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Assay of T- and NK-cell subsets and the expression of NKG2A and NKG2D in patients with new-onset systemic lupus erythematosus.

Abstract

This study aims to explore the percentage of T-cell and NK-cell subsets, the expression of NKG2A and NKG2D on CD3+ T cells and CD3-CD56+ NK cells on the total lymphocytes in new-onset systemic lupus erythematosus (SLE) patients, and explore clinical significance of these cell subsets. Thirty-two SLE patients and 32 normal controls were enrolled. Flow cytometry was used to count T- and NK-cell subsets and to detect the expression of NKG2A and NKG2D on CD3+ T cells and CD3-CD56+ NK cells in patients with new-onset SLE. Results show that CD4+ T (t = 2.04, P < 0.05), CD4+/CD8+ T cell (t = 2.66, P < 0.05), CD4+ CD25+ T (t = 2.48, P < 0.05), CD3+CD56+ natural killer T (NKT) (t = 40.05, P < 0.01), CD3-CD56+CD16+ NK-cell subsets (t = 3.50, P < 0.01) were significantly decreased, CD8+ T-cell subsets was significantly increased in patients with new-onset SLE (t = 3.80, P < 0.01), as compared with healthy controls. CD8+ T-cell subset was significantly increased in patients with vasculitis (t = 2.47, P < 0.05), and CD3-CD56+CD16+ NK was increased in patients with arthritis (t = 3.21, P < 0.01). However, no statistically significant correlation was found among different PBMC subsets and SLEDAI activity scores. Patients with SLE had a lower expression of NKG2A (U = 2.42, P < 0.05) as well as NKG2A/NKG2D ratio (t = 2.61, P < 0.05) and a higher expression of NKG2D (t = 2.21, P < 0.05) on CD3+ T cells, compared with normal controls. However, they had a higher expression of NKG2A (t = 2.59, P < 0.05) as well as NKG2A/NKG2D ratio (t = 49.45, P < 0.01) and a lower expression of NKG2D (t = 3.05, P < 0.01) on CD3-CD56+ NK cells. Taken together, the findings indicate the decreased CD4+ T-cell, CD4+/CD8+ T-cell, CD4+CD25+ T-cell, CD3+CD56+ NKT-, and CD3-CD56+CD16+ NK-cell subsets, increased CD8+ T-cell subsets as well as the abnormal expression of NKG2A and NKG2D on CD3+ T and CD3-CD56 + NK cells may play a role in the etiology of SLE.

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  • Authors+Show Affiliations

    ,

    Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, Anhui, 230032, People's Republic of China.

    , , , , , , ,

    Source

    Clinical rheumatology 29:3 2010 Mar pg 315-23

    MeSH

    Adolescent
    Adult
    Age of Onset
    CD3 Complex
    CD56 Antigen
    Female
    Flow Cytometry
    Humans
    Killer Cells, Natural
    Lupus Erythematosus, Systemic
    Lymphocyte Count
    Male
    NK Cell Lectin-Like Receptor Subfamily C
    NK Cell Lectin-Like Receptor Subfamily K
    T-Lymphocyte Subsets
    Young Adult

