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Succinimidyl oleate, established inhibitor of CD36/FAT translocase inhibits complex III of mitochondrial respiratory chain.
Biochem Biophys Res Commun. 2010 Jan 15; 391(3):1348-51.BB

Abstract

The functional role of CD36 protein detected in mitochondrial fractions in long chain fatty acid (LCFA) oxidation is unclear due to conflicting results obtained in Cd36 knockout mice and experiments using sulfo-N-succinimidyl oleate (SSO) for inhibition of CD36 mediated LCFA transport. We investigated effect of SSO on mitochondrial respiration and found that SSO substantially inhibits not only LCFA oxidation, but also oxidation of flavoprotein- and NADH-dependent substrates and generation of mitochondrial membrane potential. Experiments in rat liver, heart and kidney mitochondria demonstrated a direct effect on mitochondrial respiratory chain with the most pronounced inhibition of the complex III (IC(50) 4microM SSO). The results presented here show that SSO is a potent and irreversible inhibitor of mitochondrial respiratory chain.

Authors+Show Affiliations

Institute of Physiology and Center for Applied Genomics, Academy of Sciences of the Czech Republic, Vídenská 1083, 142 20 Prague, Czech Republic.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20006584

Citation

Drahota, Zdenek, et al. "Succinimidyl Oleate, Established Inhibitor of CD36/FAT Translocase Inhibits Complex III of Mitochondrial Respiratory Chain." Biochemical and Biophysical Research Communications, vol. 391, no. 3, 2010, pp. 1348-51.
Drahota Z, Vrbacký M, Nůsková H, et al. Succinimidyl oleate, established inhibitor of CD36/FAT translocase inhibits complex III of mitochondrial respiratory chain. Biochem Biophys Res Commun. 2010;391(3):1348-51.
Drahota, Z., Vrbacký, M., Nůsková, H., Kazdová, L., Zídek, V., Landa, V., Pravenec, M., & Houstek, J. (2010). Succinimidyl oleate, established inhibitor of CD36/FAT translocase inhibits complex III of mitochondrial respiratory chain. Biochemical and Biophysical Research Communications, 391(3), 1348-51. https://doi.org/10.1016/j.bbrc.2009.12.050
Drahota Z, et al. Succinimidyl Oleate, Established Inhibitor of CD36/FAT Translocase Inhibits Complex III of Mitochondrial Respiratory Chain. Biochem Biophys Res Commun. 2010 Jan 15;391(3):1348-51. PubMed PMID: 20006584.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Succinimidyl oleate, established inhibitor of CD36/FAT translocase inhibits complex III of mitochondrial respiratory chain. AU - Drahota,Zdenek, AU - Vrbacký,Marek, AU - Nůsková,Hana, AU - Kazdová,Ludmila, AU - Zídek,Václav, AU - Landa,Vladimír, AU - Pravenec,Michal, AU - Houstek,Josef, Y1 - 2009/12/16/ PY - 2009/11/26/received PY - 2009/12/09/accepted PY - 2009/12/17/entrez PY - 2009/12/17/pubmed PY - 2010/2/23/medline SP - 1348 EP - 51 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 391 IS - 3 N2 - The functional role of CD36 protein detected in mitochondrial fractions in long chain fatty acid (LCFA) oxidation is unclear due to conflicting results obtained in Cd36 knockout mice and experiments using sulfo-N-succinimidyl oleate (SSO) for inhibition of CD36 mediated LCFA transport. We investigated effect of SSO on mitochondrial respiration and found that SSO substantially inhibits not only LCFA oxidation, but also oxidation of flavoprotein- and NADH-dependent substrates and generation of mitochondrial membrane potential. Experiments in rat liver, heart and kidney mitochondria demonstrated a direct effect on mitochondrial respiratory chain with the most pronounced inhibition of the complex III (IC(50) 4microM SSO). The results presented here show that SSO is a potent and irreversible inhibitor of mitochondrial respiratory chain. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/20006584/Succinimidyl_oleate_established_inhibitor_of_CD36/FAT_translocase_inhibits_complex_III_of_mitochondrial_respiratory_chain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(09)02423-1 DB - PRIME DP - Unbound Medicine ER -