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Non-invasive diagnosis of endometriosis based on a combined analysis of six plasma biomarkers.
Hum Reprod. 2010 Mar; 25(3):654-64.HR

Abstract

BACKGROUND

Lack of a non-invasive diagnostic test contributes to the long delay between onset of symptoms and diagnosis of endometriosis. The aim of this study was to evaluate the combined performance of six potential plasma biomarkers in the diagnosis of endometriosis.

METHODS

This case-control study was conducted in 294 infertile women, consisting of 93 women with a normal pelvis and 201 women with endometriosis. We measured plasma concentrations of interleukin (IL)-6, IL-8, tumour necrosis factor-alpha, high-sensitivity C-reactive protein (hsCRP), and cancer antigens CA-125 and CA-19-9. Analyses were done using the Kruskal-Wallis test, Mann-Whitney test, receiver operator characteristic, stepwise logistic regression and least squares support vector machines (LSSVM).

RESULTS

Plasma levels of IL-6, IL-8 and CA-125 were increased in all women with endometriosis and in those with minimal-mild endometriosis, compared with controls. In women with moderate-severe endometriosis, plasma levels of IL-6, IL-8 and CA-125, but also of hsCRP, were significantly higher than in controls. Using stepwise logistic regression, moderate-severe endometriosis was diagnosed with a sensitivity of 100% (specificity 84%) and minimal-mild endometriosis was detected with a sensitivity of 87% (specificity 71%) during the secretory phase. Using LSSVM analysis, minimal-mild endometriosis was diagnosed with a sensitivity of 94% (specificity 61%) during the secretory phase and with a sensitivity of 92% (specificity 63%) during the menstrual phase.

CONCLUSIONS

Advanced statistical analysis of a panel of six selected plasma biomarkers on samples obtained during the secretory phase or during menstruation allows the diagnosis of both minimal-mild and moderate-severe endometriosis with high sensitivity and clinically acceptable specificity.

Authors+Show Affiliations

Department of Obstetrics and Gynaecology, University Hospital Gasthuisberg, Leuven University Fertility Centre, Herestraat 49, B-3000 Leuven, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20007161

Citation

Mihalyi, A, et al. "Non-invasive Diagnosis of Endometriosis Based On a Combined Analysis of Six Plasma Biomarkers." Human Reproduction (Oxford, England), vol. 25, no. 3, 2010, pp. 654-64.
Mihalyi A, Gevaert O, Kyama CM, et al. Non-invasive diagnosis of endometriosis based on a combined analysis of six plasma biomarkers. Hum Reprod. 2010;25(3):654-64.
Mihalyi, A., Gevaert, O., Kyama, C. M., Simsa, P., Pochet, N., De Smet, F., De Moor, B., Meuleman, C., Billen, J., Blanckaert, N., Vodolazkaia, A., Fulop, V., & D'Hooghe, T. M. (2010). Non-invasive diagnosis of endometriosis based on a combined analysis of six plasma biomarkers. Human Reproduction (Oxford, England), 25(3), 654-64. https://doi.org/10.1093/humrep/dep425
Mihalyi A, et al. Non-invasive Diagnosis of Endometriosis Based On a Combined Analysis of Six Plasma Biomarkers. Hum Reprod. 2010;25(3):654-64. PubMed PMID: 20007161.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Non-invasive diagnosis of endometriosis based on a combined analysis of six plasma biomarkers. AU - Mihalyi,A, AU - Gevaert,O, AU - Kyama,C M, AU - Simsa,P, AU - Pochet,N, AU - De Smet,F, AU - De Moor,B, AU - Meuleman,C, AU - Billen,J, AU - Blanckaert,N, AU - Vodolazkaia,A, AU - Fulop,V, AU - D'Hooghe,T M, Y1 - 2009/12/09/ PY - 2009/12/17/entrez PY - 2009/12/17/pubmed PY - 2010/4/24/medline SP - 654 EP - 64 JF - Human reproduction (Oxford, England) JO - Hum Reprod VL - 25 IS - 3 N2 - BACKGROUND: Lack of a non-invasive diagnostic test contributes to the long delay between onset of symptoms and diagnosis of endometriosis. The aim of this study was to evaluate the combined performance of six potential plasma biomarkers in the diagnosis of endometriosis. METHODS: This case-control study was conducted in 294 infertile women, consisting of 93 women with a normal pelvis and 201 women with endometriosis. We measured plasma concentrations of interleukin (IL)-6, IL-8, tumour necrosis factor-alpha, high-sensitivity C-reactive protein (hsCRP), and cancer antigens CA-125 and CA-19-9. Analyses were done using the Kruskal-Wallis test, Mann-Whitney test, receiver operator characteristic, stepwise logistic regression and least squares support vector machines (LSSVM). RESULTS: Plasma levels of IL-6, IL-8 and CA-125 were increased in all women with endometriosis and in those with minimal-mild endometriosis, compared with controls. In women with moderate-severe endometriosis, plasma levels of IL-6, IL-8 and CA-125, but also of hsCRP, were significantly higher than in controls. Using stepwise logistic regression, moderate-severe endometriosis was diagnosed with a sensitivity of 100% (specificity 84%) and minimal-mild endometriosis was detected with a sensitivity of 87% (specificity 71%) during the secretory phase. Using LSSVM analysis, minimal-mild endometriosis was diagnosed with a sensitivity of 94% (specificity 61%) during the secretory phase and with a sensitivity of 92% (specificity 63%) during the menstrual phase. CONCLUSIONS: Advanced statistical analysis of a panel of six selected plasma biomarkers on samples obtained during the secretory phase or during menstruation allows the diagnosis of both minimal-mild and moderate-severe endometriosis with high sensitivity and clinically acceptable specificity. SN - 1460-2350 UR - https://www.unboundmedicine.com/medline/citation/20007161/Non_invasive_diagnosis_of_endometriosis_based_on_a_combined_analysis_of_six_plasma_biomarkers_ L2 - https://academic.oup.com/humrep/article-lookup/doi/10.1093/humrep/dep425 DB - PRIME DP - Unbound Medicine ER -