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Preliminary molecular analysis of Clostridium difficile isolates from healthy horses in northern Italy.
Comp Immunol Microbiol Infect Dis. 2010 Dec; 33(6):e25-9.CI

Abstract

Clostridium difficile, associated with a wide spectrum of diseases in humans, as well as in several animal species, is an important cause of colitis in adult horses and foals. The aim of this study was to investigate by toxin gene profile and PCR-ribotyping the molecular characteristics of 14 C. difficile strains isolated from 42 faeces of healthy horses. Both toxin genes, tcdA and tcdB, were present in only 1 isolate (7.1%). Six isolates (42.9%) demonstrated tcdA-/tcdB+ genotype, and seven isolates (50.0%) were tcdA-/tcdB-. All strains were binary toxin genes negative (cdtA-/cdtB-). The PCR-positive strains, except for the tcdA+/tcdB+ isolate, tested negative for, in vitro, A and/or B toxins production by EIA. Eleven distinct ribotypes were observed. In conclusion, C. difficile can be present in the normal intestinal flora of healthy adult horses, in addition to foals. These animals could therefore play an important role as potential reservoirs of toxigenic strains.

Authors+Show Affiliations

Department of Animal Health, Faculty of Veterinary Medicine, University of Parma, Via del Taglio no. 8, 43100 Parma, Italy. mariacristina.ossiprandi@unipr.itNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20004974

Citation

Ossiprandi, Maria Cristina, et al. "Preliminary Molecular Analysis of Clostridium Difficile Isolates From Healthy Horses in Northern Italy." Comparative Immunology, Microbiology and Infectious Diseases, vol. 33, no. 6, 2010, pp. e25-9.
Ossiprandi MC, Buttrini M, Bottarelli E, et al. Preliminary molecular analysis of Clostridium difficile isolates from healthy horses in northern Italy. Comp Immunol Microbiol Infect Dis. 2010;33(6):e25-9.
Ossiprandi, M. C., Buttrini, M., Bottarelli, E., & Zerbini, L. (2010). Preliminary molecular analysis of Clostridium difficile isolates from healthy horses in northern Italy. Comparative Immunology, Microbiology and Infectious Diseases, 33(6), e25-9. https://doi.org/10.1016/j.cimid.2009.10.008
Ossiprandi MC, et al. Preliminary Molecular Analysis of Clostridium Difficile Isolates From Healthy Horses in Northern Italy. Comp Immunol Microbiol Infect Dis. 2010;33(6):e25-9. PubMed PMID: 20004974.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preliminary molecular analysis of Clostridium difficile isolates from healthy horses in northern Italy. AU - Ossiprandi,Maria Cristina, AU - Buttrini,Mirko, AU - Bottarelli,Ezio, AU - Zerbini,Laura, Y1 - 2009/12/11/ PY - 2009/10/21/accepted PY - 2009/12/17/entrez PY - 2009/12/17/pubmed PY - 2011/5/26/medline SP - e25 EP - 9 JF - Comparative immunology, microbiology and infectious diseases JO - Comp Immunol Microbiol Infect Dis VL - 33 IS - 6 N2 - Clostridium difficile, associated with a wide spectrum of diseases in humans, as well as in several animal species, is an important cause of colitis in adult horses and foals. The aim of this study was to investigate by toxin gene profile and PCR-ribotyping the molecular characteristics of 14 C. difficile strains isolated from 42 faeces of healthy horses. Both toxin genes, tcdA and tcdB, were present in only 1 isolate (7.1%). Six isolates (42.9%) demonstrated tcdA-/tcdB+ genotype, and seven isolates (50.0%) were tcdA-/tcdB-. All strains were binary toxin genes negative (cdtA-/cdtB-). The PCR-positive strains, except for the tcdA+/tcdB+ isolate, tested negative for, in vitro, A and/or B toxins production by EIA. Eleven distinct ribotypes were observed. In conclusion, C. difficile can be present in the normal intestinal flora of healthy adult horses, in addition to foals. These animals could therefore play an important role as potential reservoirs of toxigenic strains. SN - 1878-1667 UR - https://www.unboundmedicine.com/medline/citation/20004974/Preliminary_molecular_analysis_of_Clostridium_difficile_isolates_from_healthy_horses_in_northern_Italy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-9571(09)00060-5 DB - PRIME DP - Unbound Medicine ER -