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Giant Marseillevirus highlights the role of amoebae as a melting pot in emergence of chimeric microorganisms.
Proc Natl Acad Sci U S A. 2009 Dec 22; 106(51):21848-53.PN

Abstract

Giant viruses such as Mimivirus isolated from amoeba found in aquatic habitats show biological sophistication comparable to that of simple cellular life forms and seem to evolve by similar mechanisms, including extensive gene duplication and horizontal gene transfer (HGT), possibly in part through a viral parasite, the virophage. We report here the isolation of "Marseille" virus, a previously uncharacterized giant virus of amoeba. The virions of Marseillevirus encompass a 368-kb genome, a minimum of 49 proteins, and some messenger RNAs. Phylogenetic analysis of core genes indicates that Marseillevirus is the prototype of a family of nucleocytoplasmic large DNA viruses (NCLDV) of eukaryotes. The genome repertoire of the virus is composed of typical NCLDV core genes and genes apparently obtained from eukaryotic hosts and their parasites or symbionts, both bacterial and viral. We propose that amoebae are "melting pots" of microbial evolution where diverse forms emerge, including giant viruses with complex gene repertoires of various origins.

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Authors+Show Affiliations

Boyer M
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Centre National de la Recherche Scientifique, Unité Mixte de Recherche, Institut de Recherche pour le Développement 6236, Faculté de Médecine, Université de la Méditerranée, 13385 Marseille Cedex 5, France.
Yutin N
No affiliation info available
Pagnier I
No affiliation info available
Barrassi L
No affiliation info available
Fournous G
No affiliation info available
Espinosa L
No affiliation info available
Robert C
No affiliation info available
Azza S
No affiliation info available
Sun S
No affiliation info available
Rossmann MG
No affiliation info available
Suzan-Monti M
No affiliation info available
La Scola B
No affiliation info available
Koonin EV
No affiliation info available
Raoult D
No affiliation info available

MeSH

AmoebaChimeraGenome, ViralMicroscopy, ElectronMimiviridaeMolecular Sequence DataPhylogeny

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20007369

Citation

Boyer, Mickaël, et al. "Giant Marseillevirus Highlights the Role of Amoebae as a Melting Pot in Emergence of Chimeric Microorganisms." Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 51, 2009, pp. 21848-53.
Boyer M, Yutin N, Pagnier I, et al. Giant Marseillevirus highlights the role of amoebae as a melting pot in emergence of chimeric microorganisms. Proc Natl Acad Sci U S A. 2009;106(51):21848-53.
Boyer, M., Yutin, N., Pagnier, I., Barrassi, L., Fournous, G., Espinosa, L., Robert, C., Azza, S., Sun, S., Rossmann, M. G., Suzan-Monti, M., La Scola, B., Koonin, E. V., & Raoult, D. (2009). Giant Marseillevirus highlights the role of amoebae as a melting pot in emergence of chimeric microorganisms. Proceedings of the National Academy of Sciences of the United States of America, 106(51), 21848-53. https://doi.org/10.1073/pnas.0911354106
Boyer M, et al. Giant Marseillevirus Highlights the Role of Amoebae as a Melting Pot in Emergence of Chimeric Microorganisms. Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21848-53. PubMed PMID: 20007369.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Giant Marseillevirus highlights the role of amoebae as a melting pot in emergence of chimeric microorganisms. AU - Boyer,Mickaël, AU - Yutin,Natalya, AU - Pagnier,Isabelle, AU - Barrassi,Lina, AU - Fournous,Ghislain, AU - Espinosa,Leon, AU - Robert,Catherine, AU - Azza,Saïd, AU - Sun,Siyang, AU - Rossmann,Michael G, AU - Suzan-Monti,Marie, AU - La Scola,Bernard, AU - Koonin,Eugene V, AU - Raoult,Didier, Y1 - 2009/12/09/ PY - 2009/12/17/entrez PY - 2009/12/17/pubmed PY - 2010/2/19/medline SP - 21848 EP - 53 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 106 IS - 51 N2 - Giant viruses such as Mimivirus isolated from amoeba found in aquatic habitats show biological sophistication comparable to that of simple cellular life forms and seem to evolve by similar mechanisms, including extensive gene duplication and horizontal gene transfer (HGT), possibly in part through a viral parasite, the virophage. We report here the isolation of "Marseille" virus, a previously uncharacterized giant virus of amoeba. The virions of Marseillevirus encompass a 368-kb genome, a minimum of 49 proteins, and some messenger RNAs. Phylogenetic analysis of core genes indicates that Marseillevirus is the prototype of a family of nucleocytoplasmic large DNA viruses (NCLDV) of eukaryotes. The genome repertoire of the virus is composed of typical NCLDV core genes and genes apparently obtained from eukaryotic hosts and their parasites or symbionts, both bacterial and viral. We propose that amoebae are "melting pots" of microbial evolution where diverse forms emerge, including giant viruses with complex gene repertoires of various origins. SN - 1091-6490 UR - https://www.unboundmedicine.com/medline/citation/20007369/Giant_Marseillevirus_highlights_the_role_of_amoebae_as_a_melting_pot_in_emergence_of_chimeric_microorganisms_ L2 - https://www.pnas.org/doi/10.1073/pnas.0911354106?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -