Biosensor for Small Molecules and Protein. Anal Chem. 2009 Dec 15;81(24):9972-8. PubMed PMID: 20000640.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitive bifunctional aptamer-based electrochemical biosensor for small molecules and protein. AU - Deng,Chunyan, AU - Chen,Jinhua, AU - Nie,Lihua, AU - Nie,Zhou, AU - Yao,Shouzhuo, PY - 2009/12/17/entrez PY - 2009/12/17/pubmed PY - 2010/2/25/medline SP - 9972 EP - 8 JF - Analytical chemistry JO - Anal Chem VL - 81 IS - 24 N2 - In this paper, a bifunctional electrochemical biosensor for highly sensitive detection of small molecule (adenosine) or protein (lysozyme) was developed. Two aptamer units for adenosine and lysozyme were immobilized on the gold electrode by the formation of DNA/DNA duplex. The detection of adenosine or lysozyme could be carried out by virtue of switching structures of aptamers from DNA/DNA duplex to DNA/target complex. The change of the interfacial feature of the electrode was characterized by cyclic voltammertic (CV) response of surface-bound [Ru(NH(3))(6)](3+). On the other hand, DNA functionalized Au nanoparticles (DNA-AuNPs) were used to enhance the sensitivity of the aptasensor because DNA-AuNPs modified interface could load more [Ru(NH(3))(6)](3+) cations. Thus, the assembly of two aptamer-contained DNA strands integrated with the DNA-AuNPs amplification not only improves the sensitivity of the electrochemical aptasensor but also presents a simple and general model for bifunctional aptasensor. The proposed aptasensor has low detection limit (0.02 nM for adenosine and 0.01 microg mL(-1) for lysozyme) and exhibits several advantages such as high sensitivity and bifunctional recognition. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/20000640/Sensitive_bifunctional_aptamer_based_electrochemical_biosensor_for_small_molecules_and_protein_ L2 - https://doi.org/10.1021/ac901727z DB - PRIME DP - Unbound Medicine ER -