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Cholecalciferol supplementation alters calcitriol-responsive monocyte proteins and decreases inflammatory cytokines in ESRD.
J Am Soc Nephrol. 2010 Feb; 21(2):353-61.JA

Abstract

In vitro, monocyte 1alpha-hydroxylase converts 25-hydroxyvitamin D [25(OH)D] to 1,25-dihydroxyvitamin D to regulate local innate immune responses, but whether 25(OH)D repletion affects vitamin D-responsive monocyte pathways in vivo is unknown. Here, we identified seven patients who had 25(OH)D insufficiency and were undergoing long-term hemodialysis and assessed changes after cholecalciferol and paricalcitol therapies in both vitamin D-responsive proteins in circulating monocytes and serum levels of inflammatory cytokines. Cholecalciferol therapy increased serum 25(OH)D levels four-fold, monocyte vitamin D receptor expression three-fold, and 24-hydroxylase expression; therapy decreased monocyte 1alpha-hydroxylase levels. The CD16(+) "inflammatory" monocyte subset responded to 25(OH)D repletion the most, demonstrating the greatest increase in vitamin D receptor expression after cholecalciferol. Cholecalciferol therapy reduced circulating levels of inflammatory cytokines, including IL-8, IL-6, and TNF. These data suggest that nutritional vitamin D therapy has a biologic effect on circulating monocytes and associated inflammatory markers in patients with ESRD.

Authors+Show Affiliations

3901 Rainbow Boulevard, MS 3002, Kansas City, KS 66160, USA. jstubbs@kumc.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

20007751

Citation

Stubbs, Jason R., et al. "Cholecalciferol Supplementation Alters Calcitriol-responsive Monocyte Proteins and Decreases Inflammatory Cytokines in ESRD." Journal of the American Society of Nephrology : JASN, vol. 21, no. 2, 2010, pp. 353-61.
Stubbs JR, Idiculla A, Slusser J, et al. Cholecalciferol supplementation alters calcitriol-responsive monocyte proteins and decreases inflammatory cytokines in ESRD. J Am Soc Nephrol. 2010;21(2):353-61.
Stubbs, J. R., Idiculla, A., Slusser, J., Menard, R., & Quarles, L. D. (2010). Cholecalciferol supplementation alters calcitriol-responsive monocyte proteins and decreases inflammatory cytokines in ESRD. Journal of the American Society of Nephrology : JASN, 21(2), 353-61. https://doi.org/10.1681/ASN.2009040451
Stubbs JR, et al. Cholecalciferol Supplementation Alters Calcitriol-responsive Monocyte Proteins and Decreases Inflammatory Cytokines in ESRD. J Am Soc Nephrol. 2010;21(2):353-61. PubMed PMID: 20007751.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cholecalciferol supplementation alters calcitriol-responsive monocyte proteins and decreases inflammatory cytokines in ESRD. AU - Stubbs,Jason R, AU - Idiculla,Arun, AU - Slusser,Joyce, AU - Menard,Rochelle, AU - Quarles,L Darryl, Y1 - 2009/12/10/ PY - 2009/12/17/entrez PY - 2009/12/17/pubmed PY - 2010/3/17/medline SP - 353 EP - 61 JF - Journal of the American Society of Nephrology : JASN JO - J. Am. Soc. Nephrol. VL - 21 IS - 2 N2 - In vitro, monocyte 1alpha-hydroxylase converts 25-hydroxyvitamin D [25(OH)D] to 1,25-dihydroxyvitamin D to regulate local innate immune responses, but whether 25(OH)D repletion affects vitamin D-responsive monocyte pathways in vivo is unknown. Here, we identified seven patients who had 25(OH)D insufficiency and were undergoing long-term hemodialysis and assessed changes after cholecalciferol and paricalcitol therapies in both vitamin D-responsive proteins in circulating monocytes and serum levels of inflammatory cytokines. Cholecalciferol therapy increased serum 25(OH)D levels four-fold, monocyte vitamin D receptor expression three-fold, and 24-hydroxylase expression; therapy decreased monocyte 1alpha-hydroxylase levels. The CD16(+) "inflammatory" monocyte subset responded to 25(OH)D repletion the most, demonstrating the greatest increase in vitamin D receptor expression after cholecalciferol. Cholecalciferol therapy reduced circulating levels of inflammatory cytokines, including IL-8, IL-6, and TNF. These data suggest that nutritional vitamin D therapy has a biologic effect on circulating monocytes and associated inflammatory markers in patients with ESRD. SN - 1533-3450 UR - https://www.unboundmedicine.com/medline/citation/20007751/Cholecalciferol_supplementation_alters_calcitriol_responsive_monocyte_proteins_and_decreases_inflammatory_cytokines_in_ESRD_ L2 - http://jasn.asnjournals.org/cgi/pmidlookup?view=long&pmid=20007751 DB - PRIME DP - Unbound Medicine ER -