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Analysis of amino acids and biogenic amines in breast cancer cells by capillary electrophoresis using polymer solutions containing sodium dodecyl sulfate.
J Chromatogr A. 2010 Jan 22; 1217(4):582-7.JC

Abstract

We describe simultaneous analysis of naphthalene-2,3-dicarboxaldehyde (NDA)-amino acid and NDA-biogenic amine derivatives by CE in conjunction with light-emitting diode-induced fluorescence detection using poly(ethylene oxide) (PEO) solutions containing sodium dodecyl sulfate (SDS). After sample injection, via EOF 0.1% PEO prepared in 100mM TB solution (pH 9.0) containing 30 mM SDS entered a capillary filled with 0.5M TB solution (pH 10.2) containing 40 mM SDS. Under this condition, 14 NDA-amino acid and NDA-amine derivatives were separated within 16 min, with high efficiency ((1.0-3.2)x10(5) theoretical plates) and sensitivity (LODs at S/N=3 ranging from 2.06 to 19.17 nM). In the presence of SDS and PEO, analytes adsorption on the capillary wall was suppressed, leading to high efficiency and reproducibility. The intraday analysis RSD values (n=3) of the mobilities for the analytes are less than 0.52%. We have validated the practicality of this approach by quantitative determination of 9 amino acids in breast cancer cells (MCF-7) and 10 amino acids in normal epithelial cells (H184B5F5/M10). The concentrations of Tau and Gln in the MCF-7 cells were different than those in the H184B5F5/M10 cells, respectively. Our results show the potential of this approach for cancer study.

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Authors+Show Affiliations

Kao YY
Department of Chemistry, National Taiwan University, 1 Section 4, Roosevelt Road, Taipei 106, Taiwan.
Liu KT
No affiliation info available
Huang MF
No affiliation info available
Chiu TC
No affiliation info available
Chang HT
No affiliation info available

MeSH

Amino AcidsBiogenic AminesBreast NeoplasmsCell Line, TumorElectrophoresis, CapillaryFemaleFluorescenceHumansLimit of DetectionNaphthalenesPolyethylene GlycolsReproducibility of ResultsSodium Dodecyl Sulfate

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

20004398

Citation

Kao, Yu-Yen, et al. "Analysis of Amino Acids and Biogenic Amines in Breast Cancer Cells By Capillary Electrophoresis Using Polymer Solutions Containing Sodium Dodecyl Sulfate." Journal of Chromatography. A, vol. 1217, no. 4, 2010, pp. 582-7.
Kao YY, Liu KT, Huang MF, et al. Analysis of amino acids and biogenic amines in breast cancer cells by capillary electrophoresis using polymer solutions containing sodium dodecyl sulfate. J Chromatogr A. 2010;1217(4):582-7.
Kao, Y. Y., Liu, K. T., Huang, M. F., Chiu, T. C., & Chang, H. T. (2010). Analysis of amino acids and biogenic amines in breast cancer cells by capillary electrophoresis using polymer solutions containing sodium dodecyl sulfate. Journal of Chromatography. A, 1217(4), 582-7. https://doi.org/10.1016/j.chroma.2009.11.069
Kao YY, et al. Analysis of Amino Acids and Biogenic Amines in Breast Cancer Cells By Capillary Electrophoresis Using Polymer Solutions Containing Sodium Dodecyl Sulfate. J Chromatogr A. 2010 Jan 22;1217(4):582-7. PubMed PMID: 20004398.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of amino acids and biogenic amines in breast cancer cells by capillary electrophoresis using polymer solutions containing sodium dodecyl sulfate. AU - Kao,Yu-Yen, AU - Liu,Kung-Tien, AU - Huang,Ming-Feng, AU - Chiu,Tai-Chia, AU - Chang,Huan-Tsung, Y1 - 2009/12/03/ PY - 2009/09/11/received PY - 2009/11/20/revised PY - 2009/11/23/accepted PY - 2009/12/17/entrez PY - 2009/12/17/pubmed PY - 2010/4/7/medline SP - 582 EP - 7 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1217 IS - 4 N2 - We describe simultaneous analysis of naphthalene-2,3-dicarboxaldehyde (NDA)-amino acid and NDA-biogenic amine derivatives by CE in conjunction with light-emitting diode-induced fluorescence detection using poly(ethylene oxide) (PEO) solutions containing sodium dodecyl sulfate (SDS). After sample injection, via EOF 0.1% PEO prepared in 100mM TB solution (pH 9.0) containing 30 mM SDS entered a capillary filled with 0.5M TB solution (pH 10.2) containing 40 mM SDS. Under this condition, 14 NDA-amino acid and NDA-amine derivatives were separated within 16 min, with high efficiency ((1.0-3.2)x10(5) theoretical plates) and sensitivity (LODs at S/N=3 ranging from 2.06 to 19.17 nM). In the presence of SDS and PEO, analytes adsorption on the capillary wall was suppressed, leading to high efficiency and reproducibility. The intraday analysis RSD values (n=3) of the mobilities for the analytes are less than 0.52%. We have validated the practicality of this approach by quantitative determination of 9 amino acids in breast cancer cells (MCF-7) and 10 amino acids in normal epithelial cells (H184B5F5/M10). The concentrations of Tau and Gln in the MCF-7 cells were different than those in the H184B5F5/M10 cells, respectively. Our results show the potential of this approach for cancer study. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/20004398/Analysis_of_amino_acids_and_biogenic_amines_in_breast_cancer_cells_by_capillary_electrophoresis_using_polymer_solutions_containing_sodium_dodecyl_sulfate_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)01756-7 DB - PRIME DP - Unbound Medicine ER -