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FLO gene-dependent phenotypes in industrial wine yeast strains.
Appl Microbiol Biotechnol. 2010 Apr; 86(3):931-45.AM

Abstract

Most commercial yeast strains are nonflocculent. However, controlled flocculation phenotypes could provide significant benefits to many fermentation-based industries. In nonflocculent laboratory strains, it has been demonstrated that it is possible to adjust flocculation and adhesion phenotypes to desired specifications by altering expression of the otherwise silent but dominant flocculation (FLO) genes. However, FLO genes are characterized by high allele heterogeneity and are subjected to epigenetic regulation. Extrapolation of data obtained in laboratory strains to industrial strains may therefore not always be applicable. Here, we assess the adhesion phenotypes that are associated with the expression of a chromosomal copy of the FLO1, FLO5, or FLO11 open reading frame in two nonflocculent commercial wine yeast strains, BM45 and VIN13. The chromosomal promoters of these genes were replaced with stationary phase-inducible promoters of the HSP30 and ADH2 genes. Under standard laboratory and wine making conditions, the strategy resulted in expected and stable expression patterns of these genes in both strains. However, the specific impact of the expression of individual FLO genes showed significant differences between the two wine strains and with corresponding phenotypes in laboratory strains. The data suggest that optimization of the flocculation pattern of individual commercial strains will have to be based on a strain-by-strain approach.

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Authors+Show Affiliations

Govender P
Department of Biochemistry, University of KwaZulu-Natal, Durban, South Africa.
Bester M
No affiliation info available
Bauer FF
No affiliation info available

MeSH

Cell AdhesionDNA, FungalFermentationIndustrial MicrobiologyLectinsMannose-Binding LectinsMembrane GlycoproteinsMolecular Sequence DataPromoter Regions, GeneticSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Analysis, DNAWine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20013339

Citation

Govender, Patrick, et al. "FLO Gene-dependent Phenotypes in Industrial Wine Yeast Strains." Applied Microbiology and Biotechnology, vol. 86, no. 3, 2010, pp. 931-45.
Govender P, Bester M, Bauer FF. FLO gene-dependent phenotypes in industrial wine yeast strains. Appl Microbiol Biotechnol. 2010;86(3):931-45.
Govender, P., Bester, M., & Bauer, F. F. (2010). FLO gene-dependent phenotypes in industrial wine yeast strains. Applied Microbiology and Biotechnology, 86(3), 931-45. https://doi.org/10.1007/s00253-009-2381-1
Govender P, Bester M, Bauer FF. FLO Gene-dependent Phenotypes in Industrial Wine Yeast Strains. Appl Microbiol Biotechnol. 2010;86(3):931-45. PubMed PMID: 20013339.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - FLO gene-dependent phenotypes in industrial wine yeast strains. AU - Govender,Patrick, AU - Bester,Michael, AU - Bauer,Florian F, Y1 - 2009/12/16/ PY - 2009/10/27/received PY - 2009/11/23/accepted PY - 2009/11/23/revised PY - 2009/12/17/entrez PY - 2009/12/17/pubmed PY - 2010/6/2/medline SP - 931 EP - 45 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 86 IS - 3 N2 - Most commercial yeast strains are nonflocculent. However, controlled flocculation phenotypes could provide significant benefits to many fermentation-based industries. In nonflocculent laboratory strains, it has been demonstrated that it is possible to adjust flocculation and adhesion phenotypes to desired specifications by altering expression of the otherwise silent but dominant flocculation (FLO) genes. However, FLO genes are characterized by high allele heterogeneity and are subjected to epigenetic regulation. Extrapolation of data obtained in laboratory strains to industrial strains may therefore not always be applicable. Here, we assess the adhesion phenotypes that are associated with the expression of a chromosomal copy of the FLO1, FLO5, or FLO11 open reading frame in two nonflocculent commercial wine yeast strains, BM45 and VIN13. The chromosomal promoters of these genes were replaced with stationary phase-inducible promoters of the HSP30 and ADH2 genes. Under standard laboratory and wine making conditions, the strategy resulted in expected and stable expression patterns of these genes in both strains. However, the specific impact of the expression of individual FLO genes showed significant differences between the two wine strains and with corresponding phenotypes in laboratory strains. The data suggest that optimization of the flocculation pattern of individual commercial strains will have to be based on a strain-by-strain approach. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/20013339/FLO_gene_dependent_phenotypes_in_industrial_wine_yeast_strains_ L2 - https://dx.doi.org/10.1007/s00253-009-2381-1 DB - PRIME DP - Unbound Medicine ER -