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    20012119

    Citation

    Li, Wen-Xian, et al. "Assay of T- and NK-cell Subsets and the Expression of NKG2A and NKG2D in Patients With New-onset Systemic Lupus Erythematosus." Clinical Rheumatology, vol. 29, no. 3, 2010, pp. 315-23.
    Li WX, Pan HF, Hu JL, et al. Assay of T- and NK-cell subsets and the expression of NKG2A and NKG2D in patients with new-onset systemic lupus erythematosus. Clin Rheumatol. 2010;29(3):315-23.
    Li, W. X., Pan, H. F., Hu, J. L., Wang, C. Z., Zhang, N., Li, J., ... Ye, D. Q. (2010). Assay of T- and NK-cell subsets and the expression of NKG2A and NKG2D in patients with new-onset systemic lupus erythematosus. Clinical Rheumatology, 29(3), pp. 315-23. doi:10.1007/s10067-009-1322-9.
    Li WX, et al. Assay of T- and NK-cell Subsets and the Expression of NKG2A and NKG2D in Patients With New-onset Systemic Lupus Erythematosus. Clin Rheumatol. 2010;29(3):315-23. PubMed PMID: 20012119.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Assay of T- and NK-cell subsets and the expression of NKG2A and NKG2D in patients with new-onset systemic lupus erythematosus. AU - Li,Wen-Xian, AU - Pan,Hai-Feng, AU - Hu,Jian-Li, AU - Wang,Chang-Zhong, AU - Zhang,Ning, AU - Li,Jing, AU - Li,Xiang-Pei, AU - Xu,Jian-Hua, AU - Ye,Dong-Qing, Y1 - 2009/12/10/ PY - 2009/04/19/received PY - 2009/11/13/accepted PY - 2009/09/23/revised PY - 2009/12/17/entrez PY - 2009/12/17/pubmed PY - 2010/4/14/medline SP - 315 EP - 23 JF - Clinical rheumatology JO - Clin. Rheumatol. VL - 29 IS - 3 N2 - This study aims to explore the percentage of T-cell and NK-cell subsets, the expression of NKG2A and NKG2D on CD3+ T cells and CD3-CD56+ NK cells on the total lymphocytes in new-onset systemic lupus erythematosus (SLE) patients, and explore clinical significance of these cell subsets. Thirty-two SLE patients and 32 normal controls were enrolled. Flow cytometry was used to count T- and NK-cell subsets and to detect the expression of NKG2A and NKG2D on CD3+ T cells and CD3-CD56+ NK cells in patients with new-onset SLE. Results show that CD4+ T (t = 2.04, P < 0.05), CD4+/CD8+ T cell (t = 2.66, P < 0.05), CD4+ CD25+ T (t = 2.48, P < 0.05), CD3+CD56+ natural killer T (NKT) (t = 40.05, P < 0.01), CD3-CD56+CD16+ NK-cell subsets (t = 3.50, P < 0.01) were significantly decreased, CD8+ T-cell subsets was significantly increased in patients with new-onset SLE (t = 3.80, P < 0.01), as compared with healthy controls. CD8+ T-cell subset was significantly increased in patients with vasculitis (t = 2.47, P < 0.05), and CD3-CD56+CD16+ NK was increased in patients with arthritis (t = 3.21, P < 0.01). However, no statistically significant correlation was found among different PBMC subsets and SLEDAI activity scores. Patients with SLE had a lower expression of NKG2A (U = 2.42, P < 0.05) as well as NKG2A/NKG2D ratio (t = 2.61, P < 0.05) and a higher expression of NKG2D (t = 2.21, P < 0.05) on CD3+ T cells, compared with normal controls. However, they had a higher expression of NKG2A (t = 2.59, P < 0.05) as well as NKG2A/NKG2D ratio (t = 49.45, P < 0.01) and a lower expression of NKG2D (t = 3.05, P < 0.01) on CD3-CD56+ NK cells. Taken together, the findings indicate the decreased CD4+ T-cell, CD4+/CD8+ T-cell, CD4+CD25+ T-cell, CD3+CD56+ NKT-, and CD3-CD56+CD16+ NK-cell subsets, increased CD8+ T-cell subsets as well as the abnormal expression of NKG2A and NKG2D on CD3+ T and CD3-CD56 + NK cells may play a role in the etiology of SLE. SN - 1434-9949 UR - https://www.unboundmedicine.com/medline/citation/20012119/Assay_of_T__and_NK_cell_subsets_and_the_expression_of_NKG2A_and_NKG2D_in_patients_with_new_onset_systemic_lupus_erythematosus_ L2 - https://dx.doi.org/10.1007/s10067-009-1322-9 DB - PRIME DP - Unbound Medicine ER